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PDIA3 elisa kit :: Human protein disulfide isomerase family A, member 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700585
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

protein disulfide isomerase family A, member 3 (PDIA3), ELISA Kit

★Popular Item★
 Also Known As   

Human protein disulfide-isomerase A3, PDIA3 ELISA Kit

 Product Synonym Names    Human protein disulfide-isomerase A3; PDIA3 ELISA Kit; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PI-PLC; 58 kDa microsomal protein; endoplasmic reticulum P58; glucose regulated protein; 58kDa; phospholipase C-alpha; protein disulfide isomerase; protein disulfide isomerase family A; member 3
 Product Gene Name   

PDIA3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700585 Testing Data    Testing Data PDF
 OMIM    602046
 3D Structure    ModBase 3D Structure for P30101
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PDIA3. No significant cross-reactivity or interference between human PDIA3 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates.
 Assay Type    Sandwich
 Detection Range    1.25 ng/ml -80 ng/ml.
 Sensitivity    The minimum detectable dose of human PDIA3 is typically less than 0.31 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDIA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDIA3 purchase    MBS700585 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the protein disulfide isomerase family A, member 3 (PDIA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.25 ng/ml -80 ng/ml in biological research samples containing PDIA3, with an estimated sensitivity of The minimum detectable dose of human PDIA3 is typically less than 0.31 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS700585 kit is based on PDIA3 antibody-PDIA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDIA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDIA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PDIA3 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDIA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDIA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PDIA3 elisa kit    PDIA3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700585. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PDIA3. It may not necessarily be applicable to this product.
 NCBI GI #    21361657
 NCBI GeneID    2923
 NCBI Accession #    NP_005304.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_005313.4 [Other Products]
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 UniProt Primary Accession #    P30101 [Other Products]
 UniProt Secondary Accession #    Q13453; Q14255; Q8IYF8; Q9UMU7 [Other Products]
 UniProt Related Accession #    P30101; AAB37397 [Other Products]
 Molecular Weight    56,782 Da
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 NCBI Official Full Name    protein disulfide-isomerase A3
 NCBI Official Synonym Full Names    protein disulfide isomerase family A, member 3
 NCBI Official Symbol    PDIA3 [Similar Products]
 NCBI Official Synonym Symbols   
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
[Similar Products]
 NCBI Protein Information    protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
 UniProt Protein Name    Protein disulfide-isomerase A3
 UniProt Synonym Protein Names   
58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
 Protein Family    Protein disulfide-isomerase
 UniProt Gene Name    PDIA3 [Similar Products]
 UniProt Synonym Gene Names    ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60 [Similar Products]
 UniProt Entry Name    PDIA3_HUMAN
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 NCBI Summary for PDIA3    This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PDIA3    GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: EC 5.3.4.1; Endoplasmic reticulum; Isomerase; Protease; Apoptosis; Chaperone; Phospholipase

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: focal adhesion; cell surface; smooth endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus

Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; protein import into nucleus; cell redox homeostasis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; signal transduction; proteolysis; post-translational protein modification; protein retention in ER
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 Research Articles on PDIA3    1. ERp57 can regulate the expression of the mitochondrial calcium uniporter (MCU) and modulate mitochondrial calcium uptake
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDIA3 elisa kitPathways associated with PDIA3 elisa kit
 Reference Product  PubMed Publications
 STAT3 elisa kit  >12 publications with PDIA3 and STAT3
 HLA-B elisa kit  >7 publications with PDIA3 and HLA-B
 HLA-A elisa kit  >4 publications with PDIA3 and HLA-A
 CALR elisa kit  >3 publications with PDIA3 and CALR
 ERP27 elisa kit  >1 publications with PDIA3 and ERP27
 CANX elisa kit  >1 publications with PDIA3 and CANX
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Calnexin/calreticulin Cycle Pathway antibodies  Calnexin/calreticulin Cycle Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 ER-Phagosome Pathway antibodies  ER-Phagosome Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Diseases associated with PDIA3 elisa kitOrgans/Tissues associated with PDIA3 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >13 publications with PDIA3 and Disease Models, Animal
 Inflammation Antibodies  >10 publications with PDIA3 and Inflammation
 Cardiovascular Diseases Antibodies  >10 publications with PDIA3 and Cardiovascular Diseases
 Liver Diseases Antibodies  >8 publications with PDIA3 and Liver Diseases
 Liver Neoplasms Antibodies  >7 publications with PDIA3 and Liver Neoplasms
 Nervous System Diseases Antibodies  >7 publications with PDIA3 and Nervous System Diseases
 Immune System Diseases Antibodies  >6 publications with PDIA3 and Immune System Diseases
 Brain Diseases Antibodies  >6 publications with PDIA3 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >5 publications with PDIA3 and Carcinoma, Hepatocellular
 Fibrosis Antibodies  >4 publications with PDIA3 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >24 publications with PDIA3 and Blood
 Liver Antibodies  >16 publications with PDIA3 and Liver
 Kidney Antibodies  >12 publications with PDIA3 and Kidney
 Brain Antibodies  >11 publications with PDIA3 and Brain
 Lung Antibodies  >9 publications with PDIA3 and Lung
 Intestine Antibodies  >9 publications with PDIA3 and Intestine
 Bone Antibodies  >7 publications with PDIA3 and Bone
 Heart Antibodies  >5 publications with PDIA3 and Heart
 Muscle Antibodies  >5 publications with PDIA3 and Muscle
 Vascular Antibodies  >5 publications with PDIA3 and Vascular
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