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REN elisa kit :: Human renin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703134
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

renin, ELISA Kit

★Popular Item★
 Also Known As   

Human renin, REN ELISA Kit

 Product Synonym Names    Human renin (REN) ELISA Kit; FLJ10761; HNFJ2; angiotensin-forming enzyme; angiotensinogenase; renin precursor; renal; renin
 Product Gene Name   

REN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703134 Testing Data    Testing Data PDF
 OMIM    613092
 3D Structure    ModBase 3D Structure for P00797
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human REN. No significant cross-reactivity or interference between Human REN and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Detection Range    15.6 mIU/ml-1000 mIU/ml
 Sensitivity    3.9 mIU/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of REN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for REN purchase    MBS703134 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the renin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 mIU/ml-1000 mIU/ml in biological research samples containing REN, with an estimated sensitivity of 3.9 mIU/ml. The ELISA analytical biochemical technique of the MBS703134 kit is based on REN antibody-REN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for REN elisa kit

   Introduction: Renin, also known as Angiotensinogenase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume, and arterial vasoconstriction. Thus it regulates the body's mean arterial blood pressure. The primary structure of renin precursor consists of 406 amino acids with a pre- and a pro- segment carrying 20 and 46 amino acids respectively. Mature renin contains 340 amino acids and has a mass of 37 kD. The peptide hormone is secreted by the kidney from specialized cells called granular cells of the juxtaglomerular apparatus in response to a decrease in arterial blood pressure as detected by baroreceptors. This is the most causal link between blood pressure and renin secretion (the other two methods operate via longer pathways), and to a decrease in sodium chloride levels in the ultra-filtrate of the nephron.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to renin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for renin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain renin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of renin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of REN elisa kit    REN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703134. Click to request current manual
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NCBI/Uniprot data below describe general gene information for REN. It may not necessarily be applicable to this product.
 NCBI GI #    4506475
 NCBI GeneID    5972
 NCBI Accession #    NP_000528.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000537.3 [Other Products]
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 UniProt Primary Accession #    P00797 [Other Products]
 UniProt Secondary Accession #    Q6FI38; Q6T5C2 [Other Products]
 UniProt Related Accession #    P00797 [Other Products]
 Molecular Weight    45,057 Da
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 NCBI Official Full Name    renin preproprotein
 NCBI Official Synonym Full Names    renin
 NCBI Official Symbol    REN [Similar Products]
 NCBI Official Synonym Symbols   
HNFJ2
[Similar Products]
 NCBI Protein Information    renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme
 UniProt Protein Name    Renin
 UniProt Synonym Protein Names   
Angiotensinogenase
 Protein Family    Renin
 UniProt Gene Name    REN [Similar Products]
 UniProt Entry Name    RENI_HUMAN
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 NCBI Summary for REN    Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for REN    Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activity: Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulation: Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.
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 Research Articles on REN    1. Data suggest that the renin-angiotensin system plays a critical role in placentation and nephrogenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with REN elisa kitPathways associated with REN elisa kit
 Reference Product  PubMed Publications
 ACE elisa kit  >143 publications with REN and ACE
 PTGS2 elisa kit  >72 publications with REN and PTGS2
 AGT elisa kit  >56 publications with REN and AGT
 INS elisa kit  >31 publications with REN and INS
 NOS3 elisa kit  >23 publications with REN and NOS3
 ATP6AP2 elisa kit  >4 publications with REN and ATP6AP2
 RENBP elisa kit  >2 publications with REN and RENBP
 DIF elisa kit  >1 publications with REN and DIF
 EDN1 elisa kit  >1 publications with REN and EDN1
 KNG1 elisa kit  >1 publications with REN and KNG1
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
Diseases associated with REN elisa kitOrgans/Tissues associated with REN elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >4271 publications with REN and Kidney Diseases
 Renal Insufficiency Antibodies  >2899 publications with REN and Renal Insufficiency
 Hypertension Antibodies  >1202 publications with REN and Hypertension
 Heart Diseases Antibodies  >1084 publications with REN and Heart Diseases
 Necrosis Antibodies  >876 publications with REN and Necrosis
 Acute Kidney Injury Antibodies  >872 publications with REN and Acute Kidney Injury
 Hypertension, Renal Antibodies  >611 publications with REN and Hypertension, Renal
 Ischemia Antibodies  >586 publications with REN and Ischemia
 Proteinuria Antibodies  >496 publications with REN and Proteinuria
 Fibrosis Antibodies  >494 publications with REN and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >678 publications with REN and Kidney
 Lung Antibodies  >611 publications with REN and Lung
 Skin Antibodies  >322 publications with REN and Skin
 Intestine Antibodies  >213 publications with REN and Intestine
 Embryonic Tissue Antibodies  >167 publications with REN and Embryonic Tissue
 Testis Antibodies  >96 publications with REN and Testis
 Pancreas Antibodies  >83 publications with REN and Pancreas
 Ovary Antibodies  >81 publications with REN and Ovary
 Bladder Antibodies  >70 publications with REN and Bladder
 Placenta Antibodies  >55 publications with REN and Placenta
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