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TPI1 elisa kit :: Human triosephosphate isomerase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS901437
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

triosephosphate isomerase 1 (TPI1), ELISA Kit

★Popular Item★
 Also Known As   

Human Triosephosphate isomerase, TPI1 ELISA Kit

 Product Synonym Names    Human Triosephosphate isomerase (TPI1) ELISA kit; MGC88108; TPI; ; triosephosphate isomerase 1
 Product Gene Name   

TPI1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS901437 Testing Data    Testing Data PDF
 OMIM    gene 615512
 3D Structure    ModBase 3D Structure for P60174
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human TPI1. No significant cross-reactivity or interference between human TPI1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich
 Detection Range    28 pg/ml -1800 pg/ml.
 Sensitivity    7 pg /ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPI1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPI1 purchase    MBS901437 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the triosephosphate isomerase 1 (TPI1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 28 pg/ml -1800 pg/ml in biological research samples containing TPI1, with an estimated sensitivity of 7 pg /ml. The ELISA analytical biochemical technique of the MBS901437 kit is based on TPI1 antibody-TPI1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPI1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPI1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TPI1 elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TPI1 elisa kit    TPI1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS901437. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPI1. It may not necessarily be applicable to this product.
 NCBI GI #    4507645
 NCBI GeneID    7167
 NCBI Accession #    NP_000356.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000365.5 [Other Products]
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 UniProt Primary Accession #    P60174 [Other Products]
 UniProt Secondary Accession #    P00938; Q6FHP9; Q6IS07; Q8WWD0; Q96AG5; B7Z5D8; D3DUS9 [Other Products]
 UniProt Related Accession #    P60174 [Other Products]
 Molecular Weight    30,791 Da
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 NCBI Official Full Name    triosephosphate isomerase isoform 1
 NCBI Official Synonym Full Names    triosephosphate isomerase 1
 NCBI Official Symbol    TPI1 [Similar Products]
 NCBI Official Synonym Symbols   
TIM; TPI; TPID; HEL-S-49
[Similar Products]
 NCBI Protein Information    triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49
 UniProt Protein Name    Triosephosphate isomerase
 UniProt Synonym Protein Names   
Triose-phosphate isomerase
 Protein Family    Triosephosphate isomerase
 UniProt Gene Name    TPI1 [Similar Products]
 UniProt Synonym Gene Names    TPI; TIM [Similar Products]
 UniProt Entry Name    TPIS_HUMAN
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 NCBI Summary for TPI1    This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
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 UniProt Comments for TPI1    TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular space; cytosol; nucleus

Molecular Function: protein binding; triose-phosphate isomerase activity

Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

Disease: Triosephosphate Isomerase Deficiency
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 Research Articles on TPI1    1. E104D mutant is highly susceptible to proteolysis, which in all likelihood contributes to the pathogenesis of enzymopathy. In addition, the proteolysis data on wild type HsTIM illustrate an asymmetric conduct of the two monomers.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TPI1 elisa kitDiseases associated with TPI1 elisa kit
 Products by Pathway  Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7 publications with TPI1 and Neoplasms
 Breast Neoplasms Antibodies  >3 publications with TPI1 and Breast Neoplasms
 Skin Diseases Antibodies  >3 publications with TPI1 and Skin Diseases
 Disease Models, Animal Antibodies  >3 publications with TPI1 and Disease Models, Animal
 Adenocarcinoma Antibodies  >3 publications with TPI1 and Adenocarcinoma
 Hypertension Antibodies  >2 publications with TPI1 and Hypertension
 Drug Toxicity Antibodies  >1 publications with TPI1 and Drug Toxicity
 Liver Neoplasms Antibodies  >1 publications with TPI1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with TPI1 and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >1 publications with TPI1 and Liver Diseases
Organs/Tissues associated with TPI1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with TPI1 and Blood
 Brain Antibodies  >3 publications with TPI1 and Brain
 Prostate Antibodies  >2 publications with TPI1 and Prostate
 Muscle Antibodies  >2 publications with TPI1 and Muscle
 Kidney Antibodies  >2 publications with TPI1 and Kidney
 Mouth Antibodies  >1 publications with TPI1 and Mouth
 Connective Tissue Antibodies  >1 publications with TPI1 and Connective Tissue
 Heart Antibodies  >1 publications with TPI1 and Heart
 Liver Antibodies  >1 publications with TPI1 and Liver
 Adipose Tissue Antibodies  >1 publications with TPI1 and Adipose Tissue
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