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PGM1 elisa kit :: Human phosphoglucomutase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS910386
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

phosphoglucomutase 1 (PGM1), ELISA Kit

★Popular Item★
 Also Known As   

Human Phosphoglucomutase-1, PGM1 ELISA Kit

 Product Synonym Names    Human Phosphoglucomutase-1 (PGM1) ELISA kit; GSD14; ; phosphoglucomutase 1
 Product Gene Name   

PGM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS910386 Testing Data    Testing Data PDF
 OMIM    614921
 3D Structure    ModBase 3D Structure for P36871
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PGM1. No significant cross-reactivity or interference between Human PGM1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    23.5 pg/ml-1500 pg/ml
 Sensitivity    5.8 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 2    glyconeogenesis and glycometabolism
 Protein Biological Process 3    Carbohydrate metabolism
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PGM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PGM1 purchase    MBS910386 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phosphoglucomutase 1 (PGM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.5 pg/ml-1500 pg/ml in biological research samples containing PGM1, with an estimated sensitivity of 5.8 pg/ml. The ELISA analytical biochemical technique of the MBS910386 kit is based on PGM1 antibody-PGM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PGM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PGM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PGM1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PGM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PGM1 elisa kit    PGM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS910386. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PGM1. It may not necessarily be applicable to this product.
 NCBI GI #    290463102
 NCBI GeneID    5236
 NCBI Accession #    NP_001166289.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001172818.1 [Other Products]
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 UniProt Primary Accession #    P36871 [Other Products]
 UniProt Secondary Accession #    Q16105; Q16106; Q5BKZ9; Q6NW22; Q86U74; Q96J40; Q9NTY4; B2R5N9; B4DPV0 [Other Products]
 UniProt Related Accession #    P36871 [Other Products]
 Molecular Weight    61,449 Da
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 NCBI Official Full Name    phosphoglucomutase-1 isoform 2
 NCBI Official Synonym Full Names    phosphoglucomutase 1
 NCBI Official Symbol    PGM1 [Similar Products]
 NCBI Official Synonym Symbols   
CDG1T; GSD14
[Similar Products]
 NCBI Protein Information    phosphoglucomutase-1; PGM 1; glucose phosphomutase 1
 UniProt Protein Name    Phosphoglucomutase-1
 UniProt Synonym Protein Names   
Glucose phosphomutase 1
 Protein Family    Phosphoglucomutase
 UniProt Gene Name    PGM1 [Similar Products]
 UniProt Synonym Gene Names    PGM 1 [Similar Products]
 UniProt Entry Name    PGM1_HUMAN
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 NCBI Summary for PGM1    The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]
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 UniProt Comments for PGM1    PGM1: This enzyme participates in both the breakdown and synthesis of glucose. Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14). A metabolic disorder resulting in a myopathy characterized by exercise-induced intolerance with episodes of rhabdomyolysis, normal elevation of lactate, and hyperammonemia on a forearm-exercise test. Belongs to the phosphohexose mutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; EC 5.4.2.2; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: cytoplasm; cytosol; actin cytoskeleton

Molecular Function: phosphoglucomutase activity; magnesium ion binding

Biological Process: galactose catabolic process; glycogen biosynthetic process; glycolysis; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

Disease: Congenital Disorder Of Glycosylation, Type It
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 Research Articles on PGM1    1. Phosphoglucomutase 1 deficiency, previously identified as a glycogenosis, is also a congenital disorder of glycosylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PGM1 elisa kitPathways associated with PGM1 elisa kit
 Reference Product  PubMed Publications
 GPI elisa kit  >12 publications with PGM1 and GPI
 GALT elisa kit  >5 publications with PGM1 and GALT
 HK1 elisa kit  >2 publications with PGM1 and HK1
 HK2 elisa kit  >1 publications with PGM1 and HK2
 PYGB elisa kit  >1 publications with PGM1 and PYGB
 PYGL elisa kit  >1 publications with PGM1 and PYGL
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 GDP-glucose Biosynthesis Pathway antibodies  GDP-glucose Biosynthesis Pathway Diagram
 Galactose Catabolism Pathway antibodies  Galactose Catabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glucuronidation Pathway antibodies  Glucuronidation Pathway Diagram
 Glycogen Metabolism Pathway antibodies  Glycogen Metabolism Pathway Diagram
Diseases associated with PGM1 elisa kitOrgans/Tissues associated with PGM1 elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >13 publications with PGM1 and Brain Diseases
 Inflammation Antibodies  >7 publications with PGM1 and Inflammation
 Anemia Antibodies  >6 publications with PGM1 and Anemia
 Edema Antibodies  >6 publications with PGM1 and Edema
 Kidney Diseases Antibodies  >6 publications with PGM1 and Kidney Diseases
 Lung Neoplasms Antibodies  >5 publications with PGM1 and Lung Neoplasms
 Necrosis Antibodies  >5 publications with PGM1 and Necrosis
 Nephritis Antibodies  >4 publications with PGM1 and Nephritis
 Proteinuria Antibodies  >3 publications with PGM1 and Proteinuria
 Disease Models, Animal Antibodies  >3 publications with PGM1 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >353 publications with PGM1 and Blood
 Muscle Antibodies  >21 publications with PGM1 and Muscle
 Skin Antibodies  >16 publications with PGM1 and Skin
 Brain Antibodies  >15 publications with PGM1 and Brain
 Liver Antibodies  >13 publications with PGM1 and Liver
 Kidney Antibodies  >12 publications with PGM1 and Kidney
 Lung Antibodies  >11 publications with PGM1 and Lung
 Placenta Antibodies  >11 publications with PGM1 and Placenta
 Heart Antibodies  >11 publications with PGM1 and Heart
 Bone Antibodies  >9 publications with PGM1 and Bone
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