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SYNJ2 elisa kit :: Human synaptojanin 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703853
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

synaptojanin 2 (SYNJ2), ELISA Kit

★Popular Item★
 Also Known As   

Human Synaptojanin 2, SYNJ2 ELISA Kit

 Product Synonym Names    Human Synaptojanin 2 (SYNJ2) ELISA Kit; RP11-266C7.2; INPP5H; KIAA0348; MGC44422; inositol phosphate 5 -phosphatase 2 (synaptojanin 2) ; synaptojanin 2
 Product Gene Name   

SYNJ2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703853 Testing Data    Testing Data PDF
 OMIM    609410
 3D Structure    ModBase 3D Structure for O15056
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human SYNJ2. No significant cross-reactivity or interference between Human SYNJ2 and analogues was observed.
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 Detection Range    156.25 pg/ml-10000 pg/ml
 Sensitivity    40 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SYNJ2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SYNJ2 purchase    MBS703853 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the synaptojanin 2 (SYNJ2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 156.25 pg/ml-10000 pg/ml in biological research samples containing SYNJ2, with an estimated sensitivity of 40 pg/ml. The ELISA analytical biochemical technique of the MBS703853 kit is based on SYNJ2 antibody-SYNJ2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SYNJ2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SYNJ2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SYNJ2 elisa kit

   Introduction: Synaptojanin 2 (SYNJ2) is a ubiquitously expressed inositol 5 phosphatase, which may be involved in distinct membrane trafficking and signal transduction pathways. Synaptojanin 2 is a Rac1 effector and may mediate the inhibitory effect of Rac1 on clathrin mediated endocytosis.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SYNJ2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SYNJ2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SYNJ2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SYNJ2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of SYNJ2 elisa kit    SYNJ2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703853. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SYNJ2. It may not necessarily be applicable to this product.
 NCBI GI #    26190608
 NCBI GeneID    8871
 NCBI Accession #    NP_003889.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003898.3 [Other Products]
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 UniProt Primary Accession #    O15056 [Other Products]
 UniProt Secondary Accession #    Q5TA13; Q5TA16; Q5TA19; Q86XK0; Q8IZA8; Q9H226 [Other Products]
 UniProt Related Accession #    O15056 [Other Products]
 Molecular Weight    165,538 Da
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 NCBI Official Full Name    synaptojanin-2 isoform 1
 NCBI Official Synonym Full Names    synaptojanin 2
 NCBI Official Symbol    SYNJ2 [Similar Products]
 NCBI Official Synonym Symbols   
INPP5H
[Similar Products]
 NCBI Protein Information    synaptojanin-2; inositol phosphate 5'-phosphatase 2; synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2; synaptic inositol-1,4,5-trisphosphate 5-phosphatase 2
 UniProt Protein Name    Synaptojanin-2
 UniProt Synonym Protein Names   
Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2
 Protein Family    Synaptojanin
 UniProt Gene Name    SYNJ2 [Similar Products]
 UniProt Synonym Gene Names    KIAA0348 [Similar Products]
 UniProt Entry Name    SYNJ2_HUMAN
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 NCBI Summary for SYNJ2    The gene is a member of the inositol-polyphosphate 5-phosphatase family. The encoded protein interacts with the ras-related C3 botulinum toxin substrate 1, which causes translocation of the encoded protein to the plasma membrane where it inhibits clathrin-mediated endocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
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 UniProt Comments for SYNJ2    Function: Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis.

Catalytic activity: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
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 Research Articles on SYNJ2    1. The genetic interactions associated with ILVatrophy rate in this study may be mapping variants inSYNJ2andPI4KAthat interact to decrease synthesisof PIP.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SYNJ2 elisa kitPathways associated with SYNJ2 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with SYNJ2 and FLNA
 Products by Pathway  Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
Diseases associated with SYNJ2 elisa kitOrgans/Tissues associated with SYNJ2 elisa kit
 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >1 publications with SYNJ2 and Prostatic Neoplasms
 Cognition Disorders Antibodies  >1 publications with SYNJ2 and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with SYNJ2 and Bone
 Connective Tissue Antibodies  >4 publications with SYNJ2 and Connective Tissue
 Heart Antibodies  >3 publications with SYNJ2 and Heart
 Muscle Antibodies  >3 publications with SYNJ2 and Muscle
 Brain Antibodies  >3 publications with SYNJ2 and Brain
 Vascular Antibodies  >2 publications with SYNJ2 and Vascular
 Embryonic Tissue Antibodies  >2 publications with SYNJ2 and Embryonic Tissue
 Ear Antibodies  >1 publications with SYNJ2 and Ear
 Kidney Antibodies  >1 publications with SYNJ2 and Kidney
 Umbilical Cord Antibodies  >1 publications with SYNJ2 and Umbilical Cord
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