Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource rightarrow ELISA Kit rightarrow PROK1 rightarrow Human PROK1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
Our Best Sellers moreseparator
 • Abl interactor 1 (ABI1) Recombinant Protein
 • Met Antibody
 • Neutrophil Gelatinase Associated Lipocalin-Lipocalin-2 (NGAL... ELISA Kit
 • Gonadotropin, Equine Chorionic Steroid
 • HSV1 Antibody
 • ELAVL2 Antibody
 • Latrophilin 2 (LPHN2) Recombinant Protein
 • eukaryotic translation initiation factor 4E (EIF4E) Antibody
 • FAIM Antibody
 • Thyroid Stimulating Hormone (TSH) Intact & beta Antibody
 • Tetanus Exotoxin Antibody
 • Homeobox protein Hox-D1 (HOXD1) Recombinant Protein
 • RBM45 Antibody
 downarrow more ...
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PROK1 elisa kit :: Human prokineticin 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703707
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

prokineticin 1 (PROK1), ELISA Kit

 Also Known As   

Human prokineticin 1, PROK1/EG-VEGF ELISA Kit

 Product Synonym Names    Human prokineticin 1 (PROK1/EG-VEGF) ELISA kit; EGVEGF; PK1; PRK1; black mamba toxin-related protein; mambakine; Endocrine gland vascular endothelial growth factor; prokineticin 1
 Product Gene Name   

PROK1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703707 Testing Data    Testing Data PDF
 OMIM    606233
 3D Structure    ModBase 3D Structure for P58294
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PROK1. No significant cross-reactivity or interference between Human PROK1 and analogues was observed.
Section Bar
 Samples    Serum
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Angiogenesis
 Protein Biological Process 3    Angiogenesis
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PROK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PROK1 purchase    MBS703707 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prokineticin 1 (PROK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing PROK1, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS703707 kit is based on PROK1 antibody-PROK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PROK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PROK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar

Related Product Information for PROK1 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EG-VEGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EG-VEGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EG-VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PROK1 elisa kit    PROK1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS703707. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PROK1. It may not necessarily be applicable to this product.
 NCBI GI #    14165282
 NCBI GeneID    84432
 NCBI Accession #    NP_115790.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_032414.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P58294 [Other Products]
 UniProt Secondary Accession #    Q5VWD4; Q8TC69 [Other Products]
 UniProt Related Accession #    P58294 [Other Products]
 Molecular Weight    11,715 Da
Table BarTOPTable Bar
 NCBI Official Full Name    prokineticin-1
 NCBI Official Synonym Full Names    prokineticin 1
 NCBI Official Symbol    PROK1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    prokineticin-1; EG-VEGF; mambakine; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor
 UniProt Protein Name    Prokineticin-1
 UniProt Synonym Protein Names   
Endocrine-gland-derived vascular endothelial growth factor; EG-VEGF; Mambakine
 Protein Family    Prokineticin
 UniProt Gene Name    PROK1 [Similar Products]
 UniProt Synonym Gene Names    UNQ600/PRO1186; EG-VEGF [Similar Products]
 UniProt Entry Name    PROK1_HUMAN
Section Bar
 NCBI Summary for PROK1    The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]
Section Bar
 UniProt Comments for PROK1    Function: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.5 PublicationsManual assertion based on experiment in:Ref.1
Table BarTOPTable Bar
 Research Articles on PROK1    1. Defective placental maturation is associated with an imbalance of expression of basic fibroblast growth factor (FGF) and PK1.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PROK1 elisa kitPathways associated with PROK1 elisa kit
 Reference Product  PubMed Publications
 PROKR1 elisa kit  >26 publications with PROK1 and PROKR1
 PROK2 elisa kit  >23 publications with PROK1 and PROK2
 PROKR2 elisa kit  >16 publications with PROK1 and PROKR2
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with PROK1 elisa kitOrgans/Tissues associated with PROK1 elisa kit
 Disease Name  Pubmed Publications
 Obstetric Labor, Premature Antibodies  >3 publications with PROK1 and Obstetric Labor, Premature
 Premature Birth Antibodies  >3 publications with PROK1 and Premature Birth
 Endometriosis Antibodies  >2 publications with PROK1 and Endometriosis
 Disease Models, Animal Antibodies  >2 publications with PROK1 and Disease Models, Animal
 Hyperplasia Antibodies  >2 publications with PROK1 and Hyperplasia
 Inflammation Antibodies  >2 publications with PROK1 and Inflammation
 Recurrence Antibodies  >1 publications with PROK1 and Recurrence
 Pain Antibodies  >1 publications with PROK1 and Pain
 Nervous System Diseases Antibodies  >1 publications with PROK1 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >15 publications with PROK1 and Placenta
 Uterus Antibodies  >14 publications with PROK1 and Uterus
 Ovary Antibodies  >9 publications with PROK1 and Ovary
 Testis Antibodies  >4 publications with PROK1 and Testis
 Brain Antibodies  >1 publications with PROK1 and Brain
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions