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NCAPD3 elisa kit :: Human non-SMC condensin II complex, subunit D3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS922529
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

non-SMC condensin II complex, subunit D3 (NCAPD3), ELISA Kit

★Popular Item★
 Also Known As   

Human Condensin-2 complex subunit D3, NCAPD3 ELISA Kit

 Product Synonym Names    Human Condensin-2 complex subunit D3 (NCAPD3) ELISA kit; CAP-D3; FLJ42888; KIAA0056; MGC104671; hCAP-D3; hHCP-6; hcp-6; ; non-SMC condensin II complex; subunit D3
 Product Gene Name   

NCAPD3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS922529 Testing Data    Testing Data PDF
 OMIM    609276
 3D Structure    ModBase 3D Structure for P42695
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NCAPD3. No significant cross-reactivity or interference between human NCAPD3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml -1600 pg/ml.
 Sensitivity    The minimum detectable dose of human NCAPD3 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NCAPD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NCAPD3 purchase    MBS922529 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the non-SMC condensin II complex, subunit D3 (NCAPD3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing NCAPD3, with an estimated sensitivity of The minimum detectable dose of human NCAPD3 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS922529 kit is based on NCAPD3 antibody-NCAPD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NCAPD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NCAPD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NCAPD3 elisa kit

   Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NCAPD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NCAPD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCAPD3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCAPD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NCAPD3 elisa kit    NCAPD3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS922529. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NCAPD3. It may not necessarily be applicable to this product.
 NCBI GI #    45356151
 NCBI GeneID    23310
 NCBI Accession #    NP_056076.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_015261.2 [Other Products]
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 UniProt Primary Accession #    P42695 [Other Products]
 UniProt Secondary Accession #    Q4KMQ9; A6NFS2 [Other Products]
 UniProt Related Accession #    P42695 [Other Products]
 Molecular Weight    168,891 Da
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 NCBI Official Full Name    condensin-2 complex subunit D3
 NCBI Official Synonym Full Names    non-SMC condensin II complex, subunit D3
 NCBI Official Symbol    NCAPD3 [Similar Products]
 NCBI Official Synonym Symbols   
CAPD3; hcp-6; CAP-D3; hHCP-6; hCAP-D3
[Similar Products]
 NCBI Protein Information    condensin-2 complex subunit D3
 UniProt Protein Name    Condensin-2 complex subunit D3
 UniProt Synonym Protein Names   
Non-SMC condensin II complex subunit D3; hCAP-D3
 Protein Family    Condensin-2 complex
 UniProt Gene Name    NCAPD3 [Similar Products]
 UniProt Synonym Gene Names    CAPD3; KIAA0056; hCAP-D3 [Similar Products]
 UniProt Entry Name    CNDD3_HUMAN
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 NCBI Summary for NCAPD3    Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for NCAPD3    NCAPD3: Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: nucleoplasm; germinal vesicle; membrane

Molecular Function: protein binding; methylated histone residue binding

Biological Process: mitotic chromosome condensation; cell division; mitotic cell cycle
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 Research Articles on NCAPD3    1. The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with NCAPD3 elisa kitOrgans/Tissues associated with NCAPD3 elisa kit
 Disease Name  Pubmed Publications
 Birth Weight Antibodies  >1 publications with NCAPD3 and Birth Weight
 Mental Disorders Diagnosed in Childhood Antibodies  >1 publications with NCAPD3 and Mental Disorders Diagnosed in Childhood
 Craniofacial Abnormalities Antibodies  >1 publications with NCAPD3 and Craniofacial Abnormalities
 Chromosome Aberrations Antibodies  >1 publications with NCAPD3 and Chromosome Aberrations
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with NCAPD3 and Heart
 Brain Antibodies  >1 publications with NCAPD3 and Brain
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