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PEMT elisa kit :: Human phosphatidylethanolamine N-methyltransferase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS924990
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
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 Product Name   

phosphatidylethanolamine N-methyltransferase (PEMT), ELISA Kit

★Popular Item★
 Also Known As   

Human Phosphatidylethanolamine N-methyltransferase, PEMT ELISA Kit

 Product Synonym Names    Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA kit; MGC2483; PEAMT; PEMPT; PEMT2; PNMT; OTTHUMP00000065537; phosphatidylethanolamine N-methyltransferase
 Product Gene Name   

PEMT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS924990 Testing Data    Testing Data PDF
 OMIM    602391
 3D Structure    ModBase 3D Structure for Q9UBM1
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PEMT. No significant cross-reactivity or interference between human PEMT and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    25 pg/ml -1600 pg/ml
 Sensitivity    Typically less than 6.25 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PEMT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PEMT purchase    MBS924990 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing PEMT, with an estimated sensitivity of Typically less than 6.25 pg/ml. The ELISA analytical biochemical technique of the MBS924990 kit is based on PEMT antibody-PEMT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PEMT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PEMT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PEMT elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PEMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PEMT elisa kit    PEMT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS924990. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PEMT. It may not necessarily be applicable to this product.
 NCBI GI #    388890235
 NCBI GeneID    10400
 NCBI Accession #    NP_001254480.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001267551.1 [Other Products]
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 UniProt Primary Accession #    Q9UBM1 [Other Products]
 UniProt Secondary Accession #    Q6IAQ5; Q86VL3; Q9BW86; Q9UHY6; Q9Y6V9; A8MZ66; B4DY41; D3DXC3 [Other Products]
 UniProt Related Accession #    Q9UBM1 [Other Products]
 Molecular Weight    22,134 Da
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 NCBI Official Full Name    phosphatidylethanolamine N-methyltransferase isoform 4
 NCBI Official Synonym Full Names    phosphatidylethanolamine N-methyltransferase
 NCBI Official Symbol    PEMT [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    phosphatidylethanolamine N-methyltransferase
 UniProt Protein Name    Phosphatidylethanolamine N-methyltransferase
 UniProt Synonym Protein Names   
 Protein Family    Phosphatidylethanolamine N-methyltransferase
 UniProt Gene Name    PEMT [Similar Products]
 UniProt Synonym Gene Names    PEMPT; PNMT; PEAMT; PEMT [Similar Products]
 UniProt Entry Name    PEMT_HUMAN
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 NCBI Summary for PEMT    Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]
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 UniProt Comments for PEMT    PEMT iso2: Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC). Belongs to the PEMT/PEM2 methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Mitochondrial; Methyltransferase; Lipid Metabolism - glycerophospholipid; EC; Membrane protein, integral; Endoplasmic reticulum; EC

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: endoplasmic reticulum membrane; mitochondrial membrane; integral to membrane

Molecular Function: phosphatidyl-N-methylethanolamine N-methyltransferase activity; phosphatidylethanolamine N-methyltransferase activity

Biological Process: methylation; cell proliferation; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process
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 Research Articles on PEMT    1. The GG genotype of the PEMT G774C polymorphism, higher levels of serum homocysteine and lower levels of serum betaine are associated with an increased risk of microangiopathy in patients with diabetes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PEMT elisa kitDiseases associated with PEMT elisa kit
 Products by Pathway  Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >39 publications with PEMT and Liver Diseases
 Fatty Liver Antibodies  >26 publications with PEMT and Fatty Liver
 Neoplasms Antibodies  >17 publications with PEMT and Neoplasms
 Liver Neoplasms Antibodies  >14 publications with PEMT and Liver Neoplasms
 Liver Neoplasms, Experimental Antibodies  >11 publications with PEMT and Liver Neoplasms, Experimental
 Brain Diseases Antibodies  >4 publications with PEMT and Brain Diseases
 Congenital Abnormalities Antibodies  >4 publications with PEMT and Congenital Abnormalities
 Pain Antibodies  >4 publications with PEMT and Pain
 Neurodegenerative Diseases Antibodies  >3 publications with PEMT and Neurodegenerative Diseases
 Weight Gain Antibodies  >3 publications with PEMT and Weight Gain
Organs/Tissues associated with PEMT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >88 publications with PEMT and Liver
 Blood Antibodies  >29 publications with PEMT and Blood
 Brain Antibodies  >21 publications with PEMT and Brain
 Muscle Antibodies  >5 publications with PEMT and Muscle
 Intestine Antibodies  >5 publications with PEMT and Intestine
 Lung Antibodies  >4 publications with PEMT and Lung
 Heart Antibodies  >4 publications with PEMT and Heart
 Ovary Antibodies  >3 publications with PEMT and Ovary
 Kidney Antibodies  >3 publications with PEMT and Kidney
 Adrenal Gland Antibodies  >2 publications with PEMT and Adrenal Gland
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