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NOG elisa kit :: Human noggin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705869
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

noggin, ELISA Kit

★Popular Item★
 Also Known As   

Human Noggin, NOG ELISA Kit

 Product Synonym Names    Human Noggin (NOG) ELISA kit; SYM1; SYNS1; symphalangism 1 (proximal) ; noggin
 Product Gene Name   

NOG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705869 Testing Data    Testing Data PDF
 OMIM    gene 611377
 3D Structure    ModBase 3D Structure for Q13253
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NOG. No significant cross-reactivity or interference between human NOG and analogues was observed.
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 Samples    Serum, plasma
 Assay Type    Sandwich
 Detection Range    1.56 ng/ml-100 ng/ml.
 Sensitivity    The minimum detectable dose of human NOG is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NOG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NOG purchase    MBS705869 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the noggin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 ng/ml-100 ng/ml in biological research samples containing NOG, with an estimated sensitivity of The minimum detectable dose of human NOG is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS705869 kit is based on NOG antibody-NOG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NOG elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOG bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NOG elisa kit    NOG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705869. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NOG. It may not necessarily be applicable to this product.
 NCBI GI #    4885523
 NCBI GeneID    9241
 NCBI Accession #    NP_005441.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005450.4 [Other Products]
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 UniProt Primary Accession #    Q13253 [Other Products]
 UniProt Related Accession #    Q13253 [Other Products]
 Molecular Weight    25,774 Da
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 NCBI Official Full Name    noggin
 NCBI Official Synonym Full Names    noggin
 NCBI Official Symbol    NOG [Similar Products]
 NCBI Official Synonym Symbols   
SYM1; SYNS1
[Similar Products]
 NCBI Protein Information    noggin; symphalangism 1 (proximal)
 UniProt Protein Name    Noggin
 Protein Family    Noggin
 UniProt Gene Name    NOG [Similar Products]
 UniProt Entry Name    NOGG_HUMAN
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 NCBI Summary for NOG    The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NOG    NOG: Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in NOG are a cause of symphalangism proximal syndrome (SYM1). SYM1 is characterized by the hereditary absence of the proximal interphalangeal (PIP) joints (Cushing symphalangism). Severity of PIP joint involvement diminishes towards the radial side. Distal interphalangeal joints are less frequently involved and metacarpophalangeal joints are rarely affected whereas carpal bone malformation and fusion are common. In the lower extremities, tarsal bone coalition is common. Conducive hearing loss is seen and is due to fusion of the stapes to the petrous part of the temporal bone. Defects in NOG are the cause of multiple synostoses syndrome type 1 (SYNS1); also known as synostoses, multiple, with brachydactyly/symphalangism-brachydactyly syndrome. SYNS1 is characterized by tubular-shaped (hemicylindrical) nose with lack of alar flare, otosclerotic deafness, and multiple progressive joint fusions commencing in the hand. The joint fusions are progressive, commencing in the fifth proximal interphalangeal joint in early childhood (or at birth in some individuals) and progressing in an ulnar-to-radial and proximal- to-distal direction. With increasing age, ankylosis of other joints, including the cervical vertebrae, hips, and humeroradial joints, develop. Defects in NOG are the cause of tarsal-carpal coalition syndrome (TCC). TCC is an autosomal dominant disorder characterized by fusion of the carpals, tarsals and phalanges, short first metacarpals causing brachydactyly, and humeroradial fusion. TCC is allelic to SYM1, and different mutations in NOG can result in either TCC or SYM1 in different families. Defects in NOG are a cause of stapes ankylosis with broad thumb and toes (SABTS); also known as Teunissen- Cremers syndrome. SABTS is a congenital autosomal dominant disorder that includes hyperopia, a hemicylindrical nose, broad thumbs, great toes, and other minor skeletal anomalies but lacked carpal and tarsal fusion and symphalangism. Defects in NOG are the cause of brachydactyly type B2 (BDB2). BDB2 is a subtype of brachydactyly characterized by hypoplasia/aplasia of distal phalanges in combination with distal symphalangism, fusion of carpal/tarsal bones, and partial cutaneous syndactyly. Belongs to the noggin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; protein homodimerization activity; cytokine binding

