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PPIA elisa kit :: Human peptidylprolyl isomerase A (cyclophilin A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702050
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

peptidylprolyl isomerase A (cyclophilin A) (PPIA), ELISA Kit

 Also Known As   

Human cyclophilin A, PPIA/CYPA ELISA Kit

 Product Synonym Names    Human cyclophilin A (PPIA/CYPA) ELISA Kit; CYPA; CYPH; MGC117158; MGC12404; MGC23397; PPIase A; T cell cyclophilin; cyclosporin A-binding protein; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A; rotamase A; peptidylprolyl isomerase A (cyclophilin A)
 Product Gene Name   

PPIA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702050 Testing Data    Testing Data PDF
 OMIM    123840
 3D Structure    ModBase 3D Structure for P62937
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PPIA. No significant cross-reactivity or interference between Human PPIA and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Detection Range    3.12 ng/ml-200 ng/ml
 Sensitivity    0.78 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Immunity
 Protein Biological Process 3    Host-virus interaction
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PPIA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PPIA purchase    MBS702050 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the peptidylprolyl isomerase A (cyclophilin A) (PPIA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 ng/ml-200 ng/ml in biological research samples containing PPIA, with an estimated sensitivity of 0.78 ng/ml. The ELISA analytical biochemical technique of the MBS702050 kit is based on PPIA antibody-PPIA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPIA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPIA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PPIA elisa kit

   Introduction: Cyclophilins, abbreviated Cyp, are proteins that bind to cyclosporine (cyclosporin A), an immunosuppressant which is usually used to suppress rejection after internal organ transplants. These proteins have peptidyl prolyl isomerase activity, which catalyzes the isomerization of peptide bonds from trans form to cis form at proline residues and facilitates protein folding. Cyclophilin A (CyPA) is an abundant intracellular protein that is considered to be the main target of the immunosuppressive drug cyclosporine A. Human Cyclophilin A exists in cytosol, has a beta barrel structure with two alpha helices and a beta-sheet. Other cyclophilins have similar structures to cyclophilin A. The cyclosporin A-cyclophilin A complex inhibits a calcium/calmodulin-dependent phosphatase, calcineurin, the inhibition of which is thought to suppress organ rejection by halting the production of the pro-inflammatory molecules TNF alpha and interleukin 2.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CyPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for CyPA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CyPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CyPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PPIA elisa kit    PPIA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702050. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PPIA. It may not necessarily be applicable to this product.
 NCBI GI #    10863927
 NCBI GeneID    5478
 NCBI Accession #    NP_066953.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021130.4 [Other Products]
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 UniProt Primary Accession #    P62937 [Other Products]
 UniProt Secondary Accession #    P05092; Q3KQW3; Q6IBU5; Q96IX3; Q9BRU4; Q9BTY9; Q9UC61; A8K220 [Other Products]
 UniProt Related Accession #    P62937 [Other Products]
 Molecular Weight    18,012 Da
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 NCBI Official Full Name    peptidyl-prolyl cis-trans isomerase A isoform 1
 NCBI Official Synonym Full Names    peptidylprolyl isomerase A (cyclophilin A)
 NCBI Official Symbol    PPIA [Similar Products]
 NCBI Official Synonym Symbols   
CYPA; CYPH; HEL-S-69p
[Similar Products]
 NCBI Protein Information    peptidyl-prolyl cis-trans isomerase A; PPIase A; rotamase A; T cell cyclophilin; cyclosporin A-binding protein; epididymis secretory sperm binding protein Li 69p
 UniProt Protein Name    Peptidyl-prolyl cis-trans isomerase A
 UniProt Synonym Protein Names   
Cyclophilin A; Cyclosporin A-binding protein; Rotamase ACleaved into the following chain:Peptidyl-prolyl cis-trans isomerase A, N-terminally processed
 Protein Family    Peptidyl-prolyl cis-trans isomerase
 UniProt Gene Name    PPIA [Similar Products]
 UniProt Synonym Gene Names    CYPA; PPIase A [Similar Products]
 UniProt Entry Name    PPIA_HUMAN
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 NCBI Summary for PPIA    This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PPIA    Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulation: Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase.
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 Research Articles on PPIA    1. Patients with type 2 diabetes have higher circulating levels of cyclophilin A than the normal population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PPIA elisa kitPathways associated with PPIA elisa kit
 Reference Product  PubMed Publications
 CD4 elisa kit  >9 publications with PPIA and CD4
 CFL1 elisa kit  >2 publications with PPIA and CFL1
 NFATC1 elisa kit  >1 publications with PPIA and NFATC1
 Products by Pathway  Pathway Diagram
 APOBEC3G Mediated Resistance To HIV-1 Infection Pathway antibodies  APOBEC3G Mediated Resistance To HIV-1 Infection Pathway Diagram
 Assembly Of The HIV Virion Pathway antibodies  Assembly Of The HIV Virion Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 Binding And Entry Of HIV Virion Pathway antibodies  Binding And Entry Of HIV Virion Pathway Diagram
 Budding And Maturation Of HIV Virion Pathway antibodies  Budding And Maturation Of HIV Virion Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
Diseases associated with PPIA elisa kitOrgans/Tissues associated with PPIA elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >16 publications with PPIA and Inflammation
 Cardiovascular Diseases Antibodies  >16 publications with PPIA and Cardiovascular Diseases
 Heart Diseases Antibodies  >13 publications with PPIA and Heart Diseases
 Necrosis Antibodies  >10 publications with PPIA and Necrosis
 Nervous System Diseases Antibodies  >8 publications with PPIA and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >7 publications with PPIA and Neoplasms, Experimental
 Brain Diseases Antibodies  >7 publications with PPIA and Brain Diseases
 Immune System Diseases Antibodies  >7 publications with PPIA and Immune System Diseases
 Gastrointestinal Diseases Antibodies  >6 publications with PPIA and Gastrointestinal Diseases
 Liver Diseases Antibodies  >6 publications with PPIA and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with PPIA and Bone
 Connective Tissue Antibodies  >4 publications with PPIA and Connective Tissue
 Heart Antibodies  >3 publications with PPIA and Heart
 Muscle Antibodies  >3 publications with PPIA and Muscle
 Brain Antibodies  >3 publications with PPIA and Brain
 Vascular Antibodies  >2 publications with PPIA and Vascular
 Embryonic Tissue Antibodies  >2 publications with PPIA and Embryonic Tissue
 Ear Antibodies  >1 publications with PPIA and Ear
 Kidney Antibodies  >1 publications with PPIA and Kidney
 Umbilical Cord Antibodies  >1 publications with PPIA and Umbilical Cord
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