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| Product Name
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peptidylprolyl isomerase A (cyclophilin A) (PPIA), ELISA Kit ★Popular Item★
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| Also Known As
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Human cyclophilin A, PPIA/CYPA ELISA Kit
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| Product Synonym Names
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Human cyclophilin A (PPIA/CYPA) ELISA Kit; CYPA; CYPH; MGC117158; MGC12404; MGC23397; PPIase A; T cell cyclophilin; cyclosporin A-binding protein; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A; rotamase A; peptidylprolyl isomerase A (cyclophilin A)
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS702050 Testing Data
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| OMIM
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123840
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| 3D Structure
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ModBase 3D Structure for P62937
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| Species Reactivity
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Human
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of Human PPIA. No significant cross-reactivity or interference between Human PPIA and analogues was observed.
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| Samples
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Serum, plasma, tissue homogenates, Cell lysates
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| Detection Range
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3.12 ng/ml-200 ng/ml
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| Sensitivity
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0.78 ng/ml
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
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| Detection Wavelength
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450 nm
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| Sample Volume
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50-100ul
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| Protein Biological Process 1
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Immunity
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| Protein Biological Process 3
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Host-virus interaction
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| Preparation and Storage
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of PPIA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for PPIA purchase
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MBS702050 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the peptidylprolyl isomerase A (cyclophilin A) (PPIA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 ng/ml-200 ng/ml in biological research samples containing PPIA, with an estimated sensitivity of 0.78 ng/ml. The ELISA analytical biochemical technique of the MBS702050 kit is based on PPIA antibody-PPIA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPIA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPIA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PPIA elisa kit
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Introduction: Cyclophilins, abbreviated Cyp, are proteins that bind to cyclosporine (cyclosporin A), an immunosuppressant which is usually used to suppress rejection after internal organ transplants. These proteins have peptidyl prolyl isomerase activity, which catalyzes the isomerization of peptide bonds from trans form to cis form at proline residues and facilitates protein folding. Cyclophilin A (CyPA) is an abundant intracellular protein that is considered to be the main target of the immunosuppressive drug cyclosporine A. Human Cyclophilin A exists in cytosol, has a beta barrel structure with two alpha helices and a beta-sheet. Other cyclophilins have similar structures to cyclophilin A. The cyclosporin A-cyclophilin A complex inhibits a calcium/calmodulin-dependent phosphatase, calcineurin, the inhibition of which is thought to suppress organ rejection by halting the production of the pro-inflammatory molecules TNF alpha and interleukin 2.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CyPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for CyPA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CyPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CyPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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| Typical Testing Data/Standard Curve (for reference only) of PPIA elisa kit
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| Sample Manual Insert of MBS702050. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for PPIA. It may not necessarily be applicable to this product. |
| NCBI GI #
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10863927
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| NCBI GeneID
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5478
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| NCBI Accession #
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NP_066953.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_021130.4
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[Other Products]
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| UniProt Primary Accession #
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P62937
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[Other Products]
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| UniProt Secondary Accession #
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P05092; Q3KQW3; Q6IBU5; Q96IX3; Q9BRU4; Q9BTY9; Q9UC61; A8K220
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[Other Products]
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| UniProt Related Accession #
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P62937
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[Other Products]
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| Molecular Weight
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18,012 Da
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| NCBI Official Full Name
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peptidyl-prolyl cis-trans isomerase A isoform 1
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| NCBI Official Synonym Full Names
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peptidylprolyl isomerase A (cyclophilin A)
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| NCBI Official Symbol
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PPIA
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[Similar Products]
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| NCBI Official Synonym Symbols
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CYPA; CYPH; HEL-S-69p
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[Similar Products]
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| NCBI Protein Information
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peptidyl-prolyl cis-trans isomerase A; PPIase A; rotamase A; T cell cyclophilin; cyclosporin A-binding protein; epididymis secretory sperm binding protein Li 69p
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| UniProt Protein Name
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Peptidyl-prolyl cis-trans isomerase A
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| UniProt Synonym Protein Names
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Cyclophilin A; Cyclosporin A-binding protein; Rotamase ACleaved into the following chain:Peptidyl-prolyl cis-trans isomerase A, N-terminally processed
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| Protein Family
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Peptidyl-prolyl cis-trans isomerase
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| UniProt Gene Name
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PPIA
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[Similar Products]
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| UniProt Synonym Gene Names
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CYPA; PPIase A
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[Similar Products]
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| UniProt Entry Name
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PPIA_HUMAN
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| NCBI Summary for PPIA
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This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]
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| UniProt Comments for PPIA
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Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation: Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase.
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| Research Articles on PPIA
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1. Patients with type 2 diabetes have higher circulating levels of cyclophilin A than the normal population.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with PPIA elisa kit | Pathways associated with PPIA elisa kit |
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| Diseases associated with PPIA elisa kit | Organs/Tissues associated with PPIA elisa kit |
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ELISA Kit 
