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F1+2 elisa kit :: Human prothrombin fragment 1+2, F1+2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701341
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
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10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
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 Product Name   

prothrombin fragment 1+2, F1+2 (F1+2), ELISA Kit

★Popular Item★
 Also Known As   

Human prothrombin fragment 1+2, F1+2 ELISA Kit

 Product Synonym Names    Human prothrombin fragment 1+2; F1+2 ELISA kit; prothrombin fragment 1+2; F1+2
 Product Gene Name   

F1+2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701341 Testing Data    Testing Data PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human F1+2. No significant cross-reactivity or interference between human F1+2 and analogues was observed.
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 Samples    serum, plasma, tissue homogenates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    0.03 nmol/ml-2 nmol/ml.
 Sensitivity    Typically less than 0.007 nmol/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of F1+2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for F1+2 purchase    MBS701341 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prothrombin fragment 1+2, F1+2 (F1+2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.03 nmol/ml-2 nmol/ml in biological research samples containing F1+2, with an estimated sensitivity of Typically less than 0.007 nmol/ml. The ELISA analytical biochemical technique of the MBS701341 kit is based on F1+2 antibody-F1+2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F1+2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F1+2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Intended use of this ELISA Kit is quantitative determination of human prothrombin fragment 1+2 (F1+2) in serum, plasma and tissue homogenates.

Target protein, prothrombin fragment 1+2, is product of in vivo proteolytic cleavage of prothrombin (coagulation Factor II) to thrombin (Factor IIa) by Factor Xa, and its abnormal concentration is indicator of haemostatic disorder; itís used as biomarker in diagnosis of thrombosis and monitoring of anticoagulant therapy. Elevated levels of F1+2 have been reported in patients with venous thromboembolism, acute coronary syndrome, stroke, atrial fibrillation and few other conditions.

Test system is designed as sandwich-type ELISA and concentration of F1+2 is measured using pair of antibodies specific to target protein.

Product contains microwell plate pre-coated with capture antibodies against F1+2 and all reagents required for its quantification, including sample and reagent diluents, and wash solution.

Assay kit provides colorimetric detection of F1+2 using biotinylated detector antibodies against F1+2, avidin-HRP conjugate, and TMB as its substrate solution.

Minimal detectable concentration of ELISA kit is 0.007nmol/mL and its reportable range is 0.03-2.0nmol/mL; concentrated calibratorís preparation for standard curve is provided in lyophilized form and requires dilution for making working levels of calibrators.

Intra- and inter-assay CV values, reported as kitís precision characteristics, are not greater than 8% and 10%, respectively.

Assay doesnít show significant cross-reactivity with analogous proteins.

ELISA kit and its reagents and components require refrigeration and must be stored at 2-8įC until indicated expiration date.
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 Typical Testing Data/Standard Curve (for reference only) of F1+2 elisa kit    F1+2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701341. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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