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TPM4 elisa kit :: Human tropomyosin 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS944560
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tropomyosin 4 (TPM4), ELISA Kit

★Popular Item★
 Also Known As   

Human Tropomyosin alpha-4 chain, TPM4 ELISA Kit

 Product Synonym Names    Human Tropomyosin alpha-4 chain (TPM4) ELISA kit; tropomyosin 4
 Product Gene Name   

TPM4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS944560 COA    COA PDF
 MBS944560 Testing Data    Testing Data PDF
 OMIM    600317
 3D Structure    ModBase 3D Structure for P67936
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human TPM4. No significant cross-reactivity or interference between human TPM4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    23.5 pg/ml -1500 pg/ml
 Sensitivity    Typically less than 5.8 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPM4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPM4 purchase    MBS944560 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tropomyosin 4 (TPM4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.5 pg/ml -1500 pg/ml in biological research samples containing TPM4, with an estimated sensitivity of Typically less than 5.8 pg/ml. The ELISA analytical biochemical technique of the MBS944560 kit is based on TPM4 antibody-TPM4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPM4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPM4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TPM4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPM4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TPM4 elisa kit    TPM4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS944560. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPM4. It may not necessarily be applicable to this product.
 NCBI GI #    223555975
 NCBI GeneID    7171
 NCBI Accession #    NP_001138632.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145160.1 [Other Products]
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 UniProt Primary Accession #    P67936 [Other Products]
 UniProt Secondary Accession #    P07226; Q15659; Q5U0D9; Q9BU85; Q9H8Q3; Q9UCS1; Q9UCS2; Q9UCS3; Q9UCS4 [Other Products]
 UniProt Related Accession #    P67936 [Other Products]
 Molecular Weight    28,522 Da
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 NCBI Official Full Name    tropomyosin alpha-4 chain isoform 1
 NCBI Official Synonym Full Names    tropomyosin 4
 NCBI Official Symbol    TPM4 [Similar Products]
 NCBI Official Synonym Symbols   
HEL-S-108
[Similar Products]
 NCBI Protein Information    tropomyosin alpha-4 chain; TM30p1; tropomyosin-4; epididymis secretory protein Li 108
 UniProt Protein Name    Tropomyosin alpha-4 chain
 UniProt Synonym Protein Names   
TM30p1; Tropomyosin-4
 Protein Family    Tropomyosin
 UniProt Gene Name    TPM4 [Similar Products]
 UniProt Entry Name    TPM4_HUMAN
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 NCBI Summary for TPM4    This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]
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 UniProt Comments for TPM4    TPM4: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In nonmuscle cells is implicated in stabilizing cytoskeleton actin filaments and endocytosis.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cortical cytoskeleton; filamentous actin; cytoskeleton; focal adhesion; membrane; stress fiber; podosome; cytosol; muscle thin filament tropomyosin

Molecular Function: structural constituent of muscle; calcium ion binding; actin binding

Biological Process: osteoblast differentiation; muscle contraction; cell motility; muscle filament sliding
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 Research Articles on TPM4    1. In addition to CLIC1 and TPM1, which were the proteins initially discovered in a xenograft mouse model, CLIC4, TPM2, TPM3, and TPM4 were present in ovarian cancer patient sera at significantly elevated levels compared with controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TPM4 elisa kitDiseases associated with TPM4 elisa kit
 Products by Pathway  Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Smooth Muscle Contraction Pathway antibodies  Smooth Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >16 publications with TPM4 and Neoplasms
 Carcinoma Antibodies  >7 publications with TPM4 and Carcinoma
 Esophageal Neoplasms Antibodies  >4 publications with TPM4 and Esophageal Neoplasms
 Carcinoma, Squamous Cell Antibodies  >4 publications with TPM4 and Carcinoma, Squamous Cell
 Breast Neoplasms Antibodies  >3 publications with TPM4 and Breast Neoplasms
 Neoplasm Invasiveness Antibodies  >2 publications with TPM4 and Neoplasm Invasiveness
 Lung Neoplasms Antibodies  >2 publications with TPM4 and Lung Neoplasms
 Inflammation Antibodies  >2 publications with TPM4 and Inflammation
 Cardiovascular Diseases Antibodies  >2 publications with TPM4 and Cardiovascular Diseases
 Heart Failure Antibodies  >2 publications with TPM4 and Heart Failure
Organs/Tissues associated with TPM4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >14 publications with TPM4 and Muscle
 Heart Antibodies  >8 publications with TPM4 and Heart
 Lung Antibodies  >5 publications with TPM4 and Lung
 Blood Antibodies  >4 publications with TPM4 and Blood
 Embryonic Tissue Antibodies  >4 publications with TPM4 and Embryonic Tissue
 Vascular Antibodies  >3 publications with TPM4 and Vascular
 Esophagus Antibodies  >2 publications with TPM4 and Esophagus
 Ovary Antibodies  >1 publications with TPM4 and Ovary
 Placenta Antibodies  >1 publications with TPM4 and Placenta
 Liver Antibodies  >1 publications with TPM4 and Liver
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