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NID1 elisa kit :: Human nidogen 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704872 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

nidogen 1 (NID1), ELISA Kit

★Popular Item★
 Also Known As   

Human NiDogen 1, NID1 ELISA Kit

 Product Synonym Names    Human Nidogen 1 (NID1) ELISA Kit; NID; enactin; entactin; nidogen 1
 Product Gene Name   

NID1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704872 Testing Data    Testing Data PDF
 OMIM    131390
 3D Structure    ModBase 3D Structure for P14543
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NID 1. No significant cross-reactivity or interference between human NID 1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates
 Assay Type    Sandwich
 Detection Range    0.312 ng/ml -20 ng/ml.
 Sensitivity    The minimum detectable dose of human NID 1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NID1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NID1 purchase    MBS704872 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nidogen 1 (NID1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml -20 ng/ml in biological research samples containing NID1, with an estimated sensitivity of The minimum detectable dose of human NID 1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS704872 kit is based on NID1 antibody-NID1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NID1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NID1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NID1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NID 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NID 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NID 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NID 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NID1 elisa kit    NID1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704872. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NID1. It may not necessarily be applicable to this product.
 NCBI GI #    115298674
 NCBI GeneID    4811
 NCBI Accession #    NP_002499.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002508.2 [Other Products]
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 UniProt Primary Accession #    P14543 [Other Products]
 UniProt Secondary Accession #    Q14942; Q59FL2; Q5TAF2; Q5TAF3; Q86XD7 [Other Products]
 UniProt Related Accession #    P14543 [Other Products]
 Molecular Weight    136,377 Da
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 NCBI Official Full Name    nidogen-1
 NCBI Official Synonym Full Names    nidogen 1
 NCBI Official Symbol    NID1 [Similar Products]
 NCBI Official Synonym Symbols   
NID
[Similar Products]
 NCBI Protein Information    nidogen-1; NID-1; enactin; entactin
 UniProt Protein Name    Nidogen-1
 UniProt Synonym Protein Names   
Entactin
 Protein Family    Nidogen
 UniProt Gene Name    NID1 [Similar Products]
 UniProt Synonym Gene Names    NID; NID-1 [Similar Products]
 UniProt Entry Name    NID1_HUMAN
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 NCBI Summary for NID1    This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NID1    Function: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
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 Research Articles on NID1    1. The cleavage of nidogen-1 and -2 is partially inhibited in human tumor samples.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NID1 elisa kitPathways associated with NID1 elisa kit
 Reference Product  PubMed Publications
 COL4A1 elisa kit  >1 publications with NID1 and COL4A1
 Products by Pathway  Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Laminin Interactions Pathway antibodies  Laminin Interactions Pathway Diagram
Diseases associated with NID1 elisa kitOrgans/Tissues associated with NID1 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with NID1 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >2 publications with NID1 and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >2 publications with NID1 and Adenocarcinoma
 Necrosis Antibodies  >2 publications with NID1 and Necrosis
 Nervous System Diseases Antibodies  >1 publications with NID1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >1 publications with NID1 and Lung Neoplasms
 Liver Neoplasms Antibodies  >1 publications with NID1 and Liver Neoplasms
 Ovarian Neoplasms Antibodies  >1 publications with NID1 and Ovarian Neoplasms
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with NID1 and Prenatal Exposure Delayed Effects
 Neural Tube Defects Antibodies  >1 publications with NID1 and Neural Tube Defects
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >2 publications with NID1 and Heart
 Brain Antibodies  >2 publications with NID1 and Brain
 Stomach Antibodies  >1 publications with NID1 and Stomach
 Kidney Antibodies  >1 publications with NID1 and Kidney
 Spleen Antibodies  >1 publications with NID1 and Spleen
 Skin Antibodies  >1 publications with NID1 and Skin
 Lung Antibodies  >1 publications with NID1 and Lung
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