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POSTN elisa kit :: Human periostin/osteoblast specific factor 2 (POSTN) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705827
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

periostin/osteoblast specific factor 2 (POSTN), ELISA Kit

★Popular Item★
 Also Known As   

Human periostin/osteoblast specific factor 2, POSTN ELISA Kit

 Product Synonym Names    Human periostin/osteoblast specific factor 2 (POSTN) ELISA kit; MGC119510; MGC119511; OSF-2; OSF2; PDLPOSTN; PN; RP11-412K4.1; periostin; osteoblast specific factor 2 (fasciclin I-like) ; periodontal ligament-specific periostin; periostin isoform thy2; periostin iso; periostin/osteoblast specific factor 2 (POSTN)
 Product Gene Name   

POSTN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705827 Testing Data    Testing Data PDF
 OMIM    608777
 3D Structure    ModBase 3D Structure for Q15063
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human POSTN. No significant cross-reactivity or interference between human POSTN and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    6.25 ng/ml -400 ng/ml.
 Sensitivity    The minimum detectable dose of human POSTN is typically less than 1.56 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of POSTN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for POSTN purchase    MBS705827 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the periostin/osteoblast specific factor 2 (POSTN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 ng/ml -400 ng/ml in biological research samples containing POSTN, with an estimated sensitivity of The minimum detectable dose of human POSTN is typically less than 1.56 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS705827 kit is based on POSTN antibody-POSTN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POSTN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POSTN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for POSTN elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for POSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POSTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of POSTN elisa kit    POSTN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705827. Click to request current manual
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NCBI/Uniprot data below describe general gene information for POSTN. It may not necessarily be applicable to this product.
 NCBI GI #    209862911
 NCBI GeneID    10631
 NCBI Accession #    NP_001129406.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001135934.1 [Other Products]
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 UniProt Primary Accession #    Q15063 [Other Products]
 UniProt Secondary Accession #    Q15064; Q29XZ0; Q3KPJ5; Q5VSY5; Q8IZF9; B1ALD8; C0IMJ1; C0IMJ2; C0IMJ4; D2KRH7; F5H628 [Other Products]
 UniProt Related Accession #    Q15063 [Other Products]
 Molecular Weight    93,314 Da
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 NCBI Official Full Name    periostin isoform 2
 NCBI Official Synonym Full Names    periostin, osteoblast specific factor
 NCBI Official Symbol    POSTN [Similar Products]
 NCBI Official Synonym Symbols   
PN; OSF2; OSF-2; PDLPOSTN; periostin
[Similar Products]
 NCBI Protein Information    periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like)
 UniProt Protein Name    Periostin
 UniProt Synonym Protein Names   
Osteoblast-specific factor 2; OSF-2
 Protein Family    Periostin
 UniProt Gene Name    POSTN [Similar Products]
 UniProt Synonym Gene Names    OSF2; PN; OSF-2 [Similar Products]
 UniProt Entry Name    POSTN_HUMAN
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 NCBI Summary for POSTN    This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
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 UniProt Comments for POSTN    Function: Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.2 PublicationsManual assertion based on experiment in:Ref.2
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 Research Articles on POSTN    1. Periostin plays an important role for proper collagen assembly and homeostasis. During skin aging periostin expression decreases and contributes to the phenotype of aged skin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with POSTN elisa kitPathways associated with POSTN elisa kit
 Reference Product  PubMed Publications
 TGFB1 elisa kit  >30 publications with POSTN and TGFB1
 COL1A1 elisa kit  >7 publications with POSTN and COL1A1
 COL3A1 elisa kit  >7 publications with POSTN and COL3A1
 DCN elisa kit  >6 publications with POSTN and DCN
 COL1A2 elisa kit  >5 publications with POSTN and COL1A2
 FN1 elisa kit  >5 publications with POSTN and FN1
 CLCA1 elisa kit  >4 publications with POSTN and CLCA1
 SERPINB2 elisa kit  >4 publications with POSTN and SERPINB2
 COL5A2 elisa kit  >3 publications with POSTN and COL5A2
 LUM elisa kit  >3 publications with POSTN and LUM
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Diseases associated with POSTN elisa kitOrgans/Tissues associated with POSTN elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >59 publications with POSTN and Fibrosis
 Inflammation Antibodies  >43 publications with POSTN and Inflammation
 Neoplasm Metastasis Antibodies  >37 publications with POSTN and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >36 publications with POSTN and Disease Models, Animal
 Adenocarcinoma Antibodies  >29 publications with POSTN and Adenocarcinoma
 Lung Neoplasms Antibodies  >25 publications with POSTN and Lung Neoplasms
 Breast Neoplasms Antibodies  >25 publications with POSTN and Breast Neoplasms
 Myocardial Infarction Antibodies  >18 publications with POSTN and Myocardial Infarction
 Heart Failure Antibodies  >18 publications with POSTN and Heart Failure
 Nervous System Diseases Antibodies  >14 publications with POSTN and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with POSTN and Bone
 Connective Tissue Antibodies  >4 publications with POSTN and Connective Tissue
 Heart Antibodies  >3 publications with POSTN and Heart
 Muscle Antibodies  >3 publications with POSTN and Muscle
 Brain Antibodies  >3 publications with POSTN and Brain
 Vascular Antibodies  >2 publications with POSTN and Vascular
 Embryonic Tissue Antibodies  >2 publications with POSTN and Embryonic Tissue
 Ear Antibodies  >1 publications with POSTN and Ear
 Kidney Antibodies  >1 publications with POSTN and Kidney
 Umbilical Cord Antibodies  >1 publications with POSTN and Umbilical Cord
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