Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PLD1 rightarrow Human PLD1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Annexin A3 (ANXA3) Antibody
 • FcepsilonRII-CD23 ELISA Kit
 • X-ray repair complementing defective repair in Chinese hamst... ELISA Kit
 • ApoB-100 ELISA Kit
 • SERPINA6  Antibody
 • Tet2 Antibody
 • Polycomb complex protein BMI-1 (BMI1) Recombinant Protein
 • Acetyl-CoA carboxylase 1 (Acaca) Recombinant Protein
 • Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit
 • NNT cDNA Clone
 • EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit
 • Goat IgM Immunoglobulin
 • IgA1 ELISA Kit
 • insulin-like growth factor 2 (somatomedin A) (IGF2) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PLD1 elisa kit :: Human phospholipase D1, phosphatidylcholine-specific ELISA Kit

Scan QR to view Datasheet Catalog #    MBS914961
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

phospholipase D1, phosphatidylcholine-specific (PLD1), ELISA Kit

★Popular Item★
 Also Known As   

Human Phospholipase D1, PLD1 ELISA Kit

 Product Synonym Names    Human Phospholipase D1 (PLD1) ELISA kit; choline phosphatase 1; phospholipase D1; phospholipase D1; phophatidylcholine-specific; phospholipase D1; phosphatidylcholine-specific
 Product Gene Name   

PLD1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS914961 Testing Data    Testing Data PDF
 OMIM    602382
 3D Structure    ModBase 3D Structure for Q13393
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of human PLD1. No significant cross-reactivity or interference between human PLD1 and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml -1600 pg/ml.
 Sensitivity    The minimum detectable dose of human PLD1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PLD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PLD1 purchase    MBS914961 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phospholipase D1, phosphatidylcholine-specific (PLD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing PLD1, with an estimated sensitivity of The minimum detectable dose of human PLD1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS914961 kit is based on PLD1 antibody-PLD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for PLD1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PLD1 elisa kit    PLD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS914961. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PLD1. It may not necessarily be applicable to this product.
 NCBI GI #    4505873
 NCBI GeneID    5337
 NCBI Accession #    NP_002653.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002662.4 [Other Products]
Section Bar
 UniProt Primary Accession #    Q13393 [Other Products]
 UniProt Related Accession #    Q13393 [Other Products]
 Molecular Weight    124,184 Da
Table BarTOPTable Bar
 NCBI Official Full Name    phospholipase D1 isoform a
 NCBI Official Synonym Full Names    phospholipase D1, phosphatidylcholine-specific
 NCBI Official Symbol    PLD1 [Similar Products]
 NCBI Protein Information    phospholipase D1; choline phosphatase 1; phosphatidylcholine-hydrolyzing phospholipase D1
 UniProt Protein Name    Phospholipase D1
 UniProt Synonym Protein Names   
Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
 Protein Family    Pyridoxal 4-dehydrogenase
 UniProt Gene Name    PLD1 [Similar Products]
 UniProt Synonym Gene Names    PLD 1; hPLD1 [Similar Products]
 UniProt Entry Name    PLD1_HUMAN
Section Bar
 NCBI Summary for PLD1    This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]
Section Bar
 UniProt Comments for PLD1    PLD1: a phosphatidylcholine-hydrolyzing phospholipase. Activated by ADP-ribosylated Rho and protein kinase C. Implicated as a critical step in numerous cellular pathways including membrane trafficking and the regulation polymorphonuclear leukocyte degranulation and oxidant production. May be involved in the regulation of perinuclear intravesicular membrane traffic. Four splice-variant isoforms have been described.

Protein type: Lipid Metabolism - glycerophospholipid; Phospholipase; Motility/polarity/chemotaxis; Lipid Metabolism - ether lipid; EC 3.1.4.4

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; late endosome membrane; endocytic vesicle; perinuclear region of cytoplasm; lysosomal membrane; apical plasma membrane; endosome

Molecular Function: protein binding; phospholipase D activity; phosphoinositide binding

Biological Process: defense response to Gram-positive bacterium; phosphatidylglycerol biosynthetic process; small GTPase mediated signal transduction; phospholipid metabolic process; glycerophospholipid biosynthetic process; Ras protein signal transduction; phosphatidic acid biosynthetic process; chemotaxis; lipid catabolic process; regulation of microvillus biogenesis
Table BarTOPTable Bar
 Research Articles on PLD1    1. This data indicates that CAY10593 impairs human P2X7 independently of PLD1 stimulation and highlights the importance of ensuring that compounds used in signalling studies downstream of P2X7 activation do not affect the receptor itself.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PLD1 elisa kitPathways associated with PLD1 elisa kit
 Reference Product  PubMed Publications
 RHOA elisa kit  >44 publications with PLD1 and RHOA
 PRKCA elisa kit  >38 publications with PLD1 and PRKCA
 ARF6 elisa kit  >21 publications with PLD1 and ARF6
 ARF1 elisa kit  >21 publications with PLD1 and ARF1
 CDC42 elisa kit  >14 publications with PLD1 and CDC42
 RAC1 elisa kit  >12 publications with PLD1 and RAC1
 RALA elisa kit  >11 publications with PLD1 and RALA
 EGFR elisa kit  >9 publications with PLD1 and EGFR
 CAV1 elisa kit  >5 publications with PLD1 and CAV1
 PEA15 elisa kit  >4 publications with PLD1 and PEA15
 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Arf6 Downstream Pathway antibodies  Arf6 Downstream Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 ErbB1 Downstream Signaling Pathway antibodies  ErbB1 Downstream Signaling Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
Diseases associated with PLD1 elisa kitOrgans/Tissues associated with PLD1 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >52 publications with PLD1 and Neoplasms
 Nervous System Diseases Antibodies  >19 publications with PLD1 and Nervous System Diseases
 Brain Diseases Antibodies  >16 publications with PLD1 and Brain Diseases
 Inflammation Antibodies  >15 publications with PLD1 and Inflammation
 Carcinoma Antibodies  >13 publications with PLD1 and Carcinoma
 Breast Neoplasms Antibodies  >9 publications with PLD1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >8 publications with PLD1 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >8 publications with PLD1 and Neoplasms, Experimental
 Necrosis Antibodies  >7 publications with PLD1 and Necrosis
 Adenocarcinoma Antibodies  >6 publications with PLD1 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >56 publications with PLD1 and Brain
 Blood Antibodies  >52 publications with PLD1 and Blood
 Muscle Antibodies  >39 publications with PLD1 and Muscle
 Lung Antibodies  >24 publications with PLD1 and Lung
 Vascular Antibodies  >23 publications with PLD1 and Vascular
 Kidney Antibodies  >19 publications with PLD1 and Kidney
 Heart Antibodies  >19 publications with PLD1 and Heart
 Ovary Antibodies  >15 publications with PLD1 and Ovary
 Liver Antibodies  >12 publications with PLD1 and Liver
 Eye Antibodies  >9 publications with PLD1 and Eye
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions