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| Product Name
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sperm autoantigenic protein 17 (SPA17), ELISA Kit ★Popular Item★
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| Also Known As
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Human Sperm surface protein Sp17, SPA17 ELISA Kit
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| Product Synonym Names
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Human Sperm surface protein Sp17 (SPA17) ELISA kit; CT22; SP17; SP17-1; cancer/testis antigen 22; sperm protein 17; sperm autoantigenic protein 17
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS933873 Testing Data
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| OMIM
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608621
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| 3D Structure
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ModBase 3D Structure for Q15506
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| Species Reactivity
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Human
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of human SPA17. No significant cross-reactivity or interference between human SPA17 and analogues was observed.
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| Samples
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Serum, plasma, tissue homogenates, cell lysates.
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| Assay Type
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Sandwich
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| Detection Range
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64 pg/ml-2400 pg/ml.
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| Sensitivity
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Typically less than 16 pg/ml
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
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| Inter-assay Precision
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Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
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| Preparation and Storage
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of SPA17 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for SPA17 purchase
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MBS933873 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sperm autoantigenic protein 17 (SPA17) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 64 pg/ml-2400 pg/ml in biological research samples containing SPA17, with an estimated sensitivity of Typically less than 16 pg/ml. The ELISA analytical biochemical technique of the MBS933873 kit is based on SPA17 antibody-SPA17 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPA17 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPA17. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SPA17 elisa kit
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPA17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for SPA17. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPA17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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| Typical Testing Data/Standard Curve (for reference only) of SPA17 elisa kit
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| Sample Manual Insert of MBS933873. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for SPA17. It may not necessarily be applicable to this product. |
| NCBI GI #
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8394343
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| NCBI GeneID
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53340
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| NCBI Accession #
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NP_059121.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_017425.3
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[Other Products]
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| UniProt Primary Accession #
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Q15506
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[Other Products]
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| UniProt Secondary Accession #
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Q9BXF7; B2R4F2
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[Other Products]
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| UniProt Related Accession #
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Q15506
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[Other Products]
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| Molecular Weight
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17,406 Da
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| NCBI Official Full Name
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sperm surface protein Sp17
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| NCBI Official Synonym Full Names
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sperm autoantigenic protein 17
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| NCBI Official Symbol
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SPA17
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[Similar Products]
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| NCBI Official Synonym Symbols
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CT22; SP17; SP17-1
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[Similar Products]
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| NCBI Protein Information
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sperm surface protein Sp17; sperm protein 17; cancer/testis antigen 22
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| UniProt Protein Name
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Sperm surface protein Sp17
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| UniProt Synonym Protein Names
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Cancer/testis antigen 22; CT22; Sp17-1; Sperm autoantigenic protein 17; Sperm protein 17
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| Protein Family
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Sperm surface protein
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| UniProt Gene Name
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SPA17
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[Similar Products]
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| UniProt Synonym Gene Names
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SP17; CT22; Sperm protein 17
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[Similar Products]
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| UniProt Entry Name
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SP17_HUMAN
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| NCBI Summary for SPA17
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This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]
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| UniProt Comments for SPA17
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SPA17: Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: membrane; cytoplasm
Molecular Function: calmodulin binding
Biological Process: binding of sperm to zona pellucida; single fertilization; spermatogenesis
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| Research Articles on SPA17
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1. NY-ESO-1 and SP17 was not significantly associated with a specific histotype, but high-level GAGE expression was more frequent in squamous cell carcinoma. GAGE expression was demonstrated to be significantly higher in stage II-IIIa than stage I NSCLC.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with SPA17 elisa kit | Diseases associated with SPA17 elisa kit |
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| Organs/Tissues associated with SPA17 elisa kit |
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ELISA Kit 
