Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PPP2R1A rightarrow Human PPP2R1A  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • FAIM Antibody
 • Taq DNA Polymerase (polA) Recombinant Protein
 • steroidogenic acute regulatory protein (STAR) Antibody
 • Centrosomal protein of 55 kDa (CEP55) ELISA Kit
 • Lactoferrin (LTF) ELISA Kit
 • Monocyte Chemotactic Protein-4 (MCP 4) Recombinant Protein
 • von Willebrand factor A domain containing 1 (VWA1) Antibody
 • SIRP alpha (SIRPA) Recombinant Protein
 • Drop-out Mix Modified w-Succinic Acid and Thiamine w-o Amino... Culture Media
 • Insulin ELISA Kit
 • Fibroblast Growth Factor 7 (FGF7) ELISA Kit
 • PNKP Antibody
 • Ferritin (FE) ELISA Kit
 • ELMO2 ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PPP2R1A elisa kit :: Human protein phosphatase 2 (formerly 2A) , regulatory subunit A, alpha isoform ELISA Kit

Scan QR to view Datasheet Catalog #    MBS926939
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

protein phosphatase 2 (formerly 2A) , regulatory subunit A, alpha isoform (PPP2R1A), ELISA Kit

★Popular Item★
 Also Known As   

Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PPP2R1A ELISA Kit

 Product Synonym Names    Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) ELISA kit; MGC786; PR65A; PP2A; subunit A; PR65-alpha isoform; PP2A; subunit A; R1-alpha isoform; alpha isoform of regulatory subunit A; protein phosphatase 2; medium tumor antigen-associated 61 KDA protein; prote; protein phosphatase 2 (formerly 2A); regulatory subunit A; alpha isoform
 Product Gene Name   

PPP2R1A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS926939 Testing Data    Testing Data PDF
 OMIM    605983
 3D Structure    ModBase 3D Structure for P30153
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of human PPP2R1A. No significant cross-reactivity or interference between human PPP2R1A and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich
 Detection Range    28 pg/ml -1800 pg/ml.
 Sensitivity    7 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PPP2R1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PPP2R1A purchase    MBS926939 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform (PPP2R1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 28 pg/ml -1800 pg/ml in biological research samples containing PPP2R1A, with an estimated sensitivity of 7 pg/ml. The ELISA analytical biochemical technique of the MBS926939 kit is based on PPP2R1A antibody-PPP2R1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPP2R1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPP2R1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for PPP2R1A elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPP2R1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPP2R1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP2R1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP2R1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PPP2R1A elisa kit    PPP2R1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS926939. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PPP2R1A. It may not necessarily be applicable to this product.
 NCBI GI #    21361399
 NCBI GeneID    5518
 NCBI Accession #    NP_055040.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_014225.5 [Other Products]
Section Bar
 UniProt Primary Accession #    P30153 [Other Products]
 UniProt Secondary Accession #    Q13773; Q6ICQ3; Q96DH3 [Other Products]
 UniProt Related Accession #    P30153 [Other Products]
 Molecular Weight    65,309 Da
Table BarTOPTable Bar
 NCBI Official Full Name    serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
 NCBI Official Synonym Full Names    protein phosphatase 2, regulatory subunit A, alpha
 NCBI Official Symbol    PPP2R1A [Similar Products]
 NCBI Official Synonym Symbols   
PR65A; PP2AAALPHA; PP2A-Aalpha
[Similar Products]
 NCBI Protein Information    serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A subunit A isoform R1-alpha; PP2A subunit A isoform PR65-alpha; medium tumor antigen-associated 61 KDA protein; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform
 UniProt Protein Name    Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
 UniProt Synonym Protein Names   
Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha
 UniProt Gene Name    PPP2R1A [Similar Products]
 UniProt Entry Name    2AAA_HUMAN
Section Bar
 NCBI Summary for PPP2R1A    This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
Section Bar
 UniProt Comments for PPP2R1A    PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: microtubule cytoskeleton; protein phosphatase type 2A complex; membrane; mitochondrion; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; protein heterodimerization activity; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity; antigen binding

Biological Process: second-messenger-mediated signaling; regulation of cell adhesion; mitotic nuclear envelope reassembly; fibroblast growth factor receptor signaling pathway; apoptosis; organelle organization and biogenesis; RNA splicing; regulation of Wnt receptor signaling pathway; protein amino acid dephosphorylation; chromosome segregation; response to organic substance; regulation of transcription, DNA-dependent; regulation of growth; regulation of cell differentiation; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; protein complex assembly; gene expression; negative regulation of cell growth; mitotic cell cycle; G2/M transition of mitotic cell cycle; ceramide metabolic process; regulation of DNA replication; inactivation of MAPK activity

Disease: Mental Retardation, Autosomal Dominant 36
Table BarTOPTable Bar
 Research Articles on PPP2R1A    1. The two functional variants in PPP2R1A and PPP2R5E and their combinations are associated with lung cancer risk in the Chinese.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PPP2R1A elisa kitPathways associated with PPP2R1A elisa kit
 Reference Product  PubMed Publications
 PPP2CA elisa kit  >18 publications with PPP2R1A and PPP2CA
 PPP2R2A elisa kit  >2 publications with PPP2R1A and PPP2R2A
 CDK1 elisa kit  >2 publications with PPP2R1A and CDK1
 STRN elisa kit  >1 publications with PPP2R1A and STRN
 HSPD1 elisa kit  >1 publications with PPP2R1A and HSPD1
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Beta-catenin Phosphorylation Cascade Pathway antibodies  Beta-catenin Phosphorylation Cascade Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
Diseases associated with PPP2R1A elisa kitOrgans/Tissues associated with PPP2R1A elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >11 publications with PPP2R1A and Neoplasms
 Adenocarcinoma Antibodies  >4 publications with PPP2R1A and Adenocarcinoma
 Uterine Neoplasms Antibodies  >3 publications with PPP2R1A and Uterine Neoplasms
 Breast Neoplasms Antibodies  >3 publications with PPP2R1A and Breast Neoplasms
 Inflammation Antibodies  >2 publications with PPP2R1A and Inflammation
 Lung Neoplasms Antibodies  >2 publications with PPP2R1A and Lung Neoplasms
 Lung Diseases Antibodies  >1 publications with PPP2R1A and Lung Diseases
 Death Antibodies  >1 publications with PPP2R1A and Death
 Kidney Diseases Antibodies  >1 publications with PPP2R1A and Kidney Diseases
 Kidney Neoplasms Antibodies  >1 publications with PPP2R1A and Kidney Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >3 publications with PPP2R1A and Lung
 Ovary Antibodies  >2 publications with PPP2R1A and Ovary
 Bladder Antibodies  >1 publications with PPP2R1A and Bladder
 Muscle Antibodies  >1 publications with PPP2R1A and Muscle
 Blood Antibodies  >1 publications with PPP2R1A and Blood
 Mammary Gland Antibodies  >1 publications with PPP2R1A and Mammary Gland
 Prostate Antibodies  >1 publications with PPP2R1A and Prostate
 Kidney Antibodies  >1 publications with PPP2R1A and Kidney
 Brain Antibodies  >1 publications with PPP2R1A and Brain
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions