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ROMO1 elisa kit :: Human reactive oxygen species modulator 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS906890
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

reactive oxygen species modulator 1 (ROMO1), ELISA Kit

 Also Known As   

Human Reactive oxygen species modulator 1, ROMO1 ELISA Kit

 Product Synonym Names    Human Reactive oxygen species modulator 1 (ROMO1) ELISA kit; RP11-353C18.54; C20orf52; MGC111180; MTGMP; bA353C18.2; OTTHUMP00000062052; OTTHUMP00000196684; mitochondrial targeting GXXXG protein; reactive oxygen species modulator 1
 Product Gene Name   

ROMO1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS906890 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P60602
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human ROMO1. No significant cross-reactivity or interference between human ROMO1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Sandwich
 Detection Range    18.8 pg/ml -1200 pg/ml
 Sensitivity    4.7 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ROMO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ROMO1 purchase    MBS906890 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the reactive oxygen species modulator 1 (ROMO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.8 pg/ml -1200 pg/ml in biological research samples containing ROMO1, with an estimated sensitivity of 4.7 pg/ml. The ELISA analytical biochemical technique of the MBS906890 kit is based on ROMO1 antibody-ROMO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROMO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROMO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ROMO1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ROMO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROMO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROMO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROMO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ROMO1 elisa kit    ROMO1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS906890. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ROMO1. It may not necessarily be applicable to this product.
 NCBI GI #    18152785
 NCBI GeneID    140823
 NCBI Accession #    NP_542786.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_080748.2 [Other Products]
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 UniProt Primary Accession #    P60602 [Other Products]
 UniProt Secondary Accession #    Q3MHD5; Q5QP16; Q9CQ98; Q9H1N2; A7M872; E1P5R9; E9KL28 [Other Products]
 UniProt Related Accession #    P60602 [Other Products]
 Molecular Weight    8,183 Da
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 NCBI Official Full Name    reactive oxygen species modulator 1
 NCBI Official Synonym Full Names    reactive oxygen species modulator 1
 NCBI Official Symbol    ROMO1 [Similar Products]
 NCBI Official Synonym Symbols   
MTGM; MTGMP; C20orf52; bA353C18.2
[Similar Products]
 NCBI Protein Information    reactive oxygen species modulator 1; glyrichin; ROS modulator 1; protein MGR2 homolog; epididymis tissue protein Li 175; mitochondrial targeting GXXXG protein; mitochondrial targeting GxxxG motif protein
 UniProt Protein Name    Reactive oxygen species modulator 1
 UniProt Synonym Protein Names   
Epididymis tissue protein Li 175; Glyrichin; Mitochondrial targeting GxxxG motif protein; MTGM; Protein MGR2 homolog
 Protein Family    Reactive oxygen species modulator
 UniProt Gene Name    ROMO1 [Similar Products]
 UniProt Synonym Gene Names    C20orf52; ROS modulator 1; MTGM [Similar Products]
 UniProt Entry Name    ROMO1_HUMAN
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 NCBI Summary for ROMO1    The protein encoded by this gene is a mitochondrial membrane protein that is responsible for increasing the level of reactive oxygen species (ROS) in cells. The protein also has antimicrobial activity against a variety of bacteria by inducing bacterial membrane breakage. [provided by RefSeq, Nov 2014]
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 UniProt Comments for ROMO1    ROMO1: Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Belongs to the MGR2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Biological Process: positive regulation of cell proliferation; defense response to bacterium; replicative cell aging
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 Research Articles on ROMO1    1. This study is the first to demonstrate that strict modulation of DKK1 expression in different cell types partially maintains cell survival via tight regulation of the ROS-producing ROMO1 and radiation resistance.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with ROMO1 elisa kitOrgans/Tissues associated with ROMO1 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with ROMO1 and Neoplasms
 Necrosis Antibodies  >2 publications with ROMO1 and Necrosis
 Translocation, Genetic Antibodies  >1 publications with ROMO1 and Translocation, Genetic
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with ROMO1 and Lung
 Brain Antibodies  >2 publications with ROMO1 and Brain
 Blood Antibodies  >1 publications with ROMO1 and Blood
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