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SLIT2 elisa kit :: Human slit homolog 2 (Drosophila) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703756
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

slit homolog 2 (Drosophila) (SLIT2), ELISA Kit

★Popular Item★
 Also Known As   

Human Slit2 ELISA Kit

 Product Synonym Names    Human Slit2 ELISA Kit; FLJ14420; SLIL3; Slit-2; slit homolog 2; slit homolog 2 (Drosophila)
 Product Gene Name   

SLIT2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703756 Testing Data    Testing Data PDF
 OMIM    603746
 3D Structure    ModBase 3D Structure for O94813
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human Slit2. No significant cross-reactivity or interference between human Slit2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.78 ng/ml -50 ng/ml
 Sensitivity    0.195 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SLIT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SLIT2 purchase    MBS703756 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the slit homolog 2 (Drosophila) (SLIT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78 ng/ml -50 ng/ml in biological research samples containing SLIT2, with an estimated sensitivity of 0.195 ng/ml. The ELISA analytical biochemical technique of the MBS703756 kit is based on SLIT2 antibody-SLIT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLIT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLIT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SLIT2 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Slit2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Slit2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Slit2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Slit2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SLIT2 elisa kit    SLIT2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703756. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SLIT2. It may not necessarily be applicable to this product.
 NCBI GI #    574281850
 NCBI GeneID    9353
 NCBI Accession #    NP_001276064.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001289135.1 [Other Products]
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 UniProt Primary Accession #    O94813 [Other Products]
 UniProt Secondary Accession #    O95710; Q17RU3; Q9Y5Q7; B7ZLR5 [Other Products]
 UniProt Related Accession #    O94813 [Other Products]
 Molecular Weight    169,870 Da
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 NCBI Official Full Name    slit homolog 2 protein isoform 2
 NCBI Official Synonym Full Names    slit homolog 2 (Drosophila)
 NCBI Official Symbol    SLIT2 [Similar Products]
 NCBI Official Synonym Symbols   
SLIL3; Slit-2
[Similar Products]
 NCBI Protein Information    slit homolog 2 protein
 UniProt Protein Name    Slit homolog 2 protein
 Protein Family    Slit homolog 2 protein
 UniProt Gene Name    SLIT2 [Similar Products]
 UniProt Synonym Gene Names    SLIL3; Slit-2 [Similar Products]
 UniProt Entry Name    SLIT2_HUMAN
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 NCBI Summary for SLIT2    This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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 UniProt Comments for SLIT2    Function: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.6 PublicationsManual assertion based on experiment in:Ref.3
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 Research Articles on SLIT2    1. Biological activity of Slit2 and its binding to Robo1 have no affect on the dimer formation of Robo1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SLIT2 elisa kitPathways associated with SLIT2 elisa kit
 Reference Product  PubMed Publications
 ROBO1 elisa kit  >108 publications with SLIT2 and ROBO1
 ROBO2 elisa kit  >66 publications with SLIT2 and ROBO2
 DCC elisa kit  >9 publications with SLIT2 and DCC
 Products by Pathway  Pathway Diagram
 Activation Of Rac Pathway antibodies  Activation Of Rac Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Glypican 1 Network Pathway antibodies  Glypican 1 Network Pathway Diagram
 Inactivation Of Cdc42 And Rac Pathway antibodies  Inactivation Of Cdc42 And Rac Pathway Diagram
 Netrin-1 Signaling Pathway antibodies  Netrin-1 Signaling Pathway Diagram
 Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway antibodies  Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway Diagram
 Role Of Abl In Robo-Slit Signaling Pathway antibodies  Role Of Abl In Robo-Slit Signaling Pathway Diagram
Diseases associated with SLIT2 elisa kitOrgans/Tissues associated with SLIT2 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >23 publications with SLIT2 and Breast Neoplasms
 Nervous System Diseases Antibodies  >20 publications with SLIT2 and Nervous System Diseases
 Lung Neoplasms Antibodies  >16 publications with SLIT2 and Lung Neoplasms
 Skin Diseases Antibodies  >15 publications with SLIT2 and Skin Diseases
 Neoplasm Metastasis Antibodies  >13 publications with SLIT2 and Neoplasm Metastasis
 Brain Diseases Antibodies  >11 publications with SLIT2 and Brain Diseases
 Inflammation Antibodies  >10 publications with SLIT2 and Inflammation
 Liver Diseases Antibodies  >6 publications with SLIT2 and Liver Diseases
 Disease Models, Animal Antibodies  >6 publications with SLIT2 and Disease Models, Animal
 Small Cell Lung Carcinoma Antibodies  >6 publications with SLIT2 and Small Cell Lung Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >72 publications with SLIT2 and Brain
 Embryonic Tissue Antibodies  >51 publications with SLIT2 and Embryonic Tissue
 Eye Antibodies  >28 publications with SLIT2 and Eye
 Vascular Antibodies  >24 publications with SLIT2 and Vascular
 Lung Antibodies  >17 publications with SLIT2 and Lung
 Kidney Antibodies  >14 publications with SLIT2 and Kidney
 Ganglia Antibodies  >10 publications with SLIT2 and Ganglia
 Muscle Antibodies  >6 publications with SLIT2 and Muscle
 Liver Antibodies  >5 publications with SLIT2 and Liver
 Prostate Antibodies  >5 publications with SLIT2 and Prostate
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