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TNFSF10 elisa kit :: Human tumor necrosis factor (ligand) superfamily, member 10 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701472
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), ELISA Kit

★Popular Item★
 Also Known As   

Human soluble tumor necrosis factor-related apoptosis inducing ligand, sTRAIL ELISA Kit

 Product Synonym Names    Human soluble tumor necrosis factor-related apoptosis inducing ligand; sTRAIL ELISA KIT; APO2L; Apo-2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family; member 10; tumor necrosis factor apoptosis-inducing ligand splice var; tumor necrosis factor (ligand) superfamily; member 10
 Product Gene Name   

TNFSF10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701472 Testing Data    Testing Data PDF
 OMIM    603598
 3D Structure    ModBase 3D Structure for P50591
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human s TRAIL. No significant cross-reactivity or interference between human s TRAIL and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, urine, cerebrospinal fluid (CSF)
 Assay Type    Quantitative Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    <7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNFSF10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNFSF10 purchase    MBS701472 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing TNFSF10, with an estimated sensitivity of <7.81 pg/ml. The ELISA analytical biochemical technique of the MBS701472 kit is based on TNFSF10 antibody-TNFSF10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFSF10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFSF10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, urine, cerebrospinal fluid (CSF). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNFSF10 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTRAIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTRAIL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTRAIL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTRAIL bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TNFSF10 elisa kit    TNFSF10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701472. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNFSF10. It may not necessarily be applicable to this product.
 NCBI GI #    300193032
 NCBI GeneID    8743
 NCBI Accession #    NP_001177871.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001190942.1 [Other Products]
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 UniProt Primary Accession #    P50591 [Other Products]
 UniProt Secondary Accession #    A1Y9B3 [Other Products]
 UniProt Related Accession #    P50591 [Other Products]
 Molecular Weight    32,509 Da
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 NCBI Official Full Name    tumor necrosis factor ligand superfamily member 10 isoform 2
 NCBI Official Synonym Full Names    tumor necrosis factor (ligand) superfamily, member 10
 NCBI Official Symbol    TNFSF10 [Similar Products]
 NCBI Official Synonym Symbols   
TL2; APO2L; CD253; TRAIL; Apo-2L
[Similar Products]
 NCBI Protein Information    tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta
 UniProt Protein Name    Tumor necrosis factor ligand superfamily member 10
 UniProt Synonym Protein Names   
Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253
 Protein Family    Tumor necrosis factor ligand superfamily
 UniProt Gene Name    TNFSF10 [Similar Products]
 UniProt Synonym Gene Names    APO2L; TRAIL; Apo-2L; Protein TRAIL [Similar Products]
 UniProt Entry Name    TNF10_HUMAN
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 NCBI Summary for TNFSF10    The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
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 UniProt Comments for TNFSF10    Function: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Cofactor: Binds 1 zinc ion per trimer.
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 Research Articles on TNFSF10    1. These results suggest that TRAIL inhibits VEGF-induced angiogenesis by increasing caspase-8 activity and subsequently decreasing non-apoptotic signaling functions of procaspase-8, without inducing caspase-3 activation and endothelial cell cytotoxicity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNFSF10 elisa kitPathways associated with TNFSF10 elisa kit
 Reference Product  PubMed Publications
 TNFRSF10B elisa kit  >2730 publications with TNFSF10 and TNFRSF10B
 CASP8 elisa kit  >1288 publications with TNFSF10 and CASP8
 FADD elisa kit  >624 publications with TNFSF10 and FADD
 TNFRSF10A elisa kit  >556 publications with TNFSF10 and TNFRSF10A
 CFLAR elisa kit  >252 publications with TNFSF10 and CFLAR
 TNFRSF11B elisa kit  >171 publications with TNFSF10 and TNFRSF11B
 TNFRSF10C elisa kit  >117 publications with TNFSF10 and TNFRSF10C
 TRADD elisa kit  >62 publications with TNFSF10 and TRADD
 CASP10 elisa kit  >61 publications with TNFSF10 and CASP10
 TNFRSF10D elisa kit  >52 publications with TNFSF10 and TNFRSF10D
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Caspase-8 Activation By Cleavage Pathway antibodies  Caspase-8 Activation By Cleavage Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Death Receptor Signalling Pathway antibodies  Death Receptor Signalling Pathway Diagram
 Dimerization Of Procaspase-8 Pathway antibodies  Dimerization Of Procaspase-8 Pathway Diagram
Diseases associated with TNFSF10 elisa kitOrgans/Tissues associated with TNFSF10 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >3783 publications with TNFSF10 and Necrosis
 Leukemia Antibodies  >459 publications with TNFSF10 and Leukemia
 Colorectal Neoplasms Antibodies  >339 publications with TNFSF10 and Colorectal Neoplasms
 Liver Diseases Antibodies  >331 publications with TNFSF10 and Liver Diseases
 Breast Neoplasms Antibodies  >305 publications with TNFSF10 and Breast Neoplasms
 Liver Neoplasms Antibodies  >286 publications with TNFSF10 and Liver Neoplasms
 Lung Neoplasms Antibodies  >284 publications with TNFSF10 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >272 publications with TNFSF10 and Neoplasms, Experimental
 Inflammation Antibodies  >264 publications with TNFSF10 and Inflammation
 Glioma Antibodies  >245 publications with TNFSF10 and Glioma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >892 publications with TNFSF10 and Blood
 Liver Antibodies  >358 publications with TNFSF10 and Liver
 Lung Antibodies  >293 publications with TNFSF10 and Lung
 Prostate Antibodies  >273 publications with TNFSF10 and Prostate
 Brain Antibodies  >183 publications with TNFSF10 and Brain
 Vascular Antibodies  >141 publications with TNFSF10 and Vascular
 Kidney Antibodies  >119 publications with TNFSF10 and Kidney
 Skin Antibodies  >94 publications with TNFSF10 and Skin
 Bone Marrow Antibodies  >86 publications with TNFSF10 and Bone Marrow
 Muscle Antibodies  >65 publications with TNFSF10 and Muscle
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