Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow TMEFF2 rightarrow Human TMEFF2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • HEN1-2 Antibody
 • Ribonuclease H (RNASEH) ELISA Kit
 • Ankyrin B (ANK-B) ELISA Kit
 • Serine Protease (PRSS) ELISA Kit
 • Inducible Nitric Oxide Synthase (INOS) ELISA Kit
 • Cytochrome B5 (CYTB5) ELISA Kit
 • Embryonic Stem Mespu30 (M30) ELISA Kit
 • Serum Amyloid P Component (SAP) ELISA Kit
 • Transcription Factor SOX-4 (SOX4) ELISA Kit
 • Neurotrophin-4 (NT-4) ELISA Kit
 • Jo-1 Antibody (JO1) ELISA Kit
 • Galactose 6-Sulphate Sulphatase (GAL-6S) ELISA Kit
 • Chemokine Interferon-Inducible Protein 4 (IP-4) ELISA Kit
 • High Mobility Group Nucleosome Binding Domain 1 (HMGN1) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

TMEFF2 elisa kit :: Human transmembrane protein with EGF-like and two follistatin-like domains 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS928793
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

transmembrane protein with EGF-like and two follistatin-like domains 2 (TMEFF2), ELISA Kit

 Also Known As   

Human Tomoregulin-2, TMEFF2 ELISA Kit

 Product Synonym Names    Human Tomoregulin-2 (TMEFF2) ELISA kit; HPP1; TENB2; TPEF; TR; tomoregulin; transmembrane protein TENB2; transmembrane protein with EGF-like and two follistatin-like domains 2
 Product Gene Name   

TMEFF2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS928793 Testing Data    Testing Data PDF
 OMIM    605734
 3D Structure    ModBase 3D Structure for Q9UIK5
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of human TMEFF2. No significant cross-reactivity or interference between human TMEFF2 and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    28 pg/ml -1800 pg/ml.
 Sensitivity    The minimum detectable dose of human TMEFF2 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TMEFF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TMEFF2 purchase    MBS928793 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transmembrane protein with EGF-like and two follistatin-like domains 2 (TMEFF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 28 pg/ml -1800 pg/ml in biological research samples containing TMEFF2, with an estimated sensitivity of The minimum detectable dose of human TMEFF2 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS928793 kit is based on TMEFF2 antibody-TMEFF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TMEFF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TMEFF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for TMEFF2 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TMEFF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEFF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of TMEFF2 elisa kit    TMEFF2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS928793. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for TMEFF2. It may not necessarily be applicable to this product.
 NCBI GI #    12383051
 NCBI GeneID    23671
 NCBI Accession #    NP_057276.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_016192.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q9UIK5 [Other Products]
 UniProt Secondary Accession #    Q2FA44; Q4ZFW4; Q53H90; Q53RE1; Q8N2R5; Q9NR15; Q9NSS5; Q9P2Y9; Q9UK65 [Other Products]
 UniProt Related Accession #    Q9UIK5 [Other Products]
 Molecular Weight    41,428 Da
Table BarTOPTable Bar
 NCBI Official Full Name    tomoregulin-2
 NCBI Official Synonym Full Names    transmembrane protein with EGF-like and two follistatin-like domains 2
 NCBI Official Symbol    TMEFF2 [Similar Products]
 NCBI Official Synonym Symbols   
TR; HPP1; TPEF; TENB2; CT120.2
[Similar Products]
 NCBI Protein Information    tomoregulin-2; TR-2; transmembrane protein TENB2; hyperplastic polyposis protein 1; cancer/testis antigen family 120, member 2
 UniProt Protein Name    Tomoregulin-2
 UniProt Synonym Protein Names   
Hyperplastic polyposis protein 1; Transmembrane protein with EGF-like and two follistatin-like domains
 Protein Family    Tomoregulin
 UniProt Gene Name    TMEFF2 [Similar Products]
 UniProt Synonym Gene Names    HPP1; TENB2; TPEF; UNQ178/PRO204; TR-2 [Similar Products]
 UniProt Entry Name    TEFF2_HUMAN
Section Bar
 NCBI Summary for TMEFF2    This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Section Bar
 UniProt Comments for TMEFF2    TMEFF2: may be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. A member of the Cancer-Testis Antigen (CTA) superfamily. CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region. The CpG island methylation correlates with TMEFF2 silencing in tumor cell lines. Belongs to the tomoregulin family. Three isoforms of the human protein are produced by alternative splicing. Isoform three is expressed weakly in testis and at high levels in normal and cancerous prostate. Note: This description may include information from RefSeq and UniProtKB

Protein type: Cancer Testis Antigen (CTA); Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 2q32.3

Cellular Component: integral to membrane; extracellular region
Table BarTOPTable Bar
 Research Articles on TMEFF2    1. TMEFF2 and SARDH cooperate to modulate one-carbon metabolism and invasion of prostate cancer cells.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with TMEFF2 elisa kitDiseases associated with TMEFF2 elisa kit
 Products by Pathway  Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies  Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >53 publications with TMEFF2 and Neoplasms
 Intestinal Diseases Antibodies  >15 publications with TMEFF2 and Intestinal Diseases
 Adenocarcinoma Antibodies  >13 publications with TMEFF2 and Adenocarcinoma
 Hyperplasia Antibodies  >6 publications with TMEFF2 and Hyperplasia
 Esophageal Neoplasms Antibodies  >6 publications with TMEFF2 and Esophageal Neoplasms
 Lung Neoplasms Antibodies  >6 publications with TMEFF2 and Lung Neoplasms
 Stomach Neoplasms Antibodies  >6 publications with TMEFF2 and Stomach Neoplasms
 Neoplasm Metastasis Antibodies  >5 publications with TMEFF2 and Neoplasm Metastasis
 Carcinoma, Squamous Cell Antibodies  >5 publications with TMEFF2 and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >3 publications with TMEFF2 and Brain Diseases
Organs/Tissues associated with TMEFF2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >16 publications with TMEFF2 and Prostate
 Brain Antibodies  >12 publications with TMEFF2 and Brain
 Lung Antibodies  >6 publications with TMEFF2 and Lung
 Nerve Antibodies  >5 publications with TMEFF2 and Nerve
 Embryonic Tissue Antibodies  >3 publications with TMEFF2 and Embryonic Tissue
 Intestine Antibodies  >2 publications with TMEFF2 and Intestine
 Bone Antibodies  >2 publications with TMEFF2 and Bone
 Eye Antibodies  >1 publications with TMEFF2 and Eye
 Kidney Antibodies  >1 publications with TMEFF2 and Kidney
 Uterus Antibodies  >1 publications with TMEFF2 and Uterus
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions