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SIRT4 elisa kit :: Human sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS706224
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) (SIRT4), ELISA Kit

 Also Known As   

Human NAD-dependent ADP-ribosyltransferase sirtuin-4, SIRT4/SIR2L4 ELISA Kit

 Product Synonym Names    Human NAD-dependent ADP-ribosyltransferase sirtuin-4 (SIRT4/SIR2L4) ELISA kit; MGC130046; MGC130047; MGC57437; SIR2L4; sir2-like 4; sirtuin 4; sirtuin type 4; sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
 Product Gene Name   

SIRT4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS706224 Testing Data    Testing Data PDF
 OMIM    604482
 3D Structure    ModBase 3D Structure for Q9Y6E7
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human SIRT4. No significant cross-reactivity or interference between Human SIRT4 and analogues was observed.
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 Assay Type    Sandwich
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SIRT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SIRT4 purchase    MBS706224 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) (SIRT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SIRT4. The ELISA analytical biochemical technique of the MBS706224 kit is based on SIRT4 antibody-SIRT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIRT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIRT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SIRT4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIRT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SIRT4 elisa kit    SIRT4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS706224. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SIRT4. It may not necessarily be applicable to this product.
 NCBI GI #    6912662
 NCBI GeneID    23409
 NCBI Accession #    NP_036372.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012240.2 [Other Products]
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 UniProt Primary Accession #    Q9Y6E7 [Other Products]
 UniProt Secondary Accession #    O43346; Q32M33 [Other Products]
 UniProt Related Accession #    Q9Y6E7 [Other Products]
 Molecular Weight    35,188 Da
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 NCBI Official Full Name    NAD-dependent protein deacetylase sirtuin-4
 NCBI Official Synonym Full Names    sirtuin 4
 NCBI Official Symbol    SIRT4 [Similar Products]
 NCBI Official Synonym Symbols   
SIR2L4
[Similar Products]
 NCBI Protein Information    NAD-dependent protein deacetylase sirtuin-4; sir2-like 4; sirtuin type 4; SIR2-like protein 4; regulatory protein SIR2 homolog 4; NAD-dependent ADP-ribosyltransferase sirtuin-4
 UniProt Protein Name    NAD-dependent protein deacetylase sirtuin-4
 UniProt Synonym Protein Names   
NAD-dependent ADP-ribosyltransferase sirtuin-4 (EC:2.4.2.-); Regulatory protein SIR2 homolog 4; SIR2-like protein 4
 Protein Family    NAD-dependent protein deacylase
 UniProt Gene Name    SIRT4 [Similar Products]
 UniProt Synonym Gene Names    SIR2L4 [Similar Products]
 UniProt Entry Name    SIR4_HUMAN
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 NCBI Summary for SIRT4    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SIRT4    Function: Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.4 PublicationsManual assertion based on experiment in:Ref.4

Catalytic activity: NAD+ + a protein = nicotinamide + an N-(ADP-D-ribosyl)-protein.UniRule annotationHAMAP-Rule:MF_03161

Cofactor: Binds 1 zinc ion per subunit UniRule annotationHAMAP-Rule:MF_03161
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 Research Articles on SIRT4    1. regulates ATP levels via ANT2 and a feedback loop involving AMPK
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SIRT4 elisa kitPathways associated with SIRT4 elisa kit
 Reference Product  PubMed Publications
 SIRT3 elisa kit  >42 publications with SIRT4 and SIRT3
 GLUD1 elisa kit  >3 publications with SIRT4 and GLUD1
 HDAC9 elisa kit  >2 publications with SIRT4 and HDAC9
 HDAC11 elisa kit  >1 publications with SIRT4 and HDAC11
 Products by Pathway  Pathway Diagram
 Signaling Events Mediated By HDAC Class I Pathway antibodies  Signaling Events Mediated By HDAC Class I Pathway Diagram
Diseases associated with SIRT4 elisa kitOrgans/Tissues associated with SIRT4 elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >6 publications with SIRT4 and Fatty Liver
 Hypoglycemia Antibodies  >3 publications with SIRT4 and Hypoglycemia
 Necrosis Antibodies  >2 publications with SIRT4 and Necrosis
 Inflammation Antibodies  >2 publications with SIRT4 and Inflammation
 Brain Diseases Antibodies  >1 publications with SIRT4 and Brain Diseases
 Abnormalities, Drug-Induced Antibodies  >1 publications with SIRT4 and Abnormalities, Drug-Induced
 Kidney Diseases Antibodies  >1 publications with SIRT4 and Kidney Diseases
 Lung Diseases Antibodies  >1 publications with SIRT4 and Lung Diseases
 Fibrosis Antibodies  >1 publications with SIRT4 and Fibrosis
 Proteinuria Antibodies  >1 publications with SIRT4 and Proteinuria
 Organ/Tissue Name  Pubmed Publications
 Fat Body Antibodies  >1 publications with SIRT4 and Fat Body
 Whole Body Antibodies  >1 publications with SIRT4 and Whole Body
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