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MBOAT4 elisa kit :: Human membrane bound O-acyltransferase domain containing 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS929750
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

membrane bound O-acyltransferase domain containing 4 (MBOAT4), ELISA Kit

★Popular Item★
 Also Known As   

Human Ghrelin O-acyltransferase, MBOAT4 ELISA Kit

 Product Synonym Names    Human Ghrelin O-acyltransferase (MBOAT4) ELISA kit; FKSG89; GOAT; OACT4; O-acyltransferase (membrane bound) domain containing 4; ghrelin O-acyltransferase; membrane bound O-acyltransferase domain containing 4
 Product Gene Name   

MBOAT4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS929750 Testing Data    Testing Data PDF
 OMIM    611940
 3D Structure    ModBase 3D Structure for Q96T53
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human MBOAT4. No significant cross-reactivity or interference between human MBOAT4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml -1000 pg/ml.
 Sensitivity    The minimum detectable dose of human MBOAT4 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MBOAT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MBOAT4 purchase    MBS929750 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the membrane bound O-acyltransferase domain containing 4 (MBOAT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/ml in biological research samples containing MBOAT4, with an estimated sensitivity of The minimum detectable dose of human MBOAT4 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS929750 kit is based on MBOAT4 antibody-MBOAT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MBOAT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MBOAT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MBOAT4 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBOAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBOAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBOAT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBOAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of MBOAT4 elisa kit    MBOAT4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS929750. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MBOAT4. It may not necessarily be applicable to this product.
 NCBI GI #    189571629
 NCBI GeneID    619373
 NCBI Accession #    NP_001094386.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001100916.1 [Other Products]
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 UniProt Primary Accession #    Q96T53 [Other Products]
 UniProt Secondary Accession #    B1Q003 [Other Products]
 UniProt Related Accession #    Q96T53 [Other Products]
 Molecular Weight    49,716 Da
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 NCBI Official Full Name    ghrelin O-acyltransferase
 NCBI Official Synonym Full Names    membrane bound O-acyltransferase domain containing 4
 NCBI Official Symbol    MBOAT4 [Similar Products]
 NCBI Official Synonym Symbols   
GOAT; OACT4; FKSG89
[Similar Products]
 NCBI Protein Information    ghrelin O-acyltransferase; O-acyltransferase domain-containing protein 4; O-acyltransferase (membrane bound) domain containing 4; membrane-bound O-acyltransferase domain-containing protein 4
 UniProt Protein Name    Ghrelin O-acyltransferase
 UniProt Synonym Protein Names   
Membrane-bound O-acyltransferase domain-containing protein 4; O-acyltransferase domain-containing protein 4
 Protein Family    Ghrelin O-acyltransferase
 UniProt Gene Name    MBOAT4 [Similar Products]
 UniProt Synonym Gene Names    GOAT; OACT4; FKSG89; O-acyltransferase domain-containing protein 4 [Similar Products]
 UniProt Entry Name    MBOA4_HUMAN
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 UniProt Comments for MBOAT4    MBOAT4: Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Belongs to the membrane-bound acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; EC 2.3.1.-

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Molecular Function: serine O-acyltransferase activity

Biological Process: cellular protein metabolic process; peptidyl-serine octanoylation
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 Research Articles on MBOAT4    1. GOAT is also present in human plasma and GOAT protein levels depend on the metabolic environment with decreased levels in anorexic and increased levels in morbidly obese patients
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MBOAT4 elisa kitDiseases associated with MBOAT4 elisa kit
 Products by Pathway  Pathway Diagram
 Diabetes Pathways antibodies  Diabetes Pathways Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Synthesis, Secretion, And Deacylation Of Ghrelin Pathway antibodies  Synthesis, Secretion, And Deacylation Of Ghrelin Pathway Diagram
 Disease Name  Pubmed Publications
 Eating Disorders Antibodies  >1 publications with MBOAT4 and Eating Disorders
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