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NBL1 elisa kit :: Human neuroblastoma, suppression of tumorigenicity 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS927613
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

neuroblastoma, suppression of tumorigenicity 1 (NBL1), ELISA Kit

★Popular Item★
 Also Known As   

Human Neuroblastoma suppressor of tumorigenicity 1, NBL1 ELISA Kit

 Product Synonym Names    Human Neuroblastoma suppressor of tumorigenicity 1 (NBL1) ELISA kit; RP5-1056L3.4; D1S1733E; DAN; DAND1; MGC8972; NB; NO3; differential screening-selected gene aberrant in neuroblastoma; neuroblastoma candidate region; suppression of tumorigenicity 1; neuroblastoma sup; neuroblastoma; suppression of tumorigenicity 1
 Product Gene Name   

NBL1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS927613 Testing Data    Testing Data PDF
 OMIM    600613
 3D Structure    ModBase 3D Structure for P41271
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NBL1. No significant cross-reactivity or interference between human NBL1 and analogues was observed.
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 Samples    serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    34.4 pg/ml -2200 pg/ml
 Sensitivity    <8.6 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NBL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NBL1 purchase    MBS927613 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neuroblastoma, suppression of tumorigenicity 1 (NBL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 34.4 pg/ml -2200 pg/ml in biological research samples containing NBL1, with an estimated sensitivity of <8.6 pg/ml. The ELISA analytical biochemical technique of the MBS927613 kit is based on NBL1 antibody-NBL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NBL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NBL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NBL1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NBL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NBL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NBL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NBL1 elisa kit    NBL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS927613. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NBL1. It may not necessarily be applicable to this product.
 NCBI GI #    323276675
 NCBI GeneID    4681
 NCBI Accession #    NP_001191013.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001204084.2 [Other Products]
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 UniProt Primary Accession #    P41271 [Other Products]
 UniProt Secondary Accession #    Q5TGZ2; Q5U0N4; Q96L68; A3KFI7 [Other Products]
 UniProt Related Accession #    P41271 [Other Products]
 Molecular Weight    19,408 Da
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 NCBI Official Full Name    neuroblastoma suppressor of tumorigenicity 1 isoform 2
 NCBI Official Synonym Full Names    neuroblastoma 1, DAN family BMP antagonist
 NCBI Official Symbol    NBL1 [Similar Products]
 NCBI Official Synonym Symbols   
NB; DAN; NO3; DAND1; D1S1733E
[Similar Products]
 NCBI Protein Information    neuroblastoma suppressor of tumorigenicity 1; DAN domain family member 1; differential screening-selected gene aberrant in neuroblastoma; neuroblastoma candidate region, suppression of tumorigenicity 1
 UniProt Protein Name    Neuroblastoma suppressor of tumorigenicity 1
 UniProt Synonym Protein Names   
DAN domain family member 1; Protein N03; Zinc finger protein DAN
 Protein Family    N-terminal-borealin-like protein
 UniProt Gene Name    NBL1 [Similar Products]
 UniProt Synonym Gene Names    DAN; DAND1 [Similar Products]
 UniProt Entry Name    NBL1_HUMAN
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 NCBI Summary for NBL1    This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]
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 UniProt Comments for NBL1    NBL1 iso2: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Tumor suppressor; Secreted

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: extracellular space

Molecular Function: morphogen activity; protein homodimerization activity

Biological Process: nervous system development; determination of dorsal identity; positive regulation of neuron differentiation; neurite morphogenesis; negative regulation of BMP signaling pathway
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 Research Articles on NBL1    1. NBL1 is a secreted protein that is highly restricted to the prostate. Underexpression of NBL1 correlated with prostate cancer progression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NBL1 elisa kitOrgans/Tissues associated with NBL1 elisa kit
 Reference Product  PubMed Publications
 UBQLN1 elisa kit  >2 publications with NBL1 and UBQLN1
 BAG6 elisa kit  >1 publications with NBL1 and BAG6
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >22 publications with NBL1 and Nerve
 Bone Antibodies  >16 publications with NBL1 and Bone
 Embryonic Tissue Antibodies  >5 publications with NBL1 and Embryonic Tissue
 Lung Antibodies  >5 publications with NBL1 and Lung
 Brain Antibodies  >4 publications with NBL1 and Brain
 Eye Antibodies  >3 publications with NBL1 and Eye
 Ganglia Antibodies  >3 publications with NBL1 and Ganglia
 Ovary Antibodies  >2 publications with NBL1 and Ovary
 Prostate Antibodies  >2 publications with NBL1 and Prostate
 Connective Tissue Antibodies  >1 publications with NBL1 and Connective Tissue
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