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SMPD1 elisa kit :: Human sphingomyelin phosphodiesterase 1, acid lysosomal ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700800
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), ELISA Kit

 Also Known As   

Human acid sphingomyelinase, ASM ELISA Kit

 Product Synonym Names    Human acid sphingomyelinase; ASM ELISA Kit; ASM; NPD; acid sphingomyelinase; sphingomyelin phosphodiesterase 1; acid lysosomal
 Product Gene Name   

SMPD1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700800 Testing Data    Testing Data PDF
 OMIM    gene 607616
 3D Structure    ModBase 3D Structure for P17405
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human SMPD1. No significant cross-reactivity or interference between Human SMPD1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, cerebrospinal fluid (CSF) and urine
 Detection Range    1.25 ng/ml-80 ng/ml
 Sensitivity    0.31 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SMPD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SMPD1 purchase    MBS700800 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.25 ng/ml-80 ng/ml in biological research samples containing SMPD1, with an estimated sensitivity of 0.31 ng/ml. The ELISA analytical biochemical technique of the MBS700800 kit is based on SMPD1 antibody-SMPD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SMPD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SMPD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, cerebrospinal fluid (CSF) and urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SMPD1 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ASM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ASM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ASM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ASM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of SMPD1 elisa kit    SMPD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700800. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SMPD1. It may not necessarily be applicable to this product.
 NCBI GI #    56117840
 NCBI GeneID    6609
 NCBI Accession #    NP_000534.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000543.4 [Other Products]
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 UniProt Primary Accession #    P17405 [Other Products]
 UniProt Secondary Accession #    P17406; Q13811; Q16837; Q16841; A8K8M3; E9PKS3 [Other Products]
 UniProt Related Accession #    P17405 [Other Products]
 Molecular Weight    69,752 Da
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 NCBI Official Full Name    sphingomyelin phosphodiesterase isoform 1
 NCBI Official Synonym Full Names    sphingomyelin phosphodiesterase 1, acid lysosomal
 NCBI Official Symbol    SMPD1 [Similar Products]
 NCBI Official Synonym Symbols   
ASM; NPD; ASMASE
[Similar Products]
 NCBI Protein Information    sphingomyelin phosphodiesterase; acid sphingomyelinase
 UniProt Protein Name    Sphingomyelin phosphodiesterase
 UniProt Synonym Protein Names   
Acid sphingomyelinase; aSMase
 Protein Family    Sphingomyelin phosphodiesterase
 UniProt Gene Name    SMPD1 [Similar Products]
 UniProt Synonym Gene Names    ASM; aSMase [Similar Products]
 UniProt Entry Name    ASM_HUMAN
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 NCBI Summary for SMPD1    The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
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 UniProt Comments for SMPD1    Function: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity.

Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.
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 Research Articles on SMPD1    1. The lysosomal enzyme coding genes SMPD1 which are associated with a 9-fold increased risk of Parkinson disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SMPD1 elisa kitPathways associated with SMPD1 elisa kit
 Reference Product  PubMed Publications
 ASAH1 elisa kit  >61 publications with SMPD1 and ASAH1
 ASAH2 elisa kit  >53 publications with SMPD1 and ASAH2
 FADD elisa kit  >10 publications with SMPD1 and FADD
 FASLG elisa kit  >6 publications with SMPD1 and FASLG
 SMPD2 elisa kit  >3 publications with SMPD1 and SMPD2
 GALC elisa kit  >2 publications with SMPD1 and GALC
 GBA elisa kit  >2 publications with SMPD1 and GBA
 TNFRSF1A elisa kit  >2 publications with SMPD1 and TNFRSF1A
 CERK elisa kit  >1 publications with SMPD1 and CERK
 UGCG elisa kit  >1 publications with SMPD1 and UGCG
 Products by Pathway  Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 FAS (CD95) Signaling Pathway antibodies  FAS (CD95) Signaling Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 IL2 Signaling Events Mediated By PI3K Pathway antibodies  IL2 Signaling Events Mediated By PI3K Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Ovarian Infertility Genes Pathway antibodies  Ovarian Infertility Genes Pathway Diagram
Diseases associated with SMPD1 elisa kitOrgans/Tissues associated with SMPD1 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >453 publications with SMPD1 and Nervous System Diseases
 Brain Diseases Antibodies  >432 publications with SMPD1 and Brain Diseases
 Niemann-Pick Diseases Antibodies  >368 publications with SMPD1 and Niemann-Pick Diseases
 Necrosis Antibodies  >313 publications with SMPD1 and Necrosis
 Disease Models, Animal Antibodies  >133 publications with SMPD1 and Disease Models, Animal
 Liver Diseases Antibodies  >127 publications with SMPD1 and Liver Diseases
 Inflammation Antibodies  >125 publications with SMPD1 and Inflammation
 Cardiovascular Diseases Antibodies  >106 publications with SMPD1 and Cardiovascular Diseases
 Niemann-Pick Disease, Type B Antibodies  >37 publications with SMPD1 and Niemann-Pick Disease, Type B
 Fibrosis Antibodies  >32 publications with SMPD1 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with SMPD1 and Bone
 Connective Tissue Antibodies  >4 publications with SMPD1 and Connective Tissue
 Heart Antibodies  >3 publications with SMPD1 and Heart
 Muscle Antibodies  >3 publications with SMPD1 and Muscle
 Brain Antibodies  >3 publications with SMPD1 and Brain
 Vascular Antibodies  >2 publications with SMPD1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with SMPD1 and Embryonic Tissue
 Ear Antibodies  >1 publications with SMPD1 and Ear
 Kidney Antibodies  >1 publications with SMPD1 and Kidney
 Umbilical Cord Antibodies  >1 publications with SMPD1 and Umbilical Cord
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