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TNFRSF10A elisa kit :: Human tumor necrosis factor receptor superfamily, member 10a ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704316
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tumor necrosis factor receptor superfamily, member 10a (TNFRSF10A), ELISA Kit

 Also Known As   

Human tumor necrosis factor-related apoptosis-inducing ligand receptor 1, TRAIL-R1 ELISA Kit

 Product Synonym Names    Human tumor necrosis factor-related apoptosis-inducing ligand receptor 1 (TRAIL-R1) ELISA Kit; APO2; CD261; DR4; MGC9365; TRAILR-1; TRAILR1; TNF-related apoptosis inducing ligand receptor 1; cytotoxic TRAIL receptor; death receptor 4; tumor necrosis factor receptor superfamily; member 10a
 Product Gene Name   

TNFRSF10A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704316 Testing Data    Testing Data PDF
 OMIM    603611
 3D Structure    ModBase 3D Structure for O00220
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human TNFRSF10A. No significant cross-reactivity or interference between Human TNFRSF10A and analogues was observed.
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 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Apoptosis/Autophagy
 Protein Biological Process 3    Apoptosis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNFRSF10A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNFRSF10A purchase    MBS704316 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor necrosis factor receptor superfamily, member 10a (TNFRSF10A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNFRSF10A. The ELISA analytical biochemical technique of the MBS704316 kit is based on TNFRSF10A antibody-TNFRSF10A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFRSF10A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFRSF10A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNFRSF10A elisa kit

   Introduction: TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. TRAIL binds at least five different receptors: TRAIL R1 (DR4), R2 (DR5), R3 (DcR1), R4 (DcR2), and osteoprotegerin (OPG). TRAIL receptors all exhibit cysteine-rich repeats characteristic of the TNF receptor superfamily. TRAIL R1 and R2 are type 1 transmembrane proteins with intracellular death domains (DD) and, subsequently, have the ability to mediate TRAIL-induced apoptosis. The remaining receptors are decoys that may regulate TRAIL activity by competing for binding sites with TRAIL R1 and R2. TRAIL R3 is membrane-associated via GPI linkage. TRAIL R4 is a type 1 transmembrane receptor, but exhibits a truncated cytoplasmic domain. Like the active receptors TRAIL R1 and R2, OPG has a DD motif but is a soluble protein.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRAIL-R1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TRAIL-R1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TRAIL-R1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TRAIL-R1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TNFRSF10A elisa kit    TNFRSF10A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704316. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNFRSF10A. It may not necessarily be applicable to this product.
 NCBI GI #    259906438
 NCBI GeneID    8797
 NCBI Accession #    NP_003835.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_003844.3 [Other Products]
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 UniProt Primary Accession #    O00220 [Other Products]
 UniProt Secondary Accession #    Q53Y72; Q96E62; A8K5I4 [Other Products]
 UniProt Related Accession #    O00220 [Other Products]
 Molecular Weight    50,089 Da
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 NCBI Official Full Name    tumor necrosis factor receptor superfamily member 10A
 NCBI Official Synonym Full Names    tumor necrosis factor receptor superfamily, member 10a
 NCBI Official Symbol    TNFRSF10A [Similar Products]
 NCBI Official Synonym Symbols   
DR4; APO2; CD261; TRAILR1; TRAILR-1
[Similar Products]
 NCBI Protein Information    tumor necrosis factor receptor superfamily member 10A; TRAIL-R1; TRAIL receptor 1; death receptor 4; cytotoxic TRAIL receptor; TNF-related apoptosis-inducing ligand receptor 1; tumor necrosis factor receptor superfamily member 10a variant 2
 UniProt Protein Name    Tumor necrosis factor receptor superfamily member 10A
 UniProt Synonym Protein Names   
Death receptor 4; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAIL-R1; CD_antigen: CD261
 Protein Family    Tumor necrosis factor receptor superfamily
 UniProt Gene Name    TNFRSF10A [Similar Products]
 UniProt Synonym Gene Names    APO2; DR4; TRAILR1; TRAIL receptor 1; TRAIL-R1 [Similar Products]
 UniProt Entry Name    TR10A_HUMAN
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 NCBI Summary for TNFRSF10A    The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TNFRSF10A    Function: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.1 PublicationManual assertion based on experiment in:Ref.7
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 Research Articles on TNFRSF10A    1. C626G and A1322G polymorphisms of DR4 are associated with increased cancer risk, but the A683C polymorphism is rarely associated with cancer risk
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNFRSF10A elisa kitPathways associated with TNFRSF10A elisa kit
 Reference Product  PubMed Publications
 TNFSF10 elisa kit  >556 publications with TNFRSF10A and TNFSF10
 CASP8 elisa kit  >189 publications with TNFRSF10A and CASP8
 FADD elisa kit  >105 publications with TNFRSF10A and FADD
 CFLAR elisa kit  >49 publications with TNFRSF10A and CFLAR
 TRADD elisa kit  >20 publications with TNFRSF10A and TRADD
 CASP10 elisa kit  >12 publications with TNFRSF10A and CASP10
 RIPK1 elisa kit  >7 publications with TNFRSF10A and RIPK1
 TP53 elisa kit  >5 publications with TNFRSF10A and TP53
 IKBKB elisa kit  >3 publications with TNFRSF10A and IKBKB
 ARAP1 elisa kit  >1 publications with TNFRSF10A and ARAP1
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
Diseases associated with TNFRSF10A elisa kitOrgans/Tissues associated with TNFRSF10A elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >513 publications with TNFRSF10A and Necrosis
 Adenocarcinoma Antibodies  >66 publications with TNFRSF10A and Adenocarcinoma
 Leukemia Antibodies  >56 publications with TNFRSF10A and Leukemia
 Lung Neoplasms Antibodies  >33 publications with TNFRSF10A and Lung Neoplasms
 Breast Neoplasms Antibodies  >32 publications with TNFRSF10A and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >32 publications with TNFRSF10A and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >29 publications with TNFRSF10A and Neoplasms, Experimental
 Liver Diseases Antibodies  >28 publications with TNFRSF10A and Liver Diseases
 Colonic Neoplasms Antibodies  >25 publications with TNFRSF10A and Colonic Neoplasms
 Head and Neck Neoplasms Antibodies  >22 publications with TNFRSF10A and Head and Neck Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >71 publications with TNFRSF10A and Blood
 Liver Antibodies  >34 publications with TNFRSF10A and Liver
 Lung Antibodies  >33 publications with TNFRSF10A and Lung
 Prostate Antibodies  >27 publications with TNFRSF10A and Prostate
 Brain Antibodies  >17 publications with TNFRSF10A and Brain
 Kidney Antibodies  >13 publications with TNFRSF10A and Kidney
 Vascular Antibodies  >13 publications with TNFRSF10A and Vascular
 Skin Antibodies  >9 publications with TNFRSF10A and Skin
 Pancreas Antibodies  >7 publications with TNFRSF10A and Pancreas
 Intestine Antibodies  >7 publications with TNFRSF10A and Intestine
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