Biological Process: limb development; wound healing; somatic stem cell maintenance; embryonic skeletal development; motor axon guidance; middle ear morphogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; BMP signaling pathway; notochord morphogenesis; cell differentiation in hindbrain; negative regulation of cardiac muscle cell proliferation; axial mesoderm development; negative regulation of osteoblast differentiation; skeletal development; negative regulation of cell migration; nervous system development; in utero embryonic development; neural plate morphogenesis; osteoblast differentiation; mesoderm formation; dorsal/ventral pattern formation; pituitary gland development; spinal cord development; endoderm formation; cartilage development; neural tube closure; negative regulation of astrocyte differentiation; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; positive regulation of epithelial cell proliferation

Disease: Stapes Ankylosis With Broad Thumb And Toes; Symphalangism, Proximal, 1a; Brachydactyly, Type B2; Multiple Synostoses Syndrome 1; Tarsal-carpal Coalition Syndrome
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 Research Articles on NOG    1. this study proposes that the decreased binding affinity of NOG with the p.R136C mutation to HSPG leads to an excess of bone morphogenetic protein signaling and underlies the proximal symphalangism and conductive hearing loss phenotype of carriers.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NOG elisa kitPathways associated with NOG elisa kit
 Reference Product  PubMed Publications
 BMP4 elisa kit  >20 publications with NOG and BMP4
 GDF5 elisa kit  >11 publications with NOG and GDF5
 BMP2 elisa kit  >11 publications with NOG and BMP2
 BMPR1A elisa kit  >9 publications with NOG and BMPR1A
 BMPR1B elisa kit  >5 publications with NOG and BMPR1B
 BMPR2 elisa kit  >4 publications with NOG and BMPR2
 BMP7 elisa kit  >4 publications with NOG and BMP7
 BMP6 elisa kit  >2 publications with NOG and BMP6
 Products by Pathway  Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 BMP Signalling And Regulation Pathway antibodies  BMP Signalling And Regulation Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By BMP Pathway antibodies  Signaling By BMP Pathway Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
Diseases associated with NOG elisa kitOrgans/Tissues associated with NOG elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >57 publications with NOG and Disease Models, Animal
 Nervous System Diseases Antibodies  >37 publications with NOG and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >23 publications with NOG and Neoplasms, Experimental
 Liver Diseases Antibodies  >16 publications with NOG and Liver Diseases
 Inflammation Antibodies  >15 publications with NOG and Inflammation
 Neoplasm Metastasis Antibodies  >12 publications with NOG and Neoplasm Metastasis
 Liver Neoplasms Antibodies  >11 publications with NOG and Liver Neoplasms
 Cardiovascular Diseases Antibodies  >10 publications with NOG and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >9 publications with NOG and Breast Neoplasms
 NOG-Related-Symphalangism Spectrum Disorder Antibodies  >7 publications with NOG and NOG-Related-Symphalangism Spectrum Disorder
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with NOG and Bone
 Connective Tissue Antibodies  >4 publications with NOG and Connective Tissue
 Heart Antibodies  >3 publications with NOG and Heart
 Muscle Antibodies  >3 publications with NOG and Muscle
 Brain Antibodies  >3 publications with NOG and Brain
 Vascular Antibodies  >2 publications with NOG and Vascular
 Embryonic Tissue Antibodies  >2 publications with NOG and Embryonic Tissue
 Ear Antibodies  >1 publications with NOG and Ear
 Kidney Antibodies  >1 publications with NOG and Kidney
 Umbilical Cord Antibodies  >1 publications with NOG and Umbilical Cord
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