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POLA1 elisa kit :: Human polymerase (DNA directed) , alpha 1, catalytic subunit ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9318825 POLA1 elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

polymerase (DNA directed) , alpha 1, catalytic subunit (POLA1), ELISA Kit

 Also Known As   

Human DNA polymerase alpha Catalytic subunit, POLA1 ELISA Kit

 Product Synonym Names    Human DNA polymerase alpha catalytic subunit (POLA1) ELISA kit; DKFZp686K1672; POLA; p180; DNA-directed DNA polymerase alpha 1; polymerase (DNA-directed); alpha (70kD) ; polymerase (DNA directed); alpha 1; catalytic subunit
 Product Gene Name   

POLA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    312040
 3D Structure    ModBase 3D Structure for P09884
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 Species Reactivity    Human
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of POLA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for POLA1 purchase    MBS9318825 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the polymerase (DNA directed), alpha 1, catalytic subunit (POLA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing POLA1. The ELISA analytical biochemical technique of the MBS9318825 kit is based on POLA1 antibody-POLA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POLA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POLA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for POLA1. It may not necessarily be applicable to this product.
 NCBI GI #    106507301
 NCBI GeneID    5422
 NCBI Accession #    NP_058633.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_016937.3 [Other Products]
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 UniProt Primary Accession #    P09884 [Other Products]
 UniProt Secondary Accession #    Q86UQ7 [Other Products]
 UniProt Related Accession #    P09884 [Other Products]
 Molecular Weight    165,913 Da
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 NCBI Official Full Name    DNA polymerase alpha catalytic subunit
 NCBI Official Synonym Full Names    polymerase (DNA directed), alpha 1, catalytic subunit
 NCBI Official Symbol    POLA1 [Similar Products]
 NCBI Official Synonym Symbols   
NSX; POLA; p180
[Similar Products]
 NCBI Protein Information    DNA polymerase alpha catalytic subunit; DNA polymerase alpha p180 subunit; polymerase (DNA-directed), alpha (70kD); DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit p180; N syndrome (mental retardation, malformations, chromosome breakage)
 UniProt Protein Name    DNA polymerase alpha catalytic subunit
 UniProt Synonym Protein Names   
DNA polymerase alpha catalytic subunit p180
 Protein Family    DNA polymerase
 UniProt Gene Name    POLA1 [Similar Products]
 UniProt Synonym Gene Names    POLA [Similar Products]
 UniProt Entry Name    DPOLA_HUMAN
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 NCBI Summary for POLA1    This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]
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 UniProt Comments for POLA1    POLA: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. Belongs to the DNA polymerase type-B family.

Protein type: Nucleotide Metabolism - purine; Transferase; DNA replication; Nucleotide Metabolism - pyrimidine; DNA repair, damage; EC 2.7.7.7; Nuclear envelope

Chromosomal Location of Human Ortholog: Xp22.1-p21.3

Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; nuclear matrix; cytoplasm; nucleolus; nuclear envelope; nucleus; chromatin

Molecular Function: protein binding; DNA binding; DNA primase activity; protein heterodimerization activity; 4 iron, 4 sulfur cluster binding; metal ion binding; nucleoside binding; DNA-directed DNA polymerase activity; nucleotide binding; chromatin binding; protein kinase binding

Biological Process: viral reproduction; DNA replication initiation; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; leading strand elongation; DNA repair; lagging strand elongation; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; DNA synthesis during DNA repair; telomere maintenance via recombination; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

Disease: N Syndrome
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 Research Articles on POLA1    1. The Pol alpha-primase complex.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with POLA1 elisa kitPathways associated with POLA1 elisa kit
 Reference Product  PubMed Publications
 POLA2 elisa kit  >1 publications with POLA1 and POLA2
 Products by Pathway  Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
 DNA Polymerase Alpha / Primase Complex Pathway antibodies  DNA Polymerase Alpha / Primase Complex Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
Diseases associated with POLA1 elisa kitOrgans/Tissues associated with POLA1 elisa kit
 Disease Name  Pubmed Publications
 Drug Toxicity Antibodies  >7 publications with POLA1 and Drug Toxicity
 Movement Disorders Antibodies  >1 publications with POLA1 and Movement Disorders
 Nervous System Diseases Antibodies  >1 publications with POLA1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >1 publications with POLA1 and Lung Neoplasms
 Neoplasms Antibodies  >1 publications with POLA1 and Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with POLA1 and Blood
 Eye Antibodies  >1 publications with POLA1 and Eye
 Brain Antibodies  >1 publications with POLA1 and Brain
 Lung Antibodies  >1 publications with POLA1 and Lung
 Liver Antibodies  >1 publications with POLA1 and Liver
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