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TPH1 elisa kit :: Human tryptophan hydroxylase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702096
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tryptophan hydroxylase 1 (TPH1), ELISA Kit

★Popular Item★
 Also Known As   

Human tryptophan hydroxylase, TPH ELISA Kit

 Product Synonym Names    Human tryptophan hydroxylase (TPH) ELISA Kit; MGC119994; TPRH; TRPH; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase) ; tryptophan hydroxylase 1
 Product Gene Name   

TPH1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702096 Testing Data    Testing Data PDF
 OMIM    191060
 3D Structure    ModBase 3D Structure for P17752
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human TPH1. No significant cross-reactivity or interference between Human TPH1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Detection Range    34.4 pg/ml-2200 pg/ml
 Sensitivity    8.6 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Neurobiology
 Protein Biological Process 3    Serotonin biosynthesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPH1 purchase    MBS702096 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tryptophan hydroxylase 1 (TPH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 34.4 pg/ml-2200 pg/ml in biological research samples containing TPH1, with an estimated sensitivity of 8.6 pg/ml. The ELISA analytical biochemical technique of the MBS702096 kit is based on TPH1 antibody-TPH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TPH1 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated TPH and antibody preparation specific for TPH and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TPH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TPH1 elisa kit    TPH1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702096. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPH1. It may not necessarily be applicable to this product.
 NCBI GI #    4759248
 NCBI GeneID    7166
 NCBI Accession #    NP_004170.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004179.2 [Other Products]
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 UniProt Primary Accession #    P17752 [Other Products]
 UniProt Secondary Accession #    O95188; O95189; Q16736; Q3KPG8; D3DQX6 [Other Products]
 UniProt Related Accession #    P17752 [Other Products]
 Molecular Weight    50,985 Da
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 NCBI Official Full Name    tryptophan 5-hydroxylase 1
 NCBI Official Synonym Full Names    tryptophan hydroxylase 1
 NCBI Official Symbol    TPH1 [Similar Products]
 NCBI Official Synonym Symbols   
TPRH; TRPH
[Similar Products]
 NCBI Protein Information    tryptophan 5-hydroxylase 1; L-tryptophan hydroxylase; tryptophan 5-monooxygenase 1; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)
 UniProt Protein Name    Tryptophan 5-hydroxylase 1
 UniProt Synonym Protein Names   
Tryptophan 5-monooxygenase 1
 Protein Family    Tryptophan 5-hydroxylase
 UniProt Gene Name    TPH1 [Similar Products]
 UniProt Synonym Gene Names    TPH; TPRH; TRPH [Similar Products]
 UniProt Entry Name    TPH1_HUMAN
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 NCBI Summary for TPH1    This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]
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 UniProt Comments for TPH1    TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described.

Protein type: EC 1.14.16.4; Oxidoreductase; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 11p15.3-p14

Cellular Component: neuron projection; cytosol

Molecular Function: amino acid binding; tryptophan 5-monooxygenase activity; iron ion binding

Biological Process: circadian rhythm; aromatic amino acid family metabolic process; indolalkylamine biosynthetic process; negative regulation of ossification; positive regulation of fat cell differentiation; bone remodeling; serotonin biosynthetic process
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 Research Articles on TPH1    1. None of the genetic markers within SLC6A4, MAOA, TPH1 and TPH2 were significantly associated with completed suicide or suicide method in the basic association tests.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TPH1 elisa kitPathways associated with TPH1 elisa kit
 Reference Product  PubMed Publications
 LRP5 elisa kit  >10 publications with TPH1 and LRP5
 DDC elisa kit  >5 publications with TPH1 and DDC
 PRL elisa kit  >2 publications with TPH1 and PRL
 IDO1 elisa kit  >1 publications with TPH1 and IDO1
 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway antibodies  Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway Diagram
 Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway antibodies  Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 Serotonergic Synapse Pathway antibodies  Serotonergic Synapse Pathway Diagram
 Serotonin And Melatonin Biosynthesis Pathway antibodies  Serotonin And Melatonin Biosynthesis Pathway Diagram
Diseases associated with TPH1 elisa kitOrgans/Tissues associated with TPH1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >24 publications with TPH1 and Inflammation
 Schizophrenia Antibodies  >24 publications with TPH1 and Schizophrenia
 Hypertension Antibodies  >17 publications with TPH1 and Hypertension
 Hypertension, Pulmonary Antibodies  >17 publications with TPH1 and Hypertension, Pulmonary
 Nervous System Diseases Antibodies  >16 publications with TPH1 and Nervous System Diseases
 Necrosis Antibodies  >7 publications with TPH1 and Necrosis
 Cardiovascular Diseases Antibodies  >7 publications with TPH1 and Cardiovascular Diseases
 Immune System Diseases Antibodies  >7 publications with TPH1 and Immune System Diseases
 Heart Diseases Antibodies  >5 publications with TPH1 and Heart Diseases
 Carcinoma Antibodies  >4 publications with TPH1 and Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with TPH1 and Bone
 Connective Tissue Antibodies  >4 publications with TPH1 and Connective Tissue
 Heart Antibodies  >3 publications with TPH1 and Heart
 Muscle Antibodies  >3 publications with TPH1 and Muscle
 Brain Antibodies  >3 publications with TPH1 and Brain
 Vascular Antibodies  >2 publications with TPH1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with TPH1 and Embryonic Tissue
 Ear Antibodies  >1 publications with TPH1 and Ear
 Kidney Antibodies  >1 publications with TPH1 and Kidney
 Umbilical Cord Antibodies  >1 publications with TPH1 and Umbilical Cord
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