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TFF1 elisa kit :: Human trefoil factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702209
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

trefoil factor 1 (TFF1), ELISA Kit

★Popular Item★
 Also Known As   

Human Estrogen-induced protein pS2, PS2 ELISA Kit

 Product Synonym Names    Human Estrogen-induced protein pS2 (PS2) ELISA Kit; BCEI; D21S21; HP1.A; HPS2; pNR-2; pS2; breast cancer estrogen-inducible sequence; gastrointestinal trefoil protein pS2; trefoil factor 1
 Product Gene Name   

TFF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702209 Testing Data    Testing Data PDF
 OMIM    113710
 3D Structure    ModBase 3D Structure for P04155
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human TFF1. No significant cross-reactivity or interference between Human TFF1 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Assay Type    Competitive
 Detection Range    3.2 ng/ml-80 ng/ml
 Sensitivity    2 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TFF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TFF1 purchase    MBS702209 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the trefoil factor 1 (TFF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.2 ng/ml-80 ng/ml in biological research samples containing TFF1, with an estimated sensitivity of 2 ng/ml. The ELISA analytical biochemical technique of the MBS702209 kit is based on TFF1 antibody-TFF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TFF1 elisa kit

   Introduction: The estrogen-induced protein pS2 is secreted by MCF7 cells upon stimulation by estrogen. Estrogen mediates the transcription of responsive genes via its interaction with the estrogen receptor (ER). ps2 gene is the most interesting predictor for researching the anti-estrogen therapy of breast cancer. But the biological function and role of ps2 is not yet very clear. Now somebody think it has a certain specificity of breast cancer. At the same time, ps2 can reflect the expression of tumor differentiation.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A anti-antibody specific to the antibody of pS2 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for pS2 and Horseradish Peroxidase (HRP) conjugated pS2, then incubated. A competitive inhibition reaction is launched between pS2 (Standards or samples) and Horseradish Peroxidase (HRP) conjugated pS2 with the antibody specific for pS2. The more the amount of pS2 in samples, the less antibody bound by Horseradish Peroxidase (HRP) conjugated pS2. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of pS2 in the sample. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TFF1 elisa kit    TFF1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702209. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TFF1. It may not necessarily be applicable to this product.
 NCBI GI #    4507451
 NCBI GeneID    7031
 NCBI Accession #    NP_003216.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003225.2 [Other Products]
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 UniProt Primary Accession #    P04155 [Other Products]
 UniProt Related Accession #    P04155 [Other Products]
 Molecular Weight    9,150 Da
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 NCBI Official Full Name    trefoil factor 1
 NCBI Official Synonym Full Names    trefoil factor 1
 NCBI Official Symbol    TFF1 [Similar Products]
 NCBI Official Synonym Symbols   
pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
[Similar Products]
 NCBI Protein Information    trefoil factor 1; protein pS2; polypeptide P1.A; gastrointestinal trefoil protein pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence
 UniProt Protein Name    Trefoil factor 1
 UniProt Synonym Protein Names   
Breast cancer estrogen-inducible protein; PNR-2; Polypeptide P1.A; hP1.A; Protein pS2
 Protein Family    Trefoil factor
 UniProt Gene Name    TFF1 [Similar Products]
 UniProt Synonym Gene Names    BCEI; PS2; hP1.A [Similar Products]
 UniProt Entry Name    TFF1_HUMAN
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 NCBI Summary for TFF1    Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TFF1    Function: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.1 PublicationManual assertion based on experiment in:Ref.11
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 Research Articles on TFF1    1. Piezo1 is a novel TFF1 binding protein that is important for TFF1-mediated cell migration in gastric cancer cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TFF1 elisa kitPathways associated with TFF1 elisa kit
 Reference Product  PubMed Publications
 FOXA1 elisa kit  >13 publications with TFF1 and FOXA1
 MUC5B elisa kit  >11 publications with TFF1 and MUC5B
 ESR1 elisa kit  >9 publications with TFF1 and ESR1
 PCNA elisa kit  >7 publications with TFF1 and PCNA
 GKN2 elisa kit  >7 publications with TFF1 and GKN2
 NCOA1 elisa kit  >3 publications with TFF1 and NCOA1
 NCOA3 elisa kit  >3 publications with TFF1 and NCOA3
 NCOA2 elisa kit  >2 publications with TFF1 and NCOA2
 Products by Pathway  Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
Diseases associated with TFF1 elisa kitOrgans/Tissues associated with TFF1 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >404 publications with TFF1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >134 publications with TFF1 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >73 publications with TFF1 and Neoplasm Metastasis
 Inflammation Antibodies  >41 publications with TFF1 and Inflammation
 Neoplasm Invasiveness Antibodies  >37 publications with TFF1 and Neoplasm Invasiveness
 Hyperplasia Antibodies  >34 publications with TFF1 and Hyperplasia
 Neoplasms, Experimental Antibodies  >30 publications with TFF1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >22 publications with TFF1 and Cell Transformation, Neoplastic
 Precancerous Conditions Antibodies  >18 publications with TFF1 and Precancerous Conditions
 Lung Neoplasms Antibodies  >17 publications with TFF1 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >162 publications with TFF1 and Stomach
 Intestine Antibodies  >71 publications with TFF1 and Intestine
 Blood Antibodies  >52 publications with TFF1 and Blood
 Prostate Antibodies  >21 publications with TFF1 and Prostate
 Pancreas Antibodies  >18 publications with TFF1 and Pancreas
 Brain Antibodies  >13 publications with TFF1 and Brain
 Mammary Gland Antibodies  >9 publications with TFF1 and Mammary Gland
 Ovary Antibodies  >7 publications with TFF1 and Ovary
 Uncharacterized Tissue Antibodies  >1 publications with TFF1 and Uncharacterized Tissue
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