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RRBP1 elisa kit :: Human ribosome binding protein 1 homolog 180kDa (dog) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9329000 RRBP1 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
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 Product Name   

ribosome binding protein 1 homolog 180kDa (dog) (RRBP1), ELISA Kit

★Popular Item★
 Also Known As   

Human Ribosome-binding protein 1, RRBP1 ELISA Kit

 Product Synonym Names    Human Ribosome-binding protein 1 (RRBP1) ELISA kit; DKFZp586A1420; ES/130; ES130; FLJ36146; MGC157720; MGC157721; hES; ribosome binding protein 1; ribosome binding protein 1 homolog 180kDa (dog)
 Product Gene Name   

RRBP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601418
 3D Structure    ModBase 3D Structure for Q9P2E9
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between Human RRBP1 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    31.2 ng/ml - 1000 ng/ml.
 Sensitivity    5.0 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RRBP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RRBP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RRBP1 and RRBP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RRBP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RRBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RRBP1 purchase    MBS9329000 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ribosome binding protein 1 homolog 180kDa (dog) (RRBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 ng/ml - 1000 ng/ml in biological research samples containing RRBP1, with an estimated sensitivity of 5.0 ng/ml. The ELISA analytical biochemical technique of the MBS9329000 kit is based on RRBP1 antibody-RRBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RRBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RRBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RRBP1 elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RRBP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RRBP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RRBP1 and RRBP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RRBP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS9329000. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RRBP1. It may not necessarily be applicable to this product.
 NCBI GI #    110611218
 NCBI GeneID    6238
 NCBI Accession #    NP_001036041.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001042576.1 [Other Products]
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 UniProt Primary Accession #    Q9P2E9 [Other Products]
 UniProt Secondary Accession #    O75300; O75301; Q5W165; Q96SB2; Q9BWP1; Q9H476; A2A2S6; A6NCN6 [Other Products]
 UniProt Related Accession #    Q9P2E9 [Other Products]
 Molecular Weight    152,472 Da
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 NCBI Official Full Name    ribosome-binding protein 1
 NCBI Official Synonym Full Names    ribosome binding protein 1
 NCBI Official Symbol    RRBP1 [Similar Products]
 NCBI Official Synonym Symbols   
RRp; hES; ES130; ES/130
[Similar Products]
 NCBI Protein Information    ribosome-binding protein 1; ES/130-related protein; ribosome receptor protein; 180 kDa ribosome receptor homolog; ribosome binding protein 1 homolog 180kDa (dog)
 UniProt Protein Name    Ribosome-binding protein 1
 UniProt Synonym Protein Names   
180 kDa ribosome receptor homolog; RRp; ES/130-related protein; Ribosome receptor protein
 Protein Family    Ribosome-binding protein
 UniProt Gene Name    RRBP1 [Similar Products]
 UniProt Synonym Gene Names    KIAA1398; RRp [Similar Products]
 UniProt Entry Name    RRBP1_HUMAN
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 NCBI Summary for RRBP1    This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]
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 UniProt Comments for RRBP1    RRBP1: a single-pass type III membrane protein of the rough endoplasmic reticulum membrane. Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Associated with the unfolded protein response (UPR) and has anti-apoptotic activity. Knockdown of RRBP1 induces ER stress, and is associated with a significant reduction in the expression of glucose-regulated protein 78 (GRP78). GRP78 regulates the UPR, is widely upregulated in cancer, and plays an important role in chemotherapy resistance in some cancers. Upregulated in many cancers: overexpressed in a high proportion of breast carcinoma cases tested, and is highly expressed in lung cancer tissue compared with adjacent normal tissues. Because RRBP1 appears to play a role in tumor cell survival, it may be a candidate for new targeted therapeutics. Three human isoforms are produced by alternative splicing.

Protein type: Translation; Membrane protein, integral; Ribosomal

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; ribosome

Molecular Function: receptor activity

Biological Process: osteoblast differentiation; protein transport; translation
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 Research Articles on RRBP1    1. Data indicate that p180 is required for the efficient targeting of placental alkaline phosphatase (ALPP) mRNA to the endoplasmic reticulum (ER).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with RRBP1 elisa kitDiseases associated with RRBP1 elisa kit
 Products by Pathway  Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1 publications with RRBP1 and Carcinoma
 Muscular Diseases Antibodies  >1 publications with RRBP1 and Muscular Diseases
 Nervous System Diseases Antibodies  >1 publications with RRBP1 and Nervous System Diseases
 Neoplasms Antibodies  >1 publications with RRBP1 and Neoplasms
Organs/Tissues associated with RRBP1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with RRBP1 and Muscle
 Intestine Antibodies  >1 publications with RRBP1 and Intestine
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