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SPA17 elisa kit :: Human sperm autoantigenic protein 17 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS933873
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

sperm autoantigenic protein 17 (SPA17), ELISA Kit

★Popular Item★
 Also Known As   

Human Sperm surface protein Sp17, SPA17 ELISA Kit

 Product Synonym Names    Human Sperm surface protein Sp17 (SPA17) ELISA kit; CT22; SP17; SP17-1; cancer/testis antigen 22; sperm protein 17; sperm autoantigenic protein 17
 Product Gene Name   

SPA17 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS933873 Testing Data    Testing Data PDF
 OMIM    608621
 3D Structure    ModBase 3D Structure for Q15506
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human SPA17. No significant cross-reactivity or interference between human SPA17 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich
 Detection Range    64 pg/ml-2400 pg/ml.
 Sensitivity    Typically less than 16 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SPA17 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SPA17 purchase    MBS933873 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sperm autoantigenic protein 17 (SPA17) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 64 pg/ml-2400 pg/ml in biological research samples containing SPA17, with an estimated sensitivity of Typically less than 16 pg/ml. The ELISA analytical biochemical technique of the MBS933873 kit is based on SPA17 antibody-SPA17 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPA17 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPA17. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SPA17 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPA17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for SPA17. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPA17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SPA17 elisa kit    SPA17 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS933873. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SPA17. It may not necessarily be applicable to this product.
 NCBI GI #    8394343
 NCBI GeneID    53340
 NCBI Accession #    NP_059121.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_017425.3 [Other Products]
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 UniProt Primary Accession #    Q15506 [Other Products]
 UniProt Secondary Accession #    Q9BXF7; B2R4F2 [Other Products]
 UniProt Related Accession #    Q15506 [Other Products]
 Molecular Weight    17,406 Da
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 NCBI Official Full Name    sperm surface protein Sp17
 NCBI Official Synonym Full Names    sperm autoantigenic protein 17
 NCBI Official Symbol    SPA17 [Similar Products]
 NCBI Official Synonym Symbols   
CT22; SP17; SP17-1
[Similar Products]
 NCBI Protein Information    sperm surface protein Sp17; sperm protein 17; cancer/testis antigen 22
 UniProt Protein Name    Sperm surface protein Sp17
 UniProt Synonym Protein Names   
Cancer/testis antigen 22; CT22; Sp17-1; Sperm autoantigenic protein 17; Sperm protein 17
 Protein Family    Sperm surface protein
 UniProt Gene Name    SPA17 [Similar Products]
 UniProt Synonym Gene Names    SP17; CT22; Sperm protein 17 [Similar Products]
 UniProt Entry Name    SP17_HUMAN
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 NCBI Summary for SPA17    This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]
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 UniProt Comments for SPA17    SPA17: Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: membrane; cytoplasm

Molecular Function: calmodulin binding

Biological Process: binding of sperm to zona pellucida; single fertilization; spermatogenesis
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 Research Articles on SPA17    1. NY-ESO-1 and SP17 was not significantly associated with a specific histotype, but high-level GAGE expression was more frequent in squamous cell carcinoma. GAGE expression was demonstrated to be significantly higher in stage II-IIIa than stage I NSCLC.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SPA17 elisa kitDiseases associated with SPA17 elisa kit
 Reference Product  PubMed Publications
 ROPN1 elisa kit  >2 publications with SPA17 and ROPN1
 ACRBP elisa kit  >1 publications with SPA17 and ACRBP
 ZPBP elisa kit  >1 publications with SPA17 and ZPBP
 ROPN1L elisa kit  >1 publications with SPA17 and ROPN1L
 AKAP3 elisa kit  >1 publications with SPA17 and AKAP3
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >10 publications with SPA17 and Cardiovascular Diseases
 Genital Diseases, Male Antibodies  >4 publications with SPA17 and Genital Diseases, Male
 Liver Neoplasms Antibodies  >4 publications with SPA17 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3 publications with SPA17 and Carcinoma, Hepatocellular
 Infertility, Male Antibodies  >2 publications with SPA17 and Infertility, Male
 Infertility, Female Antibodies  >2 publications with SPA17 and Infertility, Female
 Liver Diseases Antibodies  >2 publications with SPA17 and Liver Diseases
 Lung Neoplasms Antibodies  >2 publications with SPA17 and Lung Neoplasms
 Inflammation Antibodies  >2 publications with SPA17 and Inflammation
 Nervous System Diseases Antibodies  >2 publications with SPA17 and Nervous System Diseases
Organs/Tissues associated with SPA17 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >28 publications with SPA17 and Testis
 Liver Antibodies  >3 publications with SPA17 and Liver
 Lung Antibodies  >3 publications with SPA17 and Lung
 Ovary Antibodies  >3 publications with SPA17 and Ovary
 Trachea Antibodies  >2 publications with SPA17 and Trachea
 Embryonic Tissue Antibodies  >2 publications with SPA17 and Embryonic Tissue
 Kidney Antibodies  >2 publications with SPA17 and Kidney
 Ascites Antibodies  >2 publications with SPA17 and Ascites
 Esophagus Antibodies  >1 publications with SPA17 and Esophagus
 Prostate Antibodies  >1 publications with SPA17 and Prostate
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