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NLK elisa kit :: Human nemo-like kinase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9336671 NLK elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

nemo-like kinase (NLK), ELISA Kit

 Also Known As   

Human Serine/threonine-protein kinase NLK, NLK ELISA Kit

 Product Synonym Names    Human Serine/threonine-protein kinase NLK (NLK) ELISA kit; DKFZp761G1211; FLJ21033; nemo like kinase; nemo-like kinase
 Product Gene Name   

NLK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609476
 3D Structure    ModBase 3D Structure for Q9UBE8
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between Human NLK and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    0.25ng/ml-8ng/ml.
 Sensitivity    0.1ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NLK concentrations in Human serum, plasma and other body fluids. Using Purified Human NLK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NLK and NLK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NLK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NLK purchase    MBS9336671 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nemo-like kinase (NLK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25ng/ml-8ng/ml in biological research samples containing NLK, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS9336671 kit is based on NLK antibody-NLK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NLK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NLK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NLK elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NLK concentrations in Human serum, plasma and other body fluids. Using Purified Human NLK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NLK and NLK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS9336671. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NLK. It may not necessarily be applicable to this product.
 NCBI GI #    149408126
 NCBI GeneID    51701
 NCBI Accession #    NP_057315.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_016231.4 [Other Products]
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 UniProt Primary Accession #    Q9UBE8 [Other Products]
 UniProt Secondary Accession #    Q2PNI9; Q6P2A3; B2RCX1 [Other Products]
 UniProt Related Accession #    Q9UBE8 [Other Products]
 Molecular Weight    58,283 Da
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 NCBI Official Full Name    serine/threonine-protein kinase NLK
 NCBI Official Synonym Full Names    nemo-like kinase
 NCBI Official Symbol    NLK [Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase NLK
 UniProt Protein Name    Serine/threonine-protein kinase NLK
 UniProt Synonym Protein Names   
Nemo-like kinase; Protein LAK1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    NLK [Similar Products]
 UniProt Synonym Gene Names    LAK1 [Similar Products]
 UniProt Entry Name    NLK_HUMAN
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 UniProt Comments for NLK    NLK: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; CMGC group; MAPK family; nmo subfamily

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; magnesium ion binding; SH2 domain binding; transcription factor binding; ATP binding; protein kinase activity

Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; MAPKKK cascade; protein amino acid phosphorylation; serine phosphorylation of STAT3 protein
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 Research Articles on NLK    1. NLK functions as a pivotal negative regulator of NF-kappaB via disrupting the interaction of TAK1 with IKKbeta.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NLK elisa kitPathways associated with NLK elisa kit
 Reference Product  PubMed Publications
 STAT3 elisa kit  >4 publications with NLK and STAT3
 MYB elisa kit  >4 publications with NLK and MYB
 LEF1 elisa kit  >4 publications with NLK and LEF1
 HIPK2 elisa kit  >4 publications with NLK and HIPK2
 CREBBP elisa kit  >1 publications with NLK and CREBBP
 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Noncanonical Wnt Signaling Pathway antibodies  Noncanonical Wnt Signaling Pathway Diagram
 Presenilin Action In Notch And Wnt Signaling Pathway antibodies  Presenilin Action In Notch And Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway NetPath antibodies  Wnt Signaling Pathway NetPath Diagram
Diseases associated with NLK elisa kitOrgans/Tissues associated with NLK elisa kit
 Disease Name  Pubmed Publications
 Liver Neoplasms Antibodies  >5 publications with NLK and Liver Neoplasms
 Liver Diseases Antibodies  >4 publications with NLK and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with NLK and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >3 publications with NLK and Nervous System Diseases
 Intestinal Neoplasms Antibodies  >3 publications with NLK and Intestinal Neoplasms
 Anemia Antibodies  >2 publications with NLK and Anemia
 Immune System Diseases Antibodies  >2 publications with NLK and Immune System Diseases
 Lung Neoplasms Antibodies  >2 publications with NLK and Lung Neoplasms
 Inflammation Antibodies  >2 publications with NLK and Inflammation
 Brain Diseases Antibodies  >1 publications with NLK and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with NLK and Bone
 Brain Antibodies  >12 publications with NLK and Brain
 Embryonic Tissue Antibodies  >9 publications with NLK and Embryonic Tissue
 Liver Antibodies  >8 publications with NLK and Liver
 Bone Marrow Antibodies  >6 publications with NLK and Bone Marrow
 Blood Antibodies  >5 publications with NLK and Blood
 Kidney Antibodies  >4 publications with NLK and Kidney
 Heart Antibodies  >4 publications with NLK and Heart
 Muscle Antibodies  >4 publications with NLK and Muscle
 Eye Antibodies  >4 publications with NLK and Eye
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