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PCDHGA3 elisa kit :: Human protocadherin gamma subfamily A, 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS914626
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

protocadherin gamma subfamily A, 3 (PCDHGA3), ELISA Kit

 Also Known As   

Human Protocadherin gamma-A3, PCDHGA3 ELISA Kit

 Product Synonym Names    Human Protocadherin gamma-A3 (PCDHGA3) ELISA kit; PCDH-GAMMA-A3; ; protocadherin gamma subfamily A; 3
 Product Gene Name   

PCDHGA3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS914626 Testing Data    Testing Data PDF
 OMIM    gene 606290
 3D Structure    ModBase 3D Structure for Q9Y5H0
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PCDHGA3. No significant cross-reactivity or interference between human PCDHGA3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml -4000 pg/ml.
 Sensitivity    The minimum detectable dose of human PCDHGA3 is typically less than 15.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PCDHGA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PCDHGA3 purchase    MBS914626 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the protocadherin gamma subfamily A, 3 (PCDHGA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml -4000 pg/ml in biological research samples containing PCDHGA3, with an estimated sensitivity of The minimum detectable dose of human PCDHGA3 is typically less than 15.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS914626 kit is based on PCDHGA3 antibody-PCDHGA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCDHGA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCDHGA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PCDHGA3 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCDHGA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCDHGA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDHGA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHGA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PCDHGA3 elisa kit    PCDHGA3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS914626. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PCDHGA3. It may not necessarily be applicable to this product.
 NCBI GI #    14589880
 NCBI GeneID    56112
 NCBI Accession #    NP_061739.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_018916.3 [Other Products]
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 UniProt Primary Accession #    Q9Y5H0 [Other Products]
 UniProt Secondary Accession #    Q9Y5D4 [Other Products]
 UniProt Related Accession #    Q9Y5H0 [Other Products]
 Molecular Weight    100,971 Da
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 NCBI Official Full Name    protocadherin gamma-A3 isoform 1
 NCBI Official Synonym Full Names    protocadherin gamma subfamily A, 3
 NCBI Official Symbol    PCDHGA3 [Similar Products]
 NCBI Official Synonym Symbols   
PCDH-GAMMA-A3
[Similar Products]
 NCBI Protein Information    protocadherin gamma-A3
 UniProt Protein Name    Protocadherin gamma-A3
 Protein Family    Protocadherin
 UniProt Gene Name    PCDHGA3 [Similar Products]
 UniProt Synonym Gene Names    PCDH-gamma-A3 [Similar Products]
 UniProt Entry Name    PCDG3_HUMAN
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 NCBI Summary for PCDHGA3    This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PCDHGA3    PCDHGA3: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: homophilic cell adhesion
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 Research Articles on PCDHGA3    1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with PCDHGA3 elisa kitOrgans/Tissues associated with PCDHGA3 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with PCDHGA3 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Pineal Gland Antibodies  >1 publications with PCDHGA3 and Pineal Gland
 Brain Antibodies  >1 publications with PCDHGA3 and Brain
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