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PROK2 elisa kit :: Human prokineticin 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS940962
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

prokineticin 2 (PROK2), ELISA Kit

★Popular Item★
 Also Known As   

Human Prokineticin-2, PROK2 ELISA Kit

 Product Synonym Names    Human Prokineticin-2 (PROK2) ELISA kit; BV8; KAL4; MIT1; PK2; protein Bv8 homolog; prokineticin 2
 Product Gene Name   

PROK2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS940962 Testing Data    Testing Data PDF
 OMIM    gene 610628
 3D Structure    ModBase 3D Structure for Q9HC23
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PROK2. No significant cross-reactivity or interference between human PROK2 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    6.25 pg/ml-400 pg/ml
 Sensitivity    <1.56 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PROK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PROK2 purchase    MBS940962 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prokineticin 2 (PROK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml-400 pg/ml in biological research samples containing PROK2, with an estimated sensitivity of <1.56 pg/ml. The ELISA analytical biochemical technique of the MBS940962 kit is based on PROK2 antibody-PROK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PROK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PROK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PROK2 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PROK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PROK2 elisa kit    PROK2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS940962. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PROK2. It may not necessarily be applicable to this product.
 NCBI GI #    187167261
 NCBI GeneID    60675
 NCBI Accession #    NP_001119600.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001126128.1 [Other Products]
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 UniProt Primary Accession #    Q9HC23 [Other Products]
 UniProt Secondary Accession #    Q53Z79; Q6ISR0 [Other Products]
 UniProt Related Accession #    Q9HC23 [Other Products]
 Molecular Weight    14,314 Da
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 NCBI Official Full Name    prokineticin-2 isoform a
 NCBI Official Synonym Full Names    prokineticin 2
 NCBI Official Symbol    PROK2 [Similar Products]
 NCBI Official Synonym Symbols   
BV8; HH4; PK2; KAL4; MIT1
[Similar Products]
 NCBI Protein Information    prokineticin-2; protein Bv8 homolog
 UniProt Protein Name    Prokineticin-2
 UniProt Synonym Protein Names   
Protein Bv8 homolog
 Protein Family    Prokineticin
 UniProt Gene Name    PROK2 [Similar Products]
 UniProt Synonym Gene Names    BV8; PK2 [Similar Products]
 UniProt Entry Name    PROK2_HUMAN
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 NCBI Summary for PROK2    This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PROK2    PROK2: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the AVIT (prokineticin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: extracellular region

Molecular Function: G-protein-coupled receptor binding

Biological Process: circadian rhythm; activation of MAPK activity; sensory perception of pain; chemotaxis; cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spermatogenesis; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; negative regulation of apoptosis

Disease: Hypogonadotropic Hypogonadism 4 With Or Without Anosmia
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 Research Articles on PROK2    1. PROK2 signaling in humans is not required for central circadian pacemaker function.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PROK2 elisa kitDiseases associated with PROK2 elisa kit
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Disease Name  Pubmed Publications
 Hypogonadism Antibodies  >44 publications with PROK2 and Hypogonadism
 Kallmann Syndrome Antibodies  >40 publications with PROK2 and Kallmann Syndrome
 Neurologic Manifestations Antibodies  >19 publications with PROK2 and Neurologic Manifestations
 Sensation Disorders Antibodies  >11 publications with PROK2 and Sensation Disorders
 Pain Antibodies  >11 publications with PROK2 and Pain
 Neoplasms Antibodies  >10 publications with PROK2 and Neoplasms
 Inflammation Antibodies  >10 publications with PROK2 and Inflammation
 Hyperalgesia Antibodies  >8 publications with PROK2 and Hyperalgesia
 Heart Diseases Antibodies  >6 publications with PROK2 and Heart Diseases
 Myocardial Infarction Antibodies  >4 publications with PROK2 and Myocardial Infarction
Organs/Tissues associated with PROK2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >41 publications with PROK2 and Brain
 Vascular Antibodies  >39 publications with PROK2 and Vascular
 Testis Antibodies  >8 publications with PROK2 and Testis
 Bone Marrow Antibodies  >6 publications with PROK2 and Bone Marrow
 Placenta Antibodies  >4 publications with PROK2 and Placenta
 Intestine Antibodies  >4 publications with PROK2 and Intestine
 Kidney Antibodies  >3 publications with PROK2 and Kidney
 Spleen Antibodies  >1 publications with PROK2 and Spleen
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