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PKC elisa kit :: Human proteinkinaseC (PKC) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS269666
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

proteinkinaseC (PKC), ELISA Kit

★Popular Item★
 Also Known As   

Human proteinkinaseC (PKC) ELISA Kit

 Product Gene Name   

PKC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant PKC concentration.
 Assay Type    Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PKC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PKC purchase    MBS269666 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the proteinkinaseC (PKC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing PKC, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS269666 kit is based on PKC antibody-PKC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PKC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PKC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant PKC concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PKC elisa kit

   Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PKC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve of PKC elisa kit    PKC elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS269666. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PKC. It may not necessarily be applicable to this product.
 NCBI GI #    6981400
 NCBI GeneID    24681
 NCBI Accession #    NP_036760.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012628.1 [Other Products]
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 UniProt Secondary Accession #    P05697; Q5FWS3 [Other Products]
 UniProt Related Accession #    P63319 [Other Products]
 Molecular Weight    78,358 Da
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 NCBI Official Full Name    protein kinase C gamma type
 NCBI Official Synonym Full Names    protein kinase C, gamma
 NCBI Official Symbol    Prkcg [Similar Products]
 NCBI Official Synonym Symbols   
PKC; PKCI; Prkc; Prkcc; RATPKCI
[Similar Products]
 NCBI Protein Information    protein kinase C gamma type; PKC-gamma; protein kinase C type I (gamma type)
 UniProt Protein Name    Protein kinase C gamma type
 UniProt Gene Name    Prkcg [Similar Products]
 UniProt Synonym Gene Names    Pkcc; Pkcg; Prkcc; PKC-gamma [Similar Products]
 UniProt Entry Name    KPCG_RAT
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 NCBI Summary for PKC    enriched in the neurons of the central nervous system and the spinal cord [RGD, Feb 2006]
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 UniProt Comments for PKC    PKCG: a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. Expressed in the brain and spinal cord where its localization is restricted to neurons. Several neuronal functions, including long term potentiation and depression (LTP&LTD) specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects have been associated with neurodegenerative disorder spinocerebellar ataxia-14. Plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GLUR4 and NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor MGLUR5 and phosphorylates NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43, resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53 and promotes p53-dependent apoptosis in response to DNA damage. Interacts with GRIA4. Interacts with CDCP1. Interacts with TP53INP1 and p53. Expressed in Purkinje cells of the cerebellar cortex.

Protein type: Kinase, protein; Protein kinase, AGC; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Alpha subfamily

Cellular Component: neuron projection; perinuclear region of cytoplasm; cytoplasm; dendrite; plasma membrane; intercellular junction; cytosol; nucleus

Molecular Function: protein binding; protein kinase C activity; zinc ion binding; protein serine/threonine/tyrosine kinase activity; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity

Biological Process: positive regulation of mismatch repair; protein amino acid autophosphorylation; rhythmic process; response to morphine; response to pain; regulation of circadian rhythm; synaptic transmission; learning and/or memory; chemosensory behavior; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; phosphorylation; regulation of response to food
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 Research Articles on PKC    1. Therefore, we propose that PKCgamma positively modulates dopamine release through beta2PIX phosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PKC elisa kitPathways associated with PKC elisa kit
 Reference Product  PubMed Publications
 Prkcd elisa kit  >24 publications with PKC and Prkcd
 Grin2b elisa kit  >1 publications with PKC and Grin2b
 Gja1 elisa kit  >1 publications with PKC and Gja1
 Hras elisa kit  >1 publications with PKC and Hras
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Diseases associated with PKC elisa kitOrgans/Tissues associated with PKC elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >170 publications with PKC and Nervous System Diseases
 Brain Diseases Antibodies  >88 publications with PKC and Brain Diseases
 Pain Antibodies  >82 publications with PKC and Pain
 Ataxia Antibodies  >59 publications with PKC and Ataxia
 Hyperalgesia Antibodies  >43 publications with PKC and Hyperalgesia
 Spinocerebellar Ataxias Antibodies  >38 publications with PKC and Spinocerebellar Ataxias
 Cardiovascular Diseases Antibodies  >27 publications with PKC and Cardiovascular Diseases
 Mental Disorders Antibodies  >24 publications with PKC and Mental Disorders
 Eye Diseases Antibodies  >16 publications with PKC and Eye Diseases
 Brain Ischemia Antibodies  >15 publications with PKC and Brain Ischemia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >240 publications with PKC and Brain
 Ovary Antibodies  >4 publications with PKC and Ovary
 Prostate Antibodies  >1 publications with PKC and Prostate
 Connective Tissue Antibodies  >1 publications with PKC and Connective Tissue
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