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MPL elisa kit :: Human myeloproliferative leukemia virus oncogene ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2602843
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

myeloproliferative leukemia virus oncogene (MPL), ELISA Kit

 Also Known As   

Human Thrombopoietin receptor, MPL ELISA Kit

 Product Gene Name   

MPL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604498
 3D Structure    ModBase 3D Structure for P40238
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    human serum, plasma or cell culture supernatant
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/mL
 Intra-assay Precision    ? 8%
 Inter-assay Precision    ? 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MPL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MPL purchase    MBS2602843 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the myeloproliferative leukemia virus oncogene (MPL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing MPL, with an estimated sensitivity of 0.06 ng/mL. The ELISA analytical biochemical technique of the MBS2602843 kit is based on MPL antibody-MPL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as human serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MPL elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MPL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of MPL elisa kit    MPL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2602843. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MPL. It may not necessarily be applicable to this product.
 NCBI GI #    4885491
 NCBI GeneID    4352
 NCBI Accession #    NP_005364.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005373.2 [Other Products]
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 UniProt Primary Accession #    P40238 [Other Products]
 UniProt Secondary Accession #    Q5JUZ0 [Other Products]
 UniProt Related Accession #    P40238 [Other Products]
 Molecular Weight    71,245 Da [Similar Products]
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 NCBI Official Full Name    thrombopoietin receptor
 NCBI Official Synonym Full Names    MPL proto-oncogene, thrombopoietin receptor
 NCBI Official Symbol    MPL [Similar Products]
 NCBI Official Synonym Symbols   
MPLV; TPOR; C-MPL; CD110; THCYT2
[Similar Products]
 NCBI Protein Information    thrombopoietin receptor; TPO-R; proto-oncogene c-Mpl; myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene
 UniProt Protein Name    Thrombopoietin receptor
 UniProt Synonym Protein Names   
Myeloproliferative leukemia protein; Proto-oncogene c-Mpl; CD_antigen: CD110
 Protein Family    Zinc metalloproteinase
 UniProt Gene Name    MPL [Similar Products]
 UniProt Synonym Gene Names    TPOR; TPO-R [Similar Products]
 UniProt Entry Name    TPOR_HUMAN
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 NCBI Summary for MPL    In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MPL    MPL: a hematopoietic receptor for thrombopoietin. Thrombopoietin is the major regulator of megakaryocytopoiesis and platelet formation. Dimerizes upon binding of thrombopoietin, leading to its phosphorylation and activation of JAKs and STATs. May represent a regulatory molecule specific for TPO-R-dependent immune responses. Two splice-variant isoforms have been described.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; transmembrane receptor activity

Biological Process: cell proliferation; platelet activation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; homeostasis of number of cells; blood coagulation; regulation of chemokine production

Disease: Myelofibrosis; Amegakaryocytic Thrombocytopenia, Congenital; Thrombocythemia 2
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 Research Articles on MPL    1. Studies demonstrate that progression to AML is part of the natural history of MPL W515L-associated disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MPL elisa kitPathways associated with MPL elisa kit
 Reference Product  PubMed Publications
 JAK2 elisa kit  >232 publications with MPL and JAK2
 THPO elisa kit  >18 publications with MPL and THPO
 SHC1 elisa kit  >5 publications with MPL and SHC1
 PTPN11 elisa kit  >4 publications with MPL and PTPN11
 IRS2 elisa kit  >2 publications with MPL and IRS2
 SOCS1 elisa kit  >1 publications with MPL and SOCS1
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Platelet Aggregation (Plug Formation) Pathway antibodies  Platelet Aggregation (Plug Formation) Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
Diseases associated with MPL elisa kitOrgans/Tissues associated with MPL elisa kit
 Disease Name  Pubmed Publications
 Hematologic Diseases Antibodies  >516 publications with MPL and Hematologic Diseases
 Bone Marrow Diseases Antibodies  >342 publications with MPL and Bone Marrow Diseases
 Leukemia Antibodies  >250 publications with MPL and Leukemia
 Thrombocytosis Antibodies  >162 publications with MPL and Thrombocytosis
 Thrombocythemia, Essential Antibodies  >142 publications with MPL and Thrombocythemia, Essential
 Leukemia, Myeloid Antibodies  >136 publications with MPL and Leukemia, Myeloid
 Polycythemia Vera Antibodies  >130 publications with MPL and Polycythemia Vera
 Blood Coagulation Disorders Antibodies  >122 publications with MPL and Blood Coagulation Disorders
 Leukemia, Myeloid, Acute Antibodies  >97 publications with MPL and Leukemia, Myeloid, Acute
 Drug Toxicity Antibodies  >64 publications with MPL and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >43 publications with MPL and Lung
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