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TPMT elisa kit :: Human thiopurine S-methyltransferase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS938845
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
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10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

thiopurine S-methyltransferase (TPMT), ELISA Kit

★Popular Item★
 Also Known As   

Human thiopurine S-methyltransferase, TPMT ELISA Kit

 Product Synonym Names    Human thiopurine S-methyltransferase (TPMT) ELISA kit; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine S-methyltransferase
 Product Gene Name   

TPMT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS938845 COA    COA PDF
 MBS938845 Testing Data    Testing Data PDF
 OMIM    gene 610460
 3D Structure    ModBase 3D Structure for P51580
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human TPMT. No significant cross-reactivity or interference between human TPMT and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    1.56 mU/ml -100 mU/ml
 Sensitivity    Typically less than 0.39 mU/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPMT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPMT purchase    MBS938845 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the thiopurine S-methyltransferase (TPMT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 mU/ml -100 mU/ml in biological research samples containing TPMT, with an estimated sensitivity of Typically less than 0.39 mU/ml. The ELISA analytical biochemical technique of the MBS938845 kit is based on TPMT antibody-TPMT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPMT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPMT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TPMT elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TPMT elisa kit    TPMT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS938845. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPMT. It may not necessarily be applicable to this product.
 NCBI GI #    4507653
 NCBI GeneID    7172
 NCBI Accession #    NP_000358.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000367.3 [Other Products]
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 UniProt Primary Accession #    P51580 [Other Products]
 UniProt Secondary Accession #    O14806; O15423; O15424; O15425; O15426; O15515; O15548; O43213; Q5VUK6; Q9UBE6; Q9UBT8 [Other Products]
 UniProt Related Accession #    P51580 [Other Products]
 Molecular Weight    28,180 Da
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 NCBI Official Full Name    thiopurine S-methyltransferase
 NCBI Official Synonym Full Names    thiopurine S-methyltransferase
 NCBI Official Symbol    TPMT [Similar Products]
 NCBI Protein Information    thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase
 UniProt Protein Name    Thiopurine S-methyltransferase
 UniProt Synonym Protein Names   
Thiopurine methyltransferase
 Protein Family    Thiopurine S-methyltransferase
 UniProt Gene Name    TPMT [Similar Products]
 UniProt Entry Name    TPMT_HUMAN
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 NCBI Summary for TPMT    This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]
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 UniProt Comments for TPMT    TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family.

Protein type: Methyltransferase; EC 2.1.1.67; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytosol

Molecular Function: thiopurine S-methyltransferase activity

Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Disease: Thiopurine S-methyltransferase Deficiency
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 Research Articles on TPMT    1. Activity measurement performed at diagnosis provides clinicians with information on immediate pharmacokinetic-related adverse events and/or hypermetabolism
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TPMT elisa kitDiseases associated with TPMT elisa kit
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Methylation Pathway antibodies  Methylation Pathway Diagram
 Phase II Conjugation Pathway antibodies  Phase II Conjugation Pathway Diagram
 Disease Name  Pubmed Publications
 Drug Toxicity Antibodies  >173 publications with TPMT and Drug Toxicity
 Gastrointestinal Diseases Antibodies  >150 publications with TPMT and Gastrointestinal Diseases
 Neoplasms Antibodies  >109 publications with TPMT and Neoplasms
 THIOPURINE S-METHYLTRANSFERASE DEFICIENCY Antibodies  >102 publications with TPMT and THIOPURINE S-METHYLTRANSFERASE DEFICIENCY
 Leukemia, Lymphoid Antibodies  >72 publications with TPMT and Leukemia, Lymphoid
 Hematologic Diseases Antibodies  >70 publications with TPMT and Hematologic Diseases
 Precursor Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >69 publications with TPMT and Precursor Cell Lymphoblastic Leukemia-Lymphoma
 Leukopenia Antibodies  >55 publications with TPMT and Leukopenia
 Crohn Disease Antibodies  >52 publications with TPMT and Crohn Disease
 Liver Diseases Antibodies  >36 publications with TPMT and Liver Diseases
Organs/Tissues associated with TPMT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >266 publications with TPMT and Blood
 Bone Antibodies  >62 publications with TPMT and Bone
 Bone Marrow Antibodies  >61 publications with TPMT and Bone Marrow
 Liver Antibodies  >59 publications with TPMT and Liver
 Kidney Antibodies  >42 publications with TPMT and Kidney
 Skin Antibodies  >10 publications with TPMT and Skin
 Heart Antibodies  >6 publications with TPMT and Heart
 Intestine Antibodies  >3 publications with TPMT and Intestine
 Lung Antibodies  >3 publications with TPMT and Lung
 Eye Antibodies  >3 publications with TPMT and Eye
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