Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow SHH rightarrow Human SHH  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Free cholesterol (FCHOL) ELISA Kit
 • BD-2 (SAP1) (BD-2 (SAP1)) Recombinant Protein
 • Matrix Metalloproteinase 9 (MMP-9) ELISA Kit
 • Cyclomaltodextrin glucanotransferase, partial (CGTase) Recombinant Protein
 • 5'-Nucleotidase, Ecto (NT5E) ELISA Kit
 • Mouse Serum, Filtered Reagent
 • myeloid differentiation primary response gene (88) (MYD88) ELISA Kit
 • Tissue factor pathway inhibitor (TFPI) ELISA Kit
 • Biotin Conjugated Goat Anti-mouse IgG Secondary Antibody
 • AKT1 Antibody
 • DDT Antibody
 • burgdorferi IgM ELISA Kit
 • Salmonellae sp. Antibody
 • BrdU Cell Proliferation Assay Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

SHH elisa kit :: Human sonic hedgehog homolog (Drosophila) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702035
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

sonic hedgehog homolog (Drosophila) (SHH), ELISA Kit

★Popular Item★
 Also Known As   

Human sonic hedgehog ELISA Kit

 Product Synonym Names    Human sonic hedgehog ELISA kit; HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog; sonic hedgehog homolog (Drosophila)
 Product Gene Name   

SHH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702035 Testing Data    Testing Data PDF
 OMIM    gene 611638
 3D Structure    ModBase 3D Structure for Q15465
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of human sonic hedgehog. No significant cross-reactivity or interference between human sonic hedgehog and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml -4000 pg/ml.
 Sensitivity    The minimum detectable dose of human sonic hedgehog is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SHH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SHH purchase    MBS702035 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sonic hedgehog homolog (Drosophila) (SHH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml -4000 pg/ml in biological research samples containing SHH, with an estimated sensitivity of The minimum detectable dose of human sonic hedgehog is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS702035 kit is based on SHH antibody-SHH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SHH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SHH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for SHH elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sonic hedgehog has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sonic hedgehog present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sonic hedgehog is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sonic hedgehog bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of SHH elisa kit    SHH elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS702035. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for SHH. It may not necessarily be applicable to this product.
 NCBI GI #    4506939
 NCBI GeneID    6469
 NCBI Accession #    NP_000184.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000193.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q15465 [Other Products]
 UniProt Secondary Accession #    Q75MC9; A4D247 [Other Products]
 UniProt Related Accession #    Q15465 [Other Products]
 Molecular Weight    49,607 Da
Table BarTOPTable Bar
 NCBI Official Full Name    sonic hedgehog protein preproprotein
 NCBI Official Synonym Full Names    sonic hedgehog
 NCBI Official Symbol    SHH [Similar Products]
 NCBI Official Synonym Symbols   
TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
[Similar Products]
 NCBI Protein Information    sonic hedgehog protein; sonic hedgehog homolog
 UniProt Protein Name    Sonic hedgehog protein
 UniProt Synonym Protein Names   
HHG-1Cleaved into the following 2 chains:Sonic hedgehog protein N-product; Sonic hedgehog protein C-product
 Protein Family    Sonic hedgehog protein
 UniProt Gene Name    SHH [Similar Products]
 UniProt Synonym Gene Names    SHH [Similar Products]
 UniProt Entry Name    SHH_HUMAN
Section Bar
 NCBI Summary for SHH    This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for SHH    Function: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO 1 PublicationManual assertion based on experiment in:Ref.11
Table BarTOPTable Bar
 Research Articles on SHH    1. Low expression of Sonic hedgehog signaling proteins is related to lepidic predominant subtype in primary lung adenocarcinoma.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with SHH elisa kitPathways associated with SHH elisa kit
 Reference Product  PubMed Publications
 GLI1 elisa kit  >531 publications with SHH and GLI1
 GLI3 elisa kit  >293 publications with SHH and GLI3
 GLI2 elisa kit  >287 publications with SHH and GLI2
 BMP4 elisa kit  >244 publications with SHH and BMP4
 PTCH1 elisa kit  >198 publications with SHH and PTCH1
 HHIP elisa kit  >41 publications with SHH and HHIP
 GAS1 elisa kit  >26 publications with SHH and GAS1
 PTCH2 elisa kit  >24 publications with SHH and PTCH2
 GPC3 elisa kit  >7 publications with SHH and GPC3
 LRP2 elisa kit  >4 publications with SHH and LRP2
 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Glypican 3 Network Pathway antibodies  Glypican 3 Network Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Ligand Biogenesis Pathway antibodies  Hedgehog Ligand Biogenesis Pathway Diagram
Diseases associated with SHH elisa kitOrgans/Tissues associated with SHH elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >550 publications with SHH and Nervous System Diseases
 Brain Diseases Antibodies  >301 publications with SHH and Brain Diseases
 Abnormalities, Multiple Antibodies  >299 publications with SHH and Abnormalities, Multiple
 Craniofacial Abnormalities Antibodies  >228 publications with SHH and Craniofacial Abnormalities
 Disease Models, Animal Antibodies  >209 publications with SHH and Disease Models, Animal
 Holoprosencephaly Antibodies  >192 publications with SHH and Holoprosencephaly
 Adenocarcinoma Antibodies  >124 publications with SHH and Adenocarcinoma
 Skin Diseases Antibodies  >112 publications with SHH and Skin Diseases
 Neural Tube Defects Antibodies  >104 publications with SHH and Neural Tube Defects
 Holoprosencephaly 3 Antibodies  >77 publications with SHH and Holoprosencephaly 3
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >795 publications with SHH and Embryonic Tissue
 Brain Antibodies  >781 publications with SHH and Brain
 Skin Antibodies  >207 publications with SHH and Skin
 Lung Antibodies  >142 publications with SHH and Lung
 Prostate Antibodies  >74 publications with SHH and Prostate
 Liver Antibodies  >60 publications with SHH and Liver
 Molar Antibodies  >35 publications with SHH and Molar
 Bladder Antibodies  >26 publications with SHH and Bladder
 Adrenal Gland Antibodies  >13 publications with SHH and Adrenal Gland
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions