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RHO elisa kit :: Human rhodopsin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS910995
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

rhodopsin, ELISA Kit

★Popular Item★
 Also Known As   

Human Rhodopsin, RHO ELISA Kit

 Product Synonym Names    Human Rhodopsin (RHO) ELISA kit; CSNBAD1; MGC138309; MGC138311; OPN2; RP4; opsin 2; rod pigment; retinitis pigmentosa 4; autosomal dominant; rhodopsin (opsin 2; rod pigment) (retinitis pigmentosa 4; autosomal dominant) ; rhodopsin
 Product Gene Name   

RHO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS910995 Testing Data    Testing Data PDF
 OMIM    613731
 3D Structure    ModBase 3D Structure for P08100
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human RHO. No significant cross-reactivity or interference between human RHO and analogues was observed.
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 Samples    serum, plasma, tissue homogenates and cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    12.5 pg/ml -800 pg/ml
 Sensitivity    <3.12 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RHO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RHO purchase    MBS910995 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the rhodopsin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 pg/ml -800 pg/ml in biological research samples containing RHO, with an estimated sensitivity of <3.12 pg/ml. The ELISA analytical biochemical technique of the MBS910995 kit is based on RHO antibody-RHO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RHO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RHO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates and cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RHO elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RHO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RHO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHO bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of RHO elisa kit    RHO elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS910995. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RHO. It may not necessarily be applicable to this product.
 NCBI GI #    4506527
 NCBI GeneID    6010
 NCBI Accession #    NP_000530.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000539.3 [Other Products]
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 UniProt Primary Accession #    P08100 [Other Products]
 UniProt Secondary Accession #    Q16414; Q2M249 [Other Products]
 UniProt Related Accession #    P08100 [Other Products]
 Molecular Weight    38,893 Da
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 NCBI Official Full Name    rhodopsin
 NCBI Official Synonym Full Names    rhodopsin
 NCBI Official Symbol    RHO [Similar Products]
 NCBI Official Synonym Symbols   
RP4; OPN2; CSNBAD1
[Similar Products]
 NCBI Protein Information    rhodopsin; opsin-2; opsin 2, rod pigment
 UniProt Protein Name    Rhodopsin
 UniProt Synonym Protein Names   
Opsin-2
 Protein Family    Rhodopsin
 UniProt Gene Name    RHO [Similar Products]
 UniProt Synonym Gene Names    OPN2 [Similar Products]
 UniProt Entry Name    OPSD_HUMAN
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 NCBI Summary for RHO    Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RHO    Rhodopsin: a G-protein coupled receptor. The light-absorbing visual pigment in Rod photoreceptor cells. Mediates vision in dim light. Consists of the apoprotein, opsin, covalently linked to cis-retinal. Defects in RHO are a cause of autosomal retinitis pigmentosa and congenital stationary night blindness.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q21-q24

Cellular Component: Golgi membrane; Golgi apparatus; photoreceptor outer segment; photoreceptor inner segment; integral to plasma membrane; plasma membrane; rough endoplasmic reticulum membrane; intercellular junction

Molecular Function: G-protein coupled receptor activity; protein binding; spectrin binding; metal ion binding; photoreceptor activity; retinal binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; G-protein coupled receptor protein signaling pathway; red, far-red light phototransduction; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; organelle organization and biogenesis; retinoid metabolic process; protein amino acid phosphorylation; protein-chromophore linkage

Disease: Fundus Albipunctatus; Night Blindness, Congenital Stationary, Autosomal Dominant 1; Retinitis Pigmentosa 4
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 Research Articles on RHO    1. These studies indicate that activation of either IRE1, ATF6, or PERK prevents mutant rhodopsin from accumulating in the cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RHO elisa kitPathways associated with RHO elisa kit
 Reference Product  PubMed Publications
 NRL elisa kit  >30 publications with RHO and NRL
 GRK1 elisa kit  >15 publications with RHO and GRK1
 GNAT1 elisa kit  >12 publications with RHO and GNAT1
 ADRBK1 elisa kit  >11 publications with RHO and ADRBK1
 SAG elisa kit  >6 publications with RHO and SAG
 GNAT2 elisa kit  >2 publications with RHO and GNAT2
 GNGT1 elisa kit  >1 publications with RHO and GNGT1
 GRK7 elisa kit  >1 publications with RHO and GRK7
 GRK5 elisa kit  >1 publications with RHO and GRK5
 Products by Pathway  Pathway Diagram
 Activation Of The Phototransduction Cascade Pathway antibodies  Activation Of The Phototransduction Cascade Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway antibodies  Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
Diseases associated with RHO elisa kitOrgans/Tissues associated with RHO elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1239 publications with RHO and Inflammation
 Lung Diseases Antibodies  >1041 publications with RHO and Lung Diseases
 Heart Diseases Antibodies  >988 publications with RHO and Heart Diseases
 Endocrine System Diseases Antibodies  >794 publications with RHO and Endocrine System Diseases
 Necrosis Antibodies  >616 publications with RHO and Necrosis
 Neoplasm Metastasis Antibodies  >614 publications with RHO and Neoplasm Metastasis
 Liver Diseases Antibodies  >552 publications with RHO and Liver Diseases
 Fibrosis Antibodies  >535 publications with RHO and Fibrosis
 Kidney Diseases Antibodies  >480 publications with RHO and Kidney Diseases
 Hypertrophy Antibodies  >324 publications with RHO and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2579 publications with RHO and Muscle
 Brain Antibodies  >1919 publications with RHO and Brain
 Eye Antibodies  >891 publications with RHO and Eye
 Uncharacterized Tissue Antibodies  >6 publications with RHO and Uncharacterized Tissue
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