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SPARC elisa kit :: Human secreted protein, acidic, cysteine-rich (osteonectin) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS921942
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

secreted protein, acidic, cysteine-rich (osteonectin) (SPARC), ELISA Kit

★Popular Item★
 Also Known As   

Human SPARC, SPARC ELISA Kit

 Product Synonym Names    Human SPARC (SPARC) ELISA kit; ON; cysteine-rich protein; osteonectin; secreted protein; acidic; cysteine-rich; secreted protein; acidic; cysteine-rich (osteonectin)
 Product Gene Name   

SPARC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS921942 Testing Data    Testing Data PDF
 OMIM    182120
 3D Structure    ModBase 3D Structure for P09486
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human SPARC. No significant cross-reactivity or interference between human SPARC and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, urine
 Assay Type    Sandwich
 Detection Range    62.5 ng/ml - 4000 ng/ml.
 Sensitivity    The minimum detectable dose of human SPARC is typically less than 15.6 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SPARC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SPARC purchase    MBS921942 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the secreted protein, acidic, cysteine-rich (osteonectin) (SPARC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 ng/ml - 4000 ng/ml in biological research samples containing SPARC, with an estimated sensitivity of The minimum detectable dose of human SPARC is typically less than 15.6 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS921942 kit is based on SPARC antibody-SPARC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPARC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPARC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SPARC elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPARC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPARC bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SPARC elisa kit    SPARC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS921942. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SPARC. It may not necessarily be applicable to this product.
 NCBI GI #    4507171
 NCBI GeneID    6678
 NCBI Accession #    NP_003109.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003118.3 [Other Products]
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 UniProt Primary Accession #    P09486 [Other Products]
 UniProt Secondary Accession #    Q6IBK4; D3DQH9 [Other Products]
 UniProt Related Accession #    P09486 [Other Products]
 Molecular Weight    34,632 Da
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 NCBI Official Full Name    SPARC
 NCBI Official Synonym Full Names    secreted protein, acidic, cysteine-rich (osteonectin)
 NCBI Official Symbol    SPARC [Similar Products]
 NCBI Official Synonym Symbols   
ON
[Similar Products]
 NCBI Protein Information    SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
 UniProt Protein Name    SPARC
 UniProt Synonym Protein Names   
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
 Protein Family    SPARC
 UniProt Gene Name    SPARC [Similar Products]
 UniProt Synonym Gene Names    ON; BM-40; ON [Similar Products]
 UniProt Entry Name    SPRC_HUMAN
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 NCBI Summary for SPARC    This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]
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 UniProt Comments for SPARC    SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; extracellular region; plasma membrane; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: response to peptide hormone stimulus; platelet activation; response to gravity; receptor-mediated endocytosis; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; response to ethanol; platelet degranulation; response to cadmium ion; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii
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 Research Articles on SPARC    1. We observed a positive correlation of osteonectin expression in connective-type stromal tissue surrounding the epithelial tumor cells of craniopharyngioma with the extent of central nervous system infiltration and recurrence rate
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SPARC elisa kitPathways associated with SPARC elisa kit
 Reference Product  PubMed Publications
 TGFB1 elisa kit  >57 publications with SPARC and TGFB1
 THBS1 elisa kit  >48 publications with SPARC and THBS1
 COL1A2 elisa kit  >28 publications with SPARC and COL1A2
 COL1A1 elisa kit  >22 publications with SPARC and COL1A1
 COL3A1 elisa kit  >17 publications with SPARC and COL3A1
 FN1 elisa kit  >9 publications with SPARC and FN1
 CTSK elisa kit  >9 publications with SPARC and CTSK
 COL2A1 elisa kit  >5 publications with SPARC and COL2A1
 VEGFA elisa kit  >4 publications with SPARC and VEGFA
 PLAT elisa kit  >3 publications with SPARC and PLAT
 Products by Pathway  Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with SPARC elisa kitOrgans/Tissues associated with SPARC elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >492 publications with SPARC and Neoplasms
 Carcinoma Antibodies  >163 publications with SPARC and Carcinoma
 Cardiovascular Diseases Antibodies  >88 publications with SPARC and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >83 publications with SPARC and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >80 publications with SPARC and Neoplasm Metastasis
 Inflammation Antibodies  >77 publications with SPARC and Inflammation
 Fibrosis Antibodies  >73 publications with SPARC and Fibrosis
 Gastrointestinal Diseases Antibodies  >62 publications with SPARC and Gastrointestinal Diseases
 Brain Diseases Antibodies  >59 publications with SPARC and Brain Diseases
 Breast Neoplasms Antibodies  >54 publications with SPARC and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >947 publications with SPARC and Bone
 Connective Tissue Antibodies  >408 publications with SPARC and Connective Tissue
 Blood Antibodies  >289 publications with SPARC and Blood
 Vascular Antibodies  >259 publications with SPARC and Vascular
 Bone Marrow Antibodies  >174 publications with SPARC and Bone Marrow
 Embryonic Tissue Antibodies  >155 publications with SPARC and Embryonic Tissue
 Muscle Antibodies  >148 publications with SPARC and Muscle
 Mouth Antibodies  >140 publications with SPARC and Mouth
 Brain Antibodies  >121 publications with SPARC and Brain
 Skin Antibodies  >104 publications with SPARC and Skin
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