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SPAG9 elisa kit :: Human sperm associated antigen 9 (SPAG9) antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703947
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

sperm associated antigen 9 (SPAG9) antibody, ELISA Kit

★Popular Item★
 Also Known As   

Human sperm associated antigen 9, SPAG9 antibody ELISA Kit

 Product Synonym Names    Human sperm associated antigen 9 (SPAG9) antibody ELISA Kit; sperm associated antigen 9 (SPAG9) antibody
 Product Gene Name   

SPAG9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703947 Testing Data    Testing Data PDF
 OMIM    605430
 3D Structure    ModBase 3D Structure for O60271
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human SPAG9 antibody. No significant cross-reactivity or interference between human SPAG9 antibody and analogues was observed.
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 Samples    Serum, plasma
 Assay Type    Sandwich
 Detection Range    50 mU/ml-1200 mU/ml.
 Sensitivity    The minimum detectable dose of human SPAG9 antibody is typically less than 20 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human SPAG9 antibody concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SPAG9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SPAG9 purchase    MBS703947 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sperm associated antigen 9 (SPAG9) antibody, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 mU/ml-1200 mU/ml in biological research samples containing SPAG9, with an estimated sensitivity of The minimum detectable dose of human SPAG9 antibody is typically less than 20 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human SPAG9 antibody concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS703947 kit is based on SPAG9 antibody-SPAG9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPAG9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPAG9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). For the detection of the human product of NBCI gene JLP, the scaffolding leucine zipper containing sperm antigen SPAG 9, also named Jun amino terminal kinase interacting protein 4, a precision ELISA kit has been developed and they show excellent dynamic range in serum samples. These ELISA kits are ideal for research involving spermatogenesis, cell proliferation or cancer signaling pathways. As a JNK interacting protein, dysregulation of MAPK signaling is a direct result in loss of or enhanced expression of SPAG9. The sandwich format assay kit utilizes human specific antibody products developed by Gentaur against human sperm antigen 9. Available as strips or a 96 well plate, assay kits contain a standard protein and stop solution reagent. Details on the SPAG9 customize antibody ELISA kit and appropriate uses for individual antibodies may be requested from mybiosource.com.
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Product Description specifically for SPAG9 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SPAG9. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated SPAG9. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPAG9 antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SPAG9 elisa kit    SPAG9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703947. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SPAG9. It may not necessarily be applicable to this product.
 NCBI GI #    158253415
 NCBI GeneID    9043
 NCBI Accession #    AAI53879.1 [Other Products]
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 UniProt Primary Accession #    O60271 [Other Products]
 UniProt Secondary Accession #    O60905; Q3KQU8; Q3MKM7; Q86WC7; Q86WC8; Q8IZX7; Q96II0; Q9H811; A6H8U5; A8MSX0; B4DHH2 [Other Products]
 UniProt Related Accession #    O60271 [Other Products]
 Molecular Weight    128,607 Da [Similar Products]
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 NCBI Official Full Name    SPAG9 protein
 NCBI Official Synonym Full Names    sperm associated antigen 9
 NCBI Official Symbol    SPAG9 [Similar Products]
 NCBI Official Synonym Symbols   
JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
[Similar Products]
 NCBI Protein Information    C-Jun-amino-terminal kinase-interacting protein 4; sunday driver 1; Max-binding protein; sperm surface protein; lung cancer oncogene 4; JNK interacting protein; cancer/testis antigen 89; JNK/SAPK-associated protein; proliferation-inducing gene 6; protein highly expressed in testis; human lung cancer oncogene 6 protein; sperm associated antigen 9 variant 5; mitogen-activated protein kinase 8-interacting protein 4; c-Jun NH2-terminal kinase-associated leucine zipper protein
 UniProt Protein Name    C-Jun-amino-terminal kinase-interacting protein 4
 UniProt Synonym Protein Names   
Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1
 Protein Family    C-Jun-amino-terminal kinase-interacting protein
 UniProt Gene Name    SPAG9 [Similar Products]
 UniProt Synonym Gene Names    HSS; KIAA0516; MAPK8IP4; SYD1; HLC6; JIP-4; JNK-interacting protein 4; CT89; HLC-6; JLP; PHET [Similar Products]
 UniProt Entry Name    JIP4_HUMAN
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 NCBI Summary for SPAG9    This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]
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 UniProt Comments for SPAG9    Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction.2 PublicationsManual assertion based on experiment in:Ref.11
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 Research Articles on SPAG9    1. SPAG9 depletion could inhibit the activity of p-JNK.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SPAG9 elisa kitPathways associated with SPAG9 elisa kit
 Reference Product  PubMed Publications
 MYC elisa kit  >1 publications with SPAG9 and MYC
 MAX elisa kit  >1 publications with SPAG9 and MAX
 MAPK14 elisa kit  >1 publications with SPAG9 and MAPK14
 MAP3K3 elisa kit  >1 publications with SPAG9 and MAP3K3
 MAP2K4 elisa kit  >1 publications with SPAG9 and MAP2K4
 KLC1 elisa kit  >1 publications with SPAG9 and KLC1
 Products by Pathway  Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 CDO In Myogenesis Pathway antibodies  CDO In Myogenesis Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Myogenesis Pathway antibodies  Myogenesis Pathway Diagram
Diseases associated with SPAG9 elisa kitOrgans/Tissues associated with SPAG9 elisa kit
 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >5 publications with SPAG9 and Ovarian Neoplasms
 Carcinoma, Squamous Cell Antibodies  >3 publications with SPAG9 and Carcinoma, Squamous Cell
 Nervous System Diseases Antibodies  >2 publications with SPAG9 and Nervous System Diseases
 Breast Neoplasms Antibodies  >2 publications with SPAG9 and Breast Neoplasms
 Brain Diseases Antibodies  >2 publications with SPAG9 and Brain Diseases
 Hyperplasia Antibodies  >2 publications with SPAG9 and Hyperplasia
 Nerve Degeneration Antibodies  >1 publications with SPAG9 and Nerve Degeneration
 Fibrosis Antibodies  >1 publications with SPAG9 and Fibrosis
 Lung Neoplasms Antibodies  >1 publications with SPAG9 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with SPAG9 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >14 publications with SPAG9 and Testis
 Blood Antibodies  >7 publications with SPAG9 and Blood
 Nerve Antibodies  >6 publications with SPAG9 and Nerve
 Muscle Antibodies  >3 publications with SPAG9 and Muscle
 Lung Antibodies  >3 publications with SPAG9 and Lung
 Skin Antibodies  >2 publications with SPAG9 and Skin
 Embryonic Tissue Antibodies  >2 publications with SPAG9 and Embryonic Tissue
 Kidney Antibodies  >2 publications with SPAG9 and Kidney
 Brain Antibodies  >2 publications with SPAG9 and Brain
 Prostate Antibodies  >1 publications with SPAG9 and Prostate
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