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PDCD4 elisa kit :: Human programmed cell death 4 (neoplastic transformation inhibitor) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS941857
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), ELISA Kit

★Popular Item★
 Also Known As   

Human programmed cell death 4, PDCD4 ELISA Kit

 Product Synonym Names    Human programmed cell death 4 (PDCD4) ELISA Kit; RP11-348N5.4; H731; MGC33046; MGC33047; nuclear antigen H731; programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor)
 Product Gene Name   

PDCD4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS941857 Testing Data    Testing Data PDF
 OMIM    608610
 3D Structure    ModBase 3D Structure for Q53EL6
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PDCD4. No significant cross-reactivity or interference between human PDCD4 and analogues was observed.
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 Samples    serum, plasma
 Assay Type    Quantitative Sandwich
 Detection Range    0.156 ng/ml -10 ng/ml
 Sensitivity    <0.039 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDCD4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDCD4 purchase    MBS941857 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml -10 ng/ml in biological research samples containing PDCD4, with an estimated sensitivity of <0.039 ng/ml. The ELISA analytical biochemical technique of the MBS941857 kit is based on PDCD4 antibody-PDCD4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDCD4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDCD4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PDCD4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDCD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDCD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PDCD4 elisa kit    PDCD4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS941857. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PDCD4. It may not necessarily be applicable to this product.
 NCBI GI #    21735596
 NCBI GeneID    27250
 NCBI Accession #    NP_055271.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_014456.4 [Other Products]
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 UniProt Primary Accession #    Q53EL6 [Other Products]
 UniProt Secondary Accession #    O15501; Q5VZS6; Q6PJI5; Q8TAR5; Q99834; B2RCV4; B5ME91 [Other Products]
 UniProt Related Accession #    Q53EL6 [Other Products]
 Molecular Weight    51,735 Da
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 NCBI Official Full Name    programmed cell death protein 4 isoform 1
 NCBI Official Synonym Full Names    programmed cell death 4 (neoplastic transformation inhibitor)
 NCBI Official Symbol    PDCD4 [Similar Products]
 NCBI Official Synonym Symbols   
H731
[Similar Products]
 NCBI Protein Information    programmed cell death protein 4; protein 197/15a; nuclear antigen H731; neoplastic transformation inhibitor protein
 UniProt Protein Name    Programmed cell death protein 4
 UniProt Synonym Protein Names   
Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
 Protein Family    Programmed cell death protein
 UniProt Gene Name    PDCD4 [Similar Products]
 UniProt Synonym Gene Names    H731 [Similar Products]
 UniProt Entry Name    PDCD4_HUMAN
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 NCBI Summary for PDCD4    This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
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 UniProt Comments for PDCD4    PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle
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 Research Articles on PDCD4    1. Our results help determine the significance of Pdcd4 loss in melanoma as well as its up-regulation by Akt pathway inhibitors
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDCD4 elisa kitPathways associated with PDCD4 elisa kit
 Reference Product  PubMed Publications
 RPS13 elisa kit  >1 publications with PDCD4 and RPS13
 RPL5 elisa kit  >1 publications with PDCD4 and RPL5
 EIF4G1 elisa kit  >1 publications with PDCD4 and EIF4G1
 Products by Pathway  Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram
Diseases associated with PDCD4 elisa kitOrgans/Tissues associated with PDCD4 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >148 publications with PDCD4 and Neoplasms
 Carcinoma Antibodies  >68 publications with PDCD4 and Carcinoma
 Adenocarcinoma Antibodies  >37 publications with PDCD4 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >32 publications with PDCD4 and Cell Transformation, Neoplastic
 Gastrointestinal Diseases Antibodies  >28 publications with PDCD4 and Gastrointestinal Diseases
 Neoplasm Metastasis Antibodies  >26 publications with PDCD4 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >23 publications with PDCD4 and Breast Neoplasms
 Neoplasm Invasiveness Antibodies  >22 publications with PDCD4 and Neoplasm Invasiveness
 Carcinoma, Squamous Cell Antibodies  >17 publications with PDCD4 and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >14 publications with PDCD4 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >22 publications with PDCD4 and Lung
 Blood Antibodies  >20 publications with PDCD4 and Blood
 Liver Antibodies  >16 publications with PDCD4 and Liver
 Skin Antibodies  >16 publications with PDCD4 and Skin
 Muscle Antibodies  >15 publications with PDCD4 and Muscle
 Lymph Antibodies  >11 publications with PDCD4 and Lymph
 Lymph Node Antibodies  >11 publications with PDCD4 and Lymph Node
 Vascular Antibodies  >10 publications with PDCD4 and Vascular
 Bone Antibodies  >9 publications with PDCD4 and Bone
 Brain Antibodies  >9 publications with PDCD4 and Brain
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