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NOS1 elisa kit :: Human nitric oxide synthase 1 (neuronal) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700071
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

nitric oxide synthase 1 (neuronal) (NOS1), ELISA Kit

★Popular Item★
 Also Known As   

Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit

 Product Synonym Names    Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit; IHPS1; NOS; nNOS; nitric oxide synthase 1; neuronal; nitric oxide synthase 1 (neuronal)
 Product Gene Name   

NOS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700071 Testing Data    Testing Data PDF
 OMIM    163731
 3D Structure    ModBase 3D Structure for P29475
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human NOS1. No significant cross-reactivity or interference between Human NOS1 and analogues was observed.
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 Samples    Serum, plasma and cell culture supernates
 Detection Range    0.156 IU/ml-10 IU/ml
 Sensitivity    0.039 IU/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NOS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NOS1 purchase    MBS700071 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nitric oxide synthase 1 (neuronal) (NOS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 IU/ml-10 IU/ml in biological research samples containing NOS1, with an estimated sensitivity of 0.039 IU/ml. The ELISA analytical biochemical technique of the MBS700071 kit is based on NOS1 antibody-NOS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NOS1 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to nNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for nNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain nNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of nNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NOS1 elisa kit    NOS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700071. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NOS1. It may not necessarily be applicable to this product.
 NCBI GI #    10835173
 NCBI GeneID    4842
 NCBI Accession #    NP_000611.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000620.4 [Other Products]
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 UniProt Primary Accession #    P29475 [Other Products]
 UniProt Secondary Accession #    O75713; E9PH30 [Other Products]
 UniProt Related Accession #    P29475 [Other Products]
 Molecular Weight    160,970 Da
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 NCBI Official Full Name    nitric oxide synthase, brain isoform 1
 NCBI Official Synonym Full Names    nitric oxide synthase 1 (neuronal)
 NCBI Official Symbol    NOS1 [Similar Products]
 NCBI Official Synonym Symbols   
NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
[Similar Products]
 NCBI Protein Information    nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1
 UniProt Protein Name    Nitric oxide synthase, brain
 UniProt Synonym Protein Names   
Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS
 Protein Family    Nitric oxide synthase
 UniProt Gene Name    NOS1 [Similar Products]
 UniProt Synonym Gene Names    N-NOS; nNOS [Similar Products]
 UniProt Entry Name    NOS1_HUMAN
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 NCBI Summary for NOS1    The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]
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 UniProt Comments for NOS1    Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group.

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.
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 Research Articles on NOS1    1. Genetic or pharmacologic inhibition of NOS1 reduced the growth of CXCL14-expressing fibroblasts.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NOS1 elisa kitPathways associated with NOS1 elisa kit
 Reference Product  PubMed Publications
 CAV3 elisa kit  >7 publications with NOS1 and CAV3
 ARG1 elisa kit  >6 publications with NOS1 and ARG1
 UBC elisa kit  >3 publications with NOS1 and UBC
 DLG4 elisa kit  >2 publications with NOS1 and DLG4
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
Diseases associated with NOS1 elisa kitOrgans/Tissues associated with NOS1 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >489 publications with NOS1 and Nervous System Diseases
 Brain Diseases Antibodies  >261 publications with NOS1 and Brain Diseases
 Disease Models, Animal Antibodies  >230 publications with NOS1 and Disease Models, Animal
 Hypertension Antibodies  >160 publications with NOS1 and Hypertension
 Mental Disorders Antibodies  >149 publications with NOS1 and Mental Disorders
 Inflammation Antibodies  >129 publications with NOS1 and Inflammation
 Brain Ischemia Antibodies  >105 publications with NOS1 and Brain Ischemia
 Heart Diseases Antibodies  >94 publications with NOS1 and Heart Diseases
 Pain Antibodies  >92 publications with NOS1 and Pain
 Kidney Diseases Antibodies  >60 publications with NOS1 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with NOS1 and Bone
 Connective Tissue Antibodies  >4 publications with NOS1 and Connective Tissue
 Heart Antibodies  >3 publications with NOS1 and Heart
 Muscle Antibodies  >3 publications with NOS1 and Muscle
 Brain Antibodies  >3 publications with NOS1 and Brain
 Vascular Antibodies  >2 publications with NOS1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with NOS1 and Embryonic Tissue
 Ear Antibodies  >1 publications with NOS1 and Ear
 Kidney Antibodies  >1 publications with NOS1 and Kidney
 Umbilical Cord Antibodies  >1 publications with NOS1 and Umbilical Cord
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