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TNNT3 elisa kit :: Human troponin T type 3 (skeletal, fast) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS928285
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

troponin T type 3 (skeletal, fast) (TNNT3), ELISA Kit

★Popular Item★
 Also Known As   

Human Troponin T, fast skeletal muscle, TNNT3 ELISA Kit

 Product Synonym Names    Human Troponin T; fast skeletal muscle (TNNT3) ELISA kit; DKFZp779M2348; TNTF; OTTHUMP00000014435; troponin T; fast skeletal muscle; troponin T3; skeletal; fast; troponin-T3; skeletal; fast; troponin T type 3 (skeletal; fast)
 Product Gene Name   

TNNT3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS928285 Testing Data    Testing Data PDF
 OMIM    gene 601680
 3D Structure    ModBase 3D Structure for P45378
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human TNNT3. No significant cross-reactivity or interference between human TNNT3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    7.8 1 pg/ml -500 pg/ml.
 Sensitivity    The minimum detectable dose of human TNNT3 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNNT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNNT3 purchase    MBS928285 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the troponin T type 3 (skeletal, fast) (TNNT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 1 pg/ml -500 pg/ml in biological research samples containing TNNT3, with an estimated sensitivity of The minimum detectable dose of human TNNT3 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS928285 kit is based on TNNT3 antibody-TNNT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNNT3 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNNT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TNNT3 elisa kit    TNNT3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS928285. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNNT3. It may not necessarily be applicable to this product.
 NCBI GI #    112789536
 NCBI GeneID    7140
 NCBI Accession #    NP_001036245.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001042780.2 [Other Products]
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 UniProt Primary Accession #    P45378 [Other Products]
 UniProt Secondary Accession #    Q12975; Q12976; Q12977; Q12978; Q17RG9; A8MQ76; A8MSW1; B3KPX3; B7WP64; B7ZL26; B7ZVV9 [Other Products]
 UniProt Related Accession #    P45378 [Other Products]
 Molecular Weight    31,825 Da
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 NCBI Official Full Name    troponin T, fast skeletal muscle isoform 3
 NCBI Official Synonym Full Names    troponin T type 3 (skeletal, fast)
 NCBI Official Symbol    TNNT3 [Similar Products]
 NCBI Official Synonym Symbols   
TNTF
[Similar Products]
 NCBI Protein Information    troponin T, fast skeletal muscle; fTnT; beta TnTF; beta-TnTF; troponin-T3, skeletal, fast; fast skeletal muscle troponin T
 UniProt Protein Name    Troponin T, fast skeletal muscle
 UniProt Synonym Protein Names   
Beta-TnTF; Fast skeletal muscle troponin T; fTnT
 Protein Family    Troponin
 UniProt Gene Name    TNNT3 [Similar Products]
 UniProt Synonym Gene Names    TnTf; fTnT [Similar Products]
 UniProt Entry Name    TNNT3_HUMAN
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 NCBI Summary for TNNT3    The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009]
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 UniProt Comments for TNNT3    TNNT3: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin T family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: troponin complex; cytosol

Molecular Function: troponin C binding; troponin I binding; actin binding; tropomyosin binding; calcium-dependent protein binding; calcium-dependent ATPase activity

Biological Process: skeletal muscle contraction; regulation of ATPase activity; metabolic process; regulation of striated muscle contraction; muscle filament sliding

Disease: Arthrogryposis, Distal, Type 2b
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 Research Articles on TNNT3    1. Data show that a missense mutation at nucleotide position 187 in exon 9 of the TNNT3 gene was identified in all affected individuals in the family.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNNT3 elisa kitPathways associated with TNNT3 elisa kit
 Reference Product  PubMed Publications
 TNNI2 elisa kit  >12 publications with TNNT3 and TNNI2
 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with TNNT3 elisa kitOrgans/Tissues associated with TNNT3 elisa kit
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >12 publications with TNNT3 and Congenital Abnormalities
 Musculoskeletal Abnormalities Antibodies  >10 publications with TNNT3 and Musculoskeletal Abnormalities
 Nervous System Diseases Antibodies  >5 publications with TNNT3 and Nervous System Diseases
 Distal arthrogryposis type 2B Antibodies  >3 publications with TNNT3 and Distal arthrogryposis type 2B
 Skin Diseases Antibodies  >1 publications with TNNT3 and Skin Diseases
 Hypertension Antibodies  >1 publications with TNNT3 and Hypertension
 Atrophy Antibodies  >1 publications with TNNT3 and Atrophy
 Kidney Diseases Antibodies  >1 publications with TNNT3 and Kidney Diseases
 Fetal Diseases Antibodies  >1 publications with TNNT3 and Fetal Diseases
 Eye Abnormalities Antibodies  >1 publications with TNNT3 and Eye Abnormalities
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >21 publications with TNNT3 and Muscle
 Heart Antibodies  >4 publications with TNNT3 and Heart
 Eye Antibodies  >2 publications with TNNT3 and Eye
 Lung Antibodies  >2 publications with TNNT3 and Lung
 Nerve Antibodies  >1 publications with TNNT3 and Nerve
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