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TRIM72 elisa kit :: Human tripartite motif-containing 72 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS943592
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tripartite motif-containing 72 (TRIM72), ELISA Kit

★Popular Item★
 Also Known As   

Human Tripartite motif-containing protein 72, TRIM72 ELISA Kit

 Product Synonym Names    Human Tripartite motif-containing protein 72 (TRIM72) ELISA kit; MG53; mitsugumin-53; tripartite motif-containing 72
 Product Gene Name   

TRIM72 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS943592 Testing Data    Testing Data PDF
 OMIM    613288
 3D Structure    ModBase 3D Structure for Q6ZMU5
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human TRIM72. No significant cross-reactivity or interference between human TRIM72 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    25 pg/ml -1600 pg/ml
 Sensitivity    Typically less than 6.25 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TRIM72 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TRIM72 purchase    MBS943592 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tripartite motif-containing 72 (TRIM72) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing TRIM72, with an estimated sensitivity of Typically less than 6.25 pg/ml. The ELISA analytical biochemical technique of the MBS943592 kit is based on TRIM72 antibody-TRIM72 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRIM72 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRIM72. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TRIM72 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRIM72 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIM72 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM72 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM72 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TRIM72 elisa kit    TRIM72 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS943592. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TRIM72. It may not necessarily be applicable to this product.
 NCBI GI #    270265876
 NCBI GeneID    493829
 NCBI Accession #    NP_001008275.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001008274.3 [Other Products]
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 UniProt Primary Accession #    Q6ZMU5 [Other Products]
 UniProt Secondary Accession #    Q8N4X6; Q8NBD9 [Other Products]
 UniProt Related Accession #    Q6ZMU5 [Other Products]
 Molecular Weight    52,731 Da
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 NCBI Official Full Name    tripartite motif-containing protein 72
 NCBI Official Synonym Full Names    tripartite motif containing 72, E3 ubiquitin protein ligase
 NCBI Official Symbol    TRIM72 [Similar Products]
 NCBI Official Synonym Symbols   
MG53
[Similar Products]
 NCBI Protein Information    tripartite motif-containing protein 72; mitsugumin 53; mitsugumin-53; tripartite motif-containing 72
 UniProt Protein Name    Tripartite motif-containing protein 72
 UniProt Synonym Protein Names   
Mitsugumin-53; Mg53
 Protein Family    Tripartite motif-containing protein
 UniProt Gene Name    TRIM72 [Similar Products]
 UniProt Synonym Gene Names    MG53; Mg53 [Similar Products]
 UniProt Entry Name    TRI72_HUMAN
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 UniProt Comments for TRIM72    TRIM72: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasmic vesicle membrane; sarcolemma

Molecular Function: zinc ion binding; phosphatidylserine binding

Biological Process: muscle development; exocytosis; plasma membrane repair; muscle system process; protein homooligomerization
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 Research Articles on TRIM72    1. MG53 induces FAK ubiquitination with the aid of UBE2H during skeletal myogenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with TRIM72 elisa kitOrgans/Tissues associated with TRIM72 elisa kit
 Disease Name  Pubmed Publications
 Musculoskeletal Diseases Antibodies  >1 publications with TRIM72 and Musculoskeletal Diseases
 Hypospadias Antibodies  >1 publications with TRIM72 and Hypospadias
 Syndrome Antibodies  >1 publications with TRIM72 and Syndrome
 Hypertelorism Antibodies  >1 publications with TRIM72 and Hypertelorism
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >7 publications with TRIM72 and Muscle
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