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TRIB3 elisa kit :: Human tribbles homolog 3 (Drosophila) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS932141
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tribbles homolog 3 (Drosophila) (TRIB3), ELISA Kit

★Popular Item★
 Also Known As   

Human Tribbles homolog 3, TRIB3 ELISA Kit

 Product Synonym Names    Human Tribbles homolog 3 (TRIB3) ELISA kit; RP5-1103G7.7; C20orf97; NIPK; SINK; SKIP3; TRB3; neuronal cell death inducible putative kinase; p65-interacting inhibitor of NF-kappaB; tribbles 3; tribbles homolog 3 (Drosophila)
 Product Gene Name   

TRIB3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS932141 Testing Data    Testing Data PDF
 OMIM    607898
 3D Structure    ModBase 3D Structure for Q96RU7
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human TRIB3. No significant cross-reactivity or interference between human TRIB3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    18.75 pg/ml -1200 pg/ml.
 Sensitivity    The minimum detectable dose of human TRIB3 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TRIB3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TRIB3 purchase    MBS932141 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tribbles homolog 3 (Drosophila) (TRIB3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.75 pg/ml -1200 pg/ml in biological research samples containing TRIB3, with an estimated sensitivity of The minimum detectable dose of human TRIB3 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS932141 kit is based on TRIB3 antibody-TRIB3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRIB3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRIB3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TRIB3 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRIB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIB3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TRIB3 elisa kit    TRIB3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS932141. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TRIB3. It may not necessarily be applicable to this product.
 NCBI GI #    31542265
 NCBI GeneID    57761
 NCBI Accession #    NP_066981.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_021158.4 [Other Products]
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 UniProt Primary Accession #    Q96RU7 [Other Products]
 UniProt Secondary Accession #    Q53GU4; Q53ZW7; Q6I9Y9; Q8TAI6; Q9H5M8; Q9NUD2 [Other Products]
 UniProt Related Accession #    Q96RU7 [Other Products]
 Molecular Weight    39,578 Da
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 NCBI Official Full Name    tribbles homolog 3 isoform 1
 NCBI Official Synonym Full Names    tribbles pseudokinase 3
 NCBI Official Symbol    TRIB3 [Similar Products]
 NCBI Official Synonym Symbols   
NIPK; SINK; TRB3; SKIP3; C20orf97
[Similar Products]
 NCBI Protein Information    tribbles homolog 3; TRB-3; p65-interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; neuronal cell death inducible putative kinase; neuronal cell death-inducible putative kinase
 UniProt Protein Name    Tribbles homolog 3
 UniProt Synonym Protein Names   
Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B
 Protein Family    Tribbles
 UniProt Gene Name    TRIB3 [Similar Products]
 UniProt Synonym Gene Names    C20orf97; NIPK; SKIP3; TRB3; TRB-3 [Similar Products]
 UniProt Entry Name    TRIB3_HUMAN
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 NCBI Summary for TRIB3    The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
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 UniProt Comments for TRIB3    TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.

Protein type: Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 20p13-p12.2

Cellular Component: nucleoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein binding; protein kinase inhibitor activity; ubiquitin-protein ligase regulator activity; ubiquitin protein ligase binding; kinase activity; transcription corepressor activity; protein kinase binding; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of protein binding; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of ubiquitin-protein ligase activity; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process
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 Research Articles on TRIB3    1. The correlation between TRB3 and Notch 1 expression revealed that Notch 1 was downregulated by the knockdown of TRB3 in the lung adenocarcinoma cell lines.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TRIB3 elisa kitPathways associated with TRIB3 elisa kit
 Reference Product  PubMed Publications
 ATF4 elisa kit  >28 publications with TRIB3 and ATF4
 AKT1 elisa kit  >4 publications with TRIB3 and AKT1
 AKT2 elisa kit  >2 publications with TRIB3 and AKT2
 Products by Pathway  Pathway Diagram
 Activation Of PKB Pathway antibodies  Activation Of PKB Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
Diseases associated with TRIB3 elisa kitOrgans/Tissues associated with TRIB3 elisa kit
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >37 publications with TRIB3 and Insulin Resistance
 Cardiovascular Diseases Antibodies  >19 publications with TRIB3 and Cardiovascular Diseases
 Inflammation Antibodies  >10 publications with TRIB3 and Inflammation
 Breast Neoplasms Antibodies  >8 publications with TRIB3 and Breast Neoplasms
 Necrosis Antibodies  >8 publications with TRIB3 and Necrosis
 Liver Diseases Antibodies  >8 publications with TRIB3 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >6 publications with TRIB3 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >6 publications with TRIB3 and Liver Neoplasms
 Heart Diseases Antibodies  >5 publications with TRIB3 and Heart Diseases
 Nervous System Diseases Antibodies  >4 publications with TRIB3 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >19 publications with TRIB3 and Blood
 Muscle Antibodies  >18 publications with TRIB3 and Muscle
 Liver Antibodies  >17 publications with TRIB3 and Liver
 Vascular Antibodies  >6 publications with TRIB3 and Vascular
 Pancreas Antibodies  >6 publications with TRIB3 and Pancreas
 Bone Antibodies  >6 publications with TRIB3 and Bone
 Kidney Antibodies  >5 publications with TRIB3 and Kidney
 Bone Marrow Antibodies  >3 publications with TRIB3 and Bone Marrow
 Connective Tissue Antibodies  >2 publications with TRIB3 and Connective Tissue
 Lung Antibodies  >2 publications with TRIB3 and Lung
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