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MYLK elisa kit :: Human myosin light chain kinase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704311
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

myosin light chain kinase (MYLK), ELISA Kit

★Popular Item★
 Also Known As   

Human myosin light chain kinase, MLCK ELISA Kit

 Product Synonym Names    Human myosin light chain kinase; MLCK ELISA Kit; DKFZp686I10125; FLJ12216; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK; OTTHUMP00000180642; myosin; light polypeptide kinase; smooth muscle myosin light chain kinase; myosin light chain kinase
 Product Gene Name   

MYLK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704311 Testing Data    Testing Data PDF
 OMIM    gene 613780
 3D Structure    ModBase 3D Structure for Q15746
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human MYLK. No significant cross-reactivity or interference between Human MYLK and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Detection Range    0.78 ng/ml-50 ng/ml
 Sensitivity    0.195 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MYLK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MYLK purchase    MBS704311 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the myosin light chain kinase (MYLK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78 ng/ml-50 ng/ml in biological research samples containing MYLK, with an estimated sensitivity of 0.195 ng/ml. The ELISA analytical biochemical technique of the MBS704311 kit is based on MYLK antibody-MYLK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYLK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYLK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MYLK elisa kit

   Introduction: Myosin-light-chain kinase also known as MYLK or MLCK is a serine/threonine-specific protein kinase that phosphorylates the regulatory light chain of myosin II. These enzymes are important in the mechanism of contraction in muscle. Once there is an influx of calcium cations (Ca++) into the muscle, either from the sarcoplasmic reticulum or, more importantly, from the extracellular space, contraction of smooth muscle fibers may begin. First, the calcium will bind to calmodulin. This binding will activate MLCK, which will go on to phosphorylate the myosin light chain at serine residue 19. This will enable the myosin crossbridge to bind to the actin filament and allow contraction to begin (through the crossbridge cycle). Since smooth muscle does not contain a troponin complex like striated muscle does, this mechanism is the main pathway for regulating smooth muscle contraction.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MLCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MLCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MLCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MLCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MYLK elisa kit    MYLK elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704311. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MYLK. It may not necessarily be applicable to this product.
 NCBI GI #    116008192
 NCBI GeneID    4638
 NCBI Accession #    NP_444253.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_053025.3 [Other Products]
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 UniProt Primary Accession #    Q15746 [Other Products]
 UniProt Secondary Accession #    O95796; O95797; O95798; O95799; Q14844; Q16794; Q17S15; Q3ZCP9; Q5MY99; B4DUE3; D3DN97 [Other Products]
 UniProt Related Accession #    Q15746 [Other Products]
 Molecular Weight    210,715 Da
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 NCBI Official Full Name    myosin light chain kinase, smooth muscle isoform 1
 NCBI Official Synonym Full Names    myosin light chain kinase
 NCBI Official Symbol    MYLK [Similar Products]
 NCBI Official Synonym Symbols   
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
[Similar Products]
 NCBI Protein Information    myosin light chain kinase, smooth muscle; telokin; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
 UniProt Protein Name    Myosin light chain kinase, smooth muscle
 UniProt Synonym Protein Names   
Kinase-related protein; KRP; TelokinCleaved into the following chain:Myosin light chain kinase, smooth muscle, deglutamylated form
 Protein Family    Myosin light chain kinase
 UniProt Gene Name    MYLK [Similar Products]
 UniProt Synonym Gene Names    MLCK; MLCK1; MYLK1; MLCK; smMLCK; KRP [Similar Products]
 UniProt Entry Name    MYLK_HUMAN
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 NCBI Summary for MYLK    This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MYLK    Function: Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca2+ entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis.13 PublicationsManual assertion based on experiment in:Ref.4

Catalytic activity: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Cofactor: Magnesium.

Enzyme regulation: Isoform 1 is activated by phosphorylation on Tyr-464 and Tyr-471. Isoforms which lack these tyrosine residues are not regulated in this way. All catalytically active isoforms require binding to calcium and calmodulin for activation. Repressed by organometallic pyridylnaphthalimide complexes, wortmannin, ML-7 (a synthetic naphthalenesulphonyl derivative that inhibits the binding of ATP to MLCK) and ML-9.6 PublicationsManual assertion based on experiment in:Ref.4
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 Research Articles on MYLK    1. Results suggest that Aurora B, but not Rho/MLCK (myosin-light-chain kinase) signaling, is essential for the localization of 2P-MRLC (myosin regulatory light chains) to the midzone in dividing HeLa cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MYLK elisa kitDiseases associated with MYLK elisa kit
 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Endothelin Pathway antibodies  Endothelin Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 Disease Name  Pubmed Publications
 Vascular Diseases Antibodies  >9 publications with MYLK and Vascular Diseases
 Acute Lung Injury Antibodies  >9 publications with MYLK and Acute Lung Injury
 Inflammation Antibodies  >8 publications with MYLK and Inflammation
 Hypersensitivity Antibodies  >7 publications with MYLK and Hypersensitivity
 Pneumonia Antibodies  >4 publications with MYLK and Pneumonia
 Edema Antibodies  >3 publications with MYLK and Edema
 Hypertension Antibodies  >3 publications with MYLK and Hypertension
 Atherosclerosis Antibodies  >3 publications with MYLK and Atherosclerosis
 Disease Models, Animal Antibodies  >3 publications with MYLK and Disease Models, Animal
 Breast Neoplasms Antibodies  >3 publications with MYLK and Breast Neoplasms
Organs/Tissues associated with MYLK elisa kit
 Organ/Tissue Name  Pubmed Publications
 Head Antibodies  >2 publications with MYLK and Head
 Brain Antibodies  >2 publications with MYLK and Brain
 Liver Antibodies  >1 publications with MYLK and Liver
 Spleen Antibodies  >1 publications with MYLK and Spleen
 Heart Antibodies  >1 publications with MYLK and Heart
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