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MBP elisa kit :: Human myelin basic protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700083
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

myelin basic protein (MBP), ELISA Kit

 Also Known As   

Human myelin basic protein, MBP ELISA Kit

 Product Synonym Names    Human myelin basic protein; MBP ELISA Kit; MGC99675; Golli-mbp; OTTHUMP00000174383; myelin basic protein
 Product Gene Name   

MBP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS700083 Testing Data    Testing Data PDF
 OMIM    159430
 3D Structure    ModBase 3D Structure for P02686
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human MBP. No significant cross-reactivity or interference between human MBP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates
 Assay Type    Quantitative Sandwich
 Detection Range    0.156 ng/ml-10 ng/ml
 Sensitivity    <0.039 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of MBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MBP purchase    MBS700083 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the myelin basic protein (MBP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing MBP, with an estimated sensitivity of <0.039 ng/ml. The ELISA analytical biochemical technique of the MBS700083 kit is based on MBP antibody-MBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). A key protein of the myelin sheath surround nerve bundles, human myelin basic protein MBP expression is implicated in multiple neuronal disorders. Reduced levels are a hallmark of MS but therapeutic injections reduce infectivity of rabies. Thus, there is considerable interest in basic biology in mammalian species (pig, sheep, mouse, rat, rabbit, guinea pig) and even avian (chicken) proteins. To determine expression in human samples, an MBP ELISA kit, utilizing a quantitative sandwich format and a specific anti human MBP antibody, can accurately assay cell lysates or sera. These ELISA kits display similar results to IHC experiments and are an excellent value with all reagents included. Products packaged with a free datasheet include protein antibody bound to 1 plate hu 96 tests and secondary antibodies - rabbit IgG Alexa fluorâ® or MBS avidin, for detection at ng ml ranges. Order this product and other MBP ELISA kits from MyBiosource.
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Product Description specifically for MBP elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of MBP elisa kit    MBP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700083
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NCBI/Uniprot data below describe general gene information for MBP. It may not necessarily be applicable to this product.
 NCBI GI #    68509930
 NCBI GeneID    4155
 NCBI Accession #    NP_001020252.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001025081.1 [Other Products]
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 UniProt Primary Accession #    P02686 [Other Products]
 UniProt Secondary Accession #    Q15337; Q15338; Q15339; Q15340; A4FU54; A6NI84; A8MY86; A8MYL4; B3KY66; B7ZKS2; B7ZKS4 [Other Products]
 UniProt Related Accession #    P02686 [Other Products]
 Molecular Weight    33,117 Da
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 NCBI Official Full Name    myelin basic protein isoform 1
 NCBI Official Synonym Full Names    myelin basic protein
 NCBI Official Symbol    MBP [Similar Products]
 NCBI Protein Information    Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein
 UniProt Protein Name    Myelin basic protein
 UniProt Synonym Protein Names   
Myelin A1 protein; Myelin membrane encephalitogenic protein
 Protein Family    Myelin basic protein
 UniProt Gene Name    MBP [Similar Products]
 UniProt Synonym Gene Names    MBP [Similar Products]
 UniProt Entry Name    MBP_HUMAN
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 NCBI Summary for MBP    The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MBP    Function: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.1 PublicationManual assertion based on experiment in:Ref.7
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 Research Articles on MBP    1. In autistic children with allergic manifestations there were increased serum levels of MBP (and MAG) compared to those without allergies.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Human myelin basic protein, MBP ELISA Kit
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 MBS053080  Duck Anti-Myelin Basic Protein Antibody ELISA Kit  MBS105615  Sheep Anti-Myelin Basic Protein Antibody ELISA Kit
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Products associated with MBP elisa kitPathways associated with MBP elisa kit
 Reference Product  PubMed Publications
 MAG elisa kit  >170 publications with MBP and MAG
 MAPK1 elisa kit  >51 publications with MBP and MAPK1
 PLP1 elisa kit  >41 publications with MBP and PLP1
 MAPK3 elisa kit  >39 publications with MBP and MAPK3
 HLA-DRA elisa kit  >14 publications with MBP and HLA-DRA
 STK4 elisa kit  >4 publications with MBP and STK4
 RAN elisa kit  >4 publications with MBP and RAN
 PRKCD elisa kit  >2 publications with MBP and PRKCD
 TRAF6 elisa kit  >2 publications with MBP and TRAF6
 PRKCA elisa kit  >2 publications with MBP and PRKCA
 Products by Pathway  Pathway Diagram
 Glial Cell Differentiation Pathway antibodies  Glial Cell Differentiation Pathway Diagram
 MAPK Cascade Pathway antibodies  MAPK Cascade Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
Diseases associated with MBP elisa kitOrgans/Tissues associated with MBP elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2639 publications with MBP and Nervous System Diseases
 Demyelinating Diseases Antibodies  >1950 publications with MBP and Demyelinating Diseases
 Brain Diseases Antibodies  >1699 publications with MBP and Brain Diseases
 Disease Models, Animal Antibodies  >1364 publications with MBP and Disease Models, Animal
 Encephalomyelitis, Autoimmune, Experimental Antibodies  >1180 publications with MBP and Encephalomyelitis, Autoimmune, Experimental
 Cardiovascular Diseases Antibodies  >675 publications with MBP and Cardiovascular Diseases
 Inflammation Antibodies  >477 publications with MBP and Inflammation
 Hypertension Antibodies  >407 publications with MBP and Hypertension
 Necrosis Antibodies  >256 publications with MBP and Necrosis
 Heart Diseases Antibodies  >185 publications with MBP and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3345 publications with MBP and Blood
 Brain Antibodies  >1612 publications with MBP and Brain
 Nerve Antibodies  >836 publications with MBP and Nerve
 Spinal Cord Antibodies  >613 publications with MBP and Spinal Cord
 Heart Antibodies  >599 publications with MBP and Heart
 Kidney Antibodies  >239 publications with MBP and Kidney
 Lymph Node Antibodies  >233 publications with MBP and Lymph Node
 Lymph Antibodies  >233 publications with MBP and Lymph
 Spleen Antibodies  >224 publications with MBP and Spleen
 Muscle Antibodies  >219 publications with MBP and Muscle
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