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NGF elisa kit :: Human nerve growth factor (beta polypeptide) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS914982
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

nerve growth factor (beta polypeptide) (NGF), ELISA Kit

★Popular Item★
 Also Known As   

Human Nerve growth factor, NGF ELISA Kit

 Product Synonym Names    Human Nerve growth factor; NGF ELISA kit; Beta-NGF; HSAN5; MGC161426; MGC161428; NGFB; beta-nerve growth factor; nerve growth factor; beta polypeptide; nerve growth factor; beta subunit; nerve growth factor (beta polypeptide)
 Product Gene Name   

NGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS914982 Testing Data    Testing Data PDF
 OMIM    gene 608654
 3D Structure    ModBase 3D Structure for P01138
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NGF. No significant cross-reactivity or interference between human NGF and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates, cell lysates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    78 pg/ml-5000 pg/ml.
 Sensitivity    19.5 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NGF purchase    MBS914982 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nerve growth factor (beta polypeptide) (NGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78 pg/ml-5000 pg/ml in biological research samples containing NGF, with an estimated sensitivity of 19.5 pg/ml. The ELISA analytical biochemical technique of the MBS914982 kit is based on NGF antibody-NGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NGF elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NGF elisa kit    NGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS914982. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NGF. It may not necessarily be applicable to this product.
 NCBI GI #    70995319
 NCBI GeneID    4803
 NCBI Accession #    NP_002497.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002506.2 [Other Products]
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 UniProt Primary Accession #    P01138 [Other Products]
 UniProt Secondary Accession #    Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5 [Other Products]
 UniProt Related Accession #    P01138 [Other Products]
 Molecular Weight    26,959 Da
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 NCBI Official Full Name    beta-nerve growth factor
 NCBI Official Synonym Full Names    nerve growth factor (beta polypeptide)
 NCBI Official Symbol    NGF [Similar Products]
 NCBI Official Synonym Symbols   
NGFB; HSAN5; Beta-NGF
[Similar Products]
 NCBI Protein Information    beta-nerve growth factor; nerve growth factor, beta subunit
 UniProt Protein Name    Beta-nerve growth factor
 Protein Family    Nerve growth factor
 UniProt Gene Name    NGF [Similar Products]
 UniProt Synonym Gene Names    NGFB; Beta-NGF [Similar Products]
 UniProt Entry Name    NGF_HUMAN
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 NCBI Summary for NGF    This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NGF    NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity

Biological Process: circadian rhythm; response to nicotine; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of apoptosis; adult locomotory behavior; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; sensory perception of pain; regulation of axonogenesis; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to electrical stimulus; inflammatory response; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; memory; regulation of release of sequestered calcium ion into cytosol; positive regulation of nerve growth factor receptor signaling pathway; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; response to mechanical stimulus; phospholipase C activation; response to ozone; Ras protein signal transduction; positive regulation of transcription factor activity; positive regulation of axonogenesis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V
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 Research Articles on NGF    1. Silencing beta-NGF gene expression by siRNA suppresses the growth of endometriotic implants.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NGF elisa kitPathways associated with NGF elisa kit
 Reference Product  PubMed Publications
 NTRK1 elisa kit  >1822 publications with NGF and NTRK1
 NGFR elisa kit  >220 publications with NGF and NGFR
 PLG elisa kit  >7 publications with NGF and PLG
 MMP7 elisa kit  >1 publications with NGF and MMP7
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of TRKA Receptors Pathway antibodies  Activation Of TRKA Receptors Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Axonal Growth Stimulation Pathway antibodies  Axonal Growth Stimulation Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 Ceramide Signalling Pathway antibodies  Ceramide Signalling Pathway Diagram
 Frs2-mediated Activation Pathway antibodies  Frs2-mediated Activation Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
Diseases associated with NGF elisa kitOrgans/Tissues associated with NGF elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1909 publications with NGF and Nervous System Diseases
 Brain Diseases Antibodies  >1014 publications with NGF and Brain Diseases
 Disease Models, Animal Antibodies  >664 publications with NGF and Disease Models, Animal
 Pain Antibodies  >569 publications with NGF and Pain
 Inflammation Antibodies  >568 publications with NGF and Inflammation
 Nerve Degeneration Antibodies  >550 publications with NGF and Nerve Degeneration
 Peripheral Nervous System Diseases Antibodies  >410 publications with NGF and Peripheral Nervous System Diseases
 Drug Toxicity Antibodies  >400 publications with NGF and Drug Toxicity
 Necrosis Antibodies  >396 publications with NGF and Necrosis
 Cardiovascular Diseases Antibodies  >296 publications with NGF and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >577 publications with NGF and Embryonic Tissue
 Muscle Antibodies  >564 publications with NGF and Muscle
 Skin Antibodies  >476 publications with NGF and Skin
 Eye Antibodies  >392 publications with NGF and Eye
 Lung Antibodies  >139 publications with NGF and Lung
 Intestine Antibodies  >105 publications with NGF and Intestine
 Spleen Antibodies  >88 publications with NGF and Spleen
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