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SLC11A1 elisa kit :: Human solute carrier family 11 (proton-coupled divalent metal ion transporters) , member 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS939156
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

solute carrier family 11 (proton-coupled divalent metal ion transporters) , member 1 (SLC11A1), ELISA Kit

★Popular Item★
 Also Known As   

Human natural resistance-associated macrophage protein 1, NRAMP-1 ELISA Kit

 Product Synonym Names    Human natural resistance-associated macrophage protein 1 (NRAMP-1) ELISA kit; LSH; NRAMP; NRAMP1; natural resistance-associated macrophage protein 1; solute carrier family 11 (sodium/phosphate symporters); member 1; solute carrier family 11 (proton-coupled divalent metal ion transporters); member 1
 Product Gene Name   

SLC11A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS939156 Testing Data    Testing Data PDF
 OMIM    610446
 3D Structure    ModBase 3D Structure for P49279
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NRAMP-1. No significant cross-reactivity or interference between human NRAMP-1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml -1600 pg/ml.
 Sensitivity    The minimum detectable dose of human NRAMP-1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SLC11A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SLC11A1 purchase    MBS939156 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing SLC11A1, with an estimated sensitivity of The minimum detectable dose of human NRAMP-1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS939156 kit is based on SLC11A1 antibody-SLC11A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC11A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC11A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SLC11A1 elisa kit

  
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRAMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRAMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRAMP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRAMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SLC11A1 elisa kit    SLC11A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS939156. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SLC11A1. It may not necessarily be applicable to this product.
 NCBI GI #    109255241
 NCBI GeneID    6556
 NCBI Accession #    NP_000569.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000578.3 [Other Products]
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 UniProt Primary Accession #    P49279 [Other Products]
 UniProt Secondary Accession #    C0H5Y3 [Other Products]
 UniProt Related Accession #    P49279 [Other Products]
 Molecular Weight    59,872 Da
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 NCBI Official Full Name    natural resistance-associated macrophage protein 1
 NCBI Official Synonym Full Names    solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1
 NCBI Official Symbol    SLC11A1 [Similar Products]
 NCBI Official Synonym Symbols   
LSH; NRAMP; NRAMP1
[Similar Products]
 NCBI Protein Information    natural resistance-associated macrophage protein 1; NRAMP 1; solute carrier family 11 member 1; solute carrier family 11 (sodium/phosphate symporters), member 1; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
 UniProt Protein Name    Natural resistance-associated macrophage protein 1
 UniProt Synonym Protein Names   
Solute carrier family 11 member 1
 Protein Family    Natural resistance-associated macrophage protein
 UniProt Gene Name    SLC11A1 [Similar Products]
 UniProt Synonym Gene Names    LSH; NRAMP; NRAMP1; NRAMP 1 [Similar Products]
 UniProt Entry Name    NRAM1_HUMAN
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 NCBI Summary for SLC11A1    This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SLC11A1    SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Vesicle; Cell surface; Transporter

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: phagocytic vesicle membrane; lysosome; integral to plasma membrane; late endosome membrane; late endosome; plasma membrane

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; positive regulation of T-helper 1 type immune response; wound healing; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; vacuolar acidification; antigen processing and presentation of peptide antigen; nitrite transport; T cell proliferation during immune response; T cell cytokine production; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; transmembrane transport; positive regulation of cytokine production; interaction with host; mRNA stabilization; L-arginine import; defense response to protozoan; interleukin-2 production; iron ion transport; phagocytosis; macrophage activation; cellular cadmium ion homeostasis; iron ion homeostasis; MHC class II biosynthetic process; positive regulation of interferon-gamma production; response to bacterium; protein import into nucleus, translocation; defense response to bacterium; negative regulation of cytokine production; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter

Disease: Buruli Ulcer, Susceptibility To; Mycobacterium Tuberculosis, Susceptibility To
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 Research Articles on SLC11A1    1. Polymorphism of the NRAMP1 gene was investigated by PCR amplification followed by RFLP analysis... patients with heterozygosity of intron 4 (GC) and/or maternal infection with helminth parasites showed reduced efficacy of BCG vaccine against tuberculosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SLC11A1 elisa kitDiseases associated with SLC11A1 elisa kit
 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metal Ion SLC Transporters Pathway antibodies  Metal Ion SLC Transporters Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway antibodies  Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies  Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram
 Disease Name  Pubmed Publications
 Tuberculosis Antibodies  >40 publications with SLC11A1 and Tuberculosis
 MYCOBACTERIUM TUBERCULOSIS, SUSCEPTIBILITY TO Antibodies  >16 publications with SLC11A1 and MYCOBACTERIUM TUBERCULOSIS, SUSCEPTIBILITY TO
 Respiratory Tract Infections Antibodies  >15 publications with SLC11A1 and Respiratory Tract Infections
 Necrosis Antibodies  >13 publications with SLC11A1 and Necrosis
 Disease Models, Animal Antibodies  >12 publications with SLC11A1 and Disease Models, Animal
 Inflammatory Bowel Diseases Antibodies  >8 publications with SLC11A1 and Inflammatory Bowel Diseases
 Diabetes Mellitus, Type 1 Antibodies  >8 publications with SLC11A1 and Diabetes Mellitus, Type 1
 Arthritis, Rheumatoid Antibodies  >7 publications with SLC11A1 and Arthritis, Rheumatoid
 Leishmaniasis, Visceral Antibodies  >6 publications with SLC11A1 and Leishmaniasis, Visceral
 Colitis, Ulcerative Antibodies  >5 publications with SLC11A1 and Colitis, Ulcerative
Organs/Tissues associated with SLC11A1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with SLC11A1 and Blood
 Spleen Antibodies  >13 publications with SLC11A1 and Spleen
 Liver Antibodies  >8 publications with SLC11A1 and Liver
 Intestine Antibodies  >6 publications with SLC11A1 and Intestine
 Lung Antibodies  >6 publications with SLC11A1 and Lung
 Bone Marrow Antibodies  >5 publications with SLC11A1 and Bone Marrow
 Lymph Node Antibodies  >5 publications with SLC11A1 and Lymph Node
 Brain Antibodies  >3 publications with SLC11A1 and Brain
 Kidney Antibodies  >2 publications with SLC11A1 and Kidney
 Pancreas Antibodies  >1 publications with SLC11A1 and Pancreas
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