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UBA1 elisa kit :: Human ubiquitin-like modifier activating enzyme 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702666
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ubiquitin-like modifier activating enzyme 1 (UBA1), ELISA Kit

 Also Known As   

Human E1/ubiquitin-activating enzyme, E1/UBAE ELISA Kit

 Product Synonym Names    Human E1/ubiquitin-activating enzyme; E1/UBAE ELISA Kit; CTD-2522E6.1; A1S9; A1S9T; A1ST; AMCX1; GXP1; MGC4781; SMAX2; UBA1A; UBE1; UBE1X; A1S9T and BN75 temperature sensitivity complementing; UBA1; ubiquitin-activating enzyme E1 homolog A; ubiquitin-activa; ubiquitin-like modifier activating enzyme 1
 Product Gene Name   

UBA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702666 Testing Data    Testing Data PDF
 OMIM    314370
 3D Structure    ModBase 3D Structure for P22314
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human UBA1. No significant cross-reactivity or interference between Human UBA1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Detection Range    47 pg/ml-3000 pg/ml
 Sensitivity    11.75 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Ubiquitin
 Protein Biological Process 3    Ubl conjugation pathway
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of UBA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for UBA1 purchase    MBS702666 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ubiquitin-like modifier activating enzyme 1 (UBA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 47 pg/ml-3000 pg/ml in biological research samples containing UBA1, with an estimated sensitivity of 11.75 pg/ml. The ELISA analytical biochemical technique of the MBS702666 kit is based on UBA1 antibody-UBA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UBA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UBA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for UBA1 elisa kit

   Introduction: Ubiquitin-activating enzymes, also known as E1 enzymes, catalyze the first step in the ubiquitination reaction that targets a protein for degradation via the proteasome. The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell. The E1 reaction is itself ATP-dependent and begins with ATP hydrolysis coupled to adenylation of a molecule of free ubiquitin. The adenylated ubiquitin is transferred to the E1's active site cysteine residue in concert with the adenylation of a second molecule of ubiquitin, which is then transferred to the active-site cysteine residue of the next enzyme in the ubiquitination catalytic chain, the ubiquitin-conjugating enzyme or E2. The reaction in which the ubiquitin is attached to the target protein is performed by the third class of molecules in the chain, the ubiquitin ligases or E3s. In most cells there are only a few different types of E1 and E2 enzymes, but many different types of E3s. It is the E3 that binds the substrate and therefore confers substrate specificity.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of UBA1 elisa kit    UBA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702666. Click to request current manual
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NCBI/Uniprot data below describe general gene information for UBA1. It may not necessarily be applicable to this product.
 NCBI GI #    23510338
 NCBI GeneID    7317
 NCBI Accession #    NP_003325.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003334.3 [Other Products]
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 UniProt Primary Accession #    P22314 [Other Products]
 UniProt Secondary Accession #    Q5JRR8; Q96E13 [Other Products]
 UniProt Related Accession #    P22314 [Other Products]
 Molecular Weight    117,849 Da
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 NCBI Official Full Name    ubiquitin-like modifier-activating enzyme 1
 NCBI Official Synonym Full Names    ubiquitin-like modifier activating enzyme 1
 NCBI Official Symbol    UBA1 [Similar Products]
 NCBI Official Synonym Symbols   
A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
[Similar Products]
 NCBI Protein Information    ubiquitin-like modifier-activating enzyme 1; POC20 centriolar protein homolog; UBA1, ubiquitin-activating enzyme E1 homolog A; A1S9T and BN75 temperature sensitivity complementing
 UniProt Protein Name    Ubiquitin-like modifier-activating enzyme 1
 UniProt Synonym Protein Names   
Protein A1S9; Ubiquitin-activating enzyme E1
 Protein Family    Ubiquitin-activating enzyme
 UniProt Gene Name    UBA1 [Similar Products]
 UniProt Synonym Gene Names    A1S9T; UBE1 [Similar Products]
 UniProt Entry Name    UBA1_HUMAN
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 NCBI Summary for UBA1    The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for UBA1    Function: Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
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 Research Articles on UBA1    1. Diminished ubiquitylation phenotype observed in enteropathogenic Escherichia coli infected cells corresponds to a strong reduction in the abundance of both Ube1 and Uba6.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with UBA1 elisa kitPathways associated with UBA1 elisa kit
 Reference Product  PubMed Publications
 UBA2 elisa kit  >15 publications with UBA1 and UBA2
 CDC34 elisa kit  >1 publications with UBA1 and CDC34
 SMO elisa kit  >1 publications with UBA1 and SMO
 UBC elisa kit  >1 publications with UBA1 and UBC
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Parkin-Ubiquitin Proteasomal System Pathway antibodies  Parkin-Ubiquitin Proteasomal System Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
Diseases associated with UBA1 elisa kitOrgans/Tissues associated with UBA1 elisa kit
 Disease Name  Pubmed Publications
 Atrophy Antibodies  >7 publications with UBA1 and Atrophy
 Nervous System Diseases Antibodies  >5 publications with UBA1 and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with UBA1 and Brain Diseases
 Weight Loss Antibodies  >3 publications with UBA1 and Weight Loss
 Necrosis Antibodies  >3 publications with UBA1 and Necrosis
 Adenocarcinoma Antibodies  >2 publications with UBA1 and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >2 publications with UBA1 and Cardiovascular Diseases
 Skin Diseases Antibodies  >1 publications with UBA1 and Skin Diseases
 Nerve Degeneration Antibodies  >1 publications with UBA1 and Nerve Degeneration
 Inflammation Antibodies  >1 publications with UBA1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with UBA1 and Bone
 Connective Tissue Antibodies  >4 publications with UBA1 and Connective Tissue
 Heart Antibodies  >3 publications with UBA1 and Heart
 Muscle Antibodies  >3 publications with UBA1 and Muscle
 Brain Antibodies  >3 publications with UBA1 and Brain
 Vascular Antibodies  >2 publications with UBA1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with UBA1 and Embryonic Tissue
 Ear Antibodies  >1 publications with UBA1 and Ear
 Kidney Antibodies  >1 publications with UBA1 and Kidney
 Umbilical Cord Antibodies  >1 publications with UBA1 and Umbilical Cord
